CHEMOMETRIC ASSISTED QUANTITATIVE DETERMINATION OF VITEXIN IN ETHANOLIC EXTRACT OF BINAHONG (ANREDERA CORDIFOLIA (TEN.) STEENIS) LEAVES

OCTAVIANUS BUDI SANTOSA; MICHAEL RAHARJA GANI; SRI HARTATI YULIANI

doi:10.22159/ijap.2020v12i6.39248

Authors

OCTAVIANUS BUDI SANTOSA Faculty of Pharmacy, Sanata Dharma University, Paingan, Maguwoharjo, Depok, Sleman, Yogyakarta 55284, Indonesia
MICHAEL RAHARJA GANI Faculty of Pharmacy, Sanata Dharma University, Paingan, Maguwoharjo, Depok, Sleman, Yogyakarta 55284, Indonesia
SRI HARTATI YULIANI Faculty of Pharmacy, Sanata Dharma University, Paingan, Maguwoharjo, Depok, Sleman, Yogyakarta 55284, Indonesia

DOI:

https://doi.org/10.22159/ijap.2020v12i6.39248

Keywords:

Binahong, HPLC, UV Spectroscopy, Vitexin

Abstract

Objective: The objective of this study was to develop a UV spectroscopy method in combination with multivariate analysis for determining vitexin in binahong (Anredera cordifolia (Ten.) Steenis) leaves extract.

Methods: The partial least square (PLS) regression and the principal component regression (PCR) was performed in this study to evaluate several statistical performances such as coefficient of determination (R²), root mean square error of calibration (RMSEC), root mean square error of cross-validation (RMSECV), root mean square error of prediction (RMSEP) and relative error of prediction (REP). Cross-validation