FORMULATION, ANTIOXIDANT, AND ANTI-AGING ACTIVITY OF RUBUS FRAXINIFOLIUS FRACTION

Authors

  • SULISTYOWATI Laboratory of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok-16424, West Java, Indonesia https://orcid.org/0009-0007-2717-1036
  • BERNA ELYA Laboratory of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok-16424, West Java, Indonesia https://orcid.org/0000-0003-2904-6515
  • SYAMSU NUR Laboratory of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok-16424, West Java, Indonesia. Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Health, Almarisah Madani University, Makassar, South Sulawesi, Indonesia https://orcid.org/0000-0001-7730-4414
  • RADITYA ISWANDANA Laboratory of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Depok-16424, West Java, Indonesia https://orcid.org/0000-0003-2637-3844

DOI:

https://doi.org/10.22159/ijap.2024v16i4.51013

Keywords:

Antioxidant, Gel, Rubus fraxinifolius leaves, Elastase inhibition

Abstract

Objective: The aim of this research was to evaluate the antioxidant activity and elastase inhibition of the Rubus gel fraction as well as its physical stability for 12 w. Rubus fraxinifolius leaves were reported to have strong antioxidant and elastase enzyme inhibitory activity.

Methods: Water fraction from ultrasonic-assisted extraction (UAE) of old Rubus leaves was used and formulated into face gel preparations. Antioxidant activity 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and 2,2'-azino-bisphenol S (2,2'-ABPS) (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), elastase inhibition, and stability test during 12 w of storage, both at room temperature (30±2 °C) or accelerated temperature (40±2 °C) were performed.

Results: The anti-aging gel showed better antioxidant activity and elastase inhibition on F1 (3%) at both conditions compared to F2 (4%) and F3 (5%). The physical stability test met the requirements. However, there was a slight decrease in antioxidant activity and elastase inhibition after 12 w of storage at 30±2 °C and 40±2 °C (F1, F2, and F3).

Conclusion: The F1 met the standards and was relatively stable at 30±2 °C during 12 w of storage.

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References

Amelia R, Guskarnali MRG, Niani CR, Lewaherilla N. Predicting particulate matter PM2.5 using the exponential smoothing and Seasonal Arima with R studio. IOP Conf Ser Earth Environ Sci. 2022;1108(1).

Getoff N. Anti-aging and aging factors in life. The role of free radicals. Radiat Phys Chem. 2007;76(10):1577-86. doi: 10.1016/j.radphyschem.2007.01.002.

Draelos ZD. The latest cosmeceutical approaches for anti-aging. J Cosmet Dermatol. 2007;6Suppl 1:2-6. doi: 10.1111/j.1473-2165.2007.00313.x.

Ribeiro AS, Estanqueiro M, Oliveira MB, Sousa Lobo J. Main benefits and applicability of plant extracts in skin care products. Cosmetics. 2015;2(2):48-65. doi: 10.3390/cosmetics2020048.

Anggraini NB, Elya B, Iskandarsyah I. Antielastase activity of macassar kernels (Rhus javanica) stem extract and skin elasticity evaluation of its topical gel formulation. Adv Pharmacol Pharm Sci. 2021;2021:6690029. doi: 10.1155/2021/6690029, PMID 33977273.

Hughes K, Ho R, Butaud JF, Filaire E, Ranouille E, Berthon JY. A selection of eleven plants used as traditional polynesian cosmetics and their development potential as anti-aging ingredients, hair growth promoters and whitening products. J Ethnopharmacol. 2019;245:112159. doi: 10.1016/j.jep.2019.112159, PMID 31419502.

Desmiaty Y, Hanafi M, Saputri FC, Elya B, Rifai EA, Syahdi RR. Two triterpenoids from rubus fraxinifolius leaves and their tyrosinase and elastase inhibitory activities. Sci Rep. 2021;11(1):20452. doi: 10.1038/s41598-021-99970-x, PMID 34650166.

Sulistyowati EB, Iswandana R, Nur S. Phytocompounds and in vitro antiaging activity of ethanolic extract and fractions of Rubus fraxinifolius poir. leaves. Journal of Pharmacy & Pharmacognosy Research. 2023;11(4):595-610.

Rathod HJ, Mehta DP. A review on pharmaceutical gel. Int J Pharm Sci. 2015;1(1):33-47.

Ciurba A. Development and evaluation of Bigels containing naproxen sodium for topical administration. Farmacia. 2023;71(4):771-80. doi: 10.31925/farmacia.2023.4.13.

Desmiaty Y, Faizatun F, Noviani Y, Ratih H, Ambarwati NS. Review article: potential of natural products in inhibiting premature skin aging. Int J Appl Pharm. 2022;14;Special Issue 3:1-5.

Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot D. Phytochemicals: extraction, isolation, and identification of bioactive compounds from plant extracts. Plants. 2017;6(4). doi: 10.3390/plants6040042.

Al-Haj Ibrahim H. Introductory chapter: fractionation. Fractionation. 2019.

Ismed F, Desti WN, Arifa N, Rustini R, Putra DP. TLC-bioautographic and lC-MS/MS detection of antimicrobial compounds from four semipolar extracts of Cladonia species. Proceedings of the 2nd int conf Contemp sci clin pharm. 2021;2022:40-9. doi: 10.2991/ahsr.k.211105.008.

Husain F, Ysrafil Y, Daud RP, Aisyah AN, Fadri A, Nur S. Authentication of medicinal chemicals in traditional herbal products (Jamu) by UV-vis spectrophotometry. HUJPHARM. 2023;43(2):100-10. doi: 10.52794/hujpharm.1090673.

Gunarti N, Aisyah I, lia F. Physical stability test sunscreen gel extracts black berry fruit (Rubus fruticosus l.). IOP Conf Ser: Mater Sci Eng. 2021;1071(1):012011. doi: 10.1088/1757-899X/1071/1/012011.

Kausar R, Akhtar N. Formulation of an emulgel containing strawberry fruit extract and in vivo evaluation for different skin parameter. Indian J Pharm Educ Res. 2017;51(4):679-83. doi: 10.5530/ijper.51.4.100.

Setiawan F, Nurdianti L, Sri N. Formulation and effectivity of the antioxidant gel preparation containing zeaxanthin as anti-aging. Int J App Pharm. 2021;13Special Issue 4:152-6. doi: 10.22159/ijap.2021.v13s4.43846.

Parmer C. What is viscosity; 2022. Available from: https://www.coleparmer.com/tech-article/what-is-viscosity.

Nur S, Setiawan H, Hanafi M, Elya B. Phytochemical composition, antioxidant, in vitro and in silico studies of active compounds of curculigo latifolia extracts as promising elastase inhibitor. Saudi J Biol Sci. 2023;30(8):103716. doi: 10.1016/j.sjbs.2023.103716, PMID 37457237.

ICH. Q1A(R2). Ich Harmon Tripart Guidel. International Conference on Harmonization (ICH). Guidance for industry: Q1A(R2) Stability Testing of New Drug Substances and Products; 2003;4:24.

Nur S, Aswad M, Yulianty R, Burhan A, Khairi N, Sami F. The antioxidant and anti-ageing activity of lyophilisate kersen. Food Res. 2023;7(Apr):23-30.

Nechita MA. Polyphenolic compounds analysis and antioxidant activity of two Romanian cultivated agastache species. Farmacia. 2023;71(4):704-9. doi: 10.31925/farmacia.2023.4.6.

Syarifah A, Budiman A, Nazilah SA. Formulation and antioxidant activity of serum gel of ethyl acetate fraction from Musa x paradisiaca l. Proceedings of the 4th int conf sustain innov. 2020-Health Sci Nurs (ICoSIHSN 2020). 2021;33:310-5.

Aryantini D, Kristianingsih I, Kurniawati E, Dewanti T. Indian Journal of Pharmaceutical and Biological Research (IJPBR) formulation. Physical Characteristics and Irritation Test of Soothing Gel Combination from Aloe vera gel and Dragon Fruit Juice. Indian J Pharm Biol Res. 2020;8(3):19-25. doi: 10.30750/ijpbr.8.3.3.

Minatel IO, Borges CV, Ferreira MI, Gomez HA, Chen CY, lima GP. Phenolic compounds: functional properties, impact of processing and bioavailability. Phenolic Compd-Biol Act; 2017.

Xiao F, Xu T, Lu B, Liu R. Guidelines for antioxidant assays for food components. Food Front. 2020;1(1):60-9.

Shahidi F, Zhong Y. Measurement of antioxidant activity. J Funct Foods. 2015;18:757-81. doi: 10.1016/j.jff.2015.01.047.

Harahap A, Triamarta S, Kharisma D, Hanifah W, Iqbal M, Arifa N. Evaluation of the anti-tyrosinase-anti-aging potential and metabolite profiling from the bioactive fraction of corn cob (Zea mays l.). Int J App Pharm. 2024;16;Special Issue 1:71-6. doi: 10.22159/ijap.2024.v16s1.18.

Campbell KL, Lichtensteiger CA. Structure and function of the skin. Small Anim Dermatol Secrets. 2003:1-9.

Bisswanger H. Enzyme assays. Perspect Sci. 2014;1(1-6):41-55. doi: 10.1016/j.pisc.2014.02.005.

Salehiyan R, Bandyopadhyay J, Ray SS. Mechanism of thermal degradation-induced gel formation in polyamide 6/ethylene vinyl alcohol blend nanocomposites studied by time-resolved rheology and hyphenated thermogravimetric analyzer fourier transform infrared spectroscopy mass spectroscopy: synergistic role of nanoparticles and maleic-anhydride-grafted polypropylene. ACS Omega. 2019;4(5):9569-82. doi: 10.1021/acsomega.9b00940, PMID 31460048.

Published

07-07-2024

How to Cite

SULISTYOWATI, ELYA, B., NUR, S., & ISWANDANA, R. (2024). FORMULATION, ANTIOXIDANT, AND ANTI-AGING ACTIVITY OF RUBUS FRAXINIFOLIUS FRACTION. International Journal of Applied Pharmaceutics, 16(4), 121–128. https://doi.org/10.22159/ijap.2024v16i4.51013

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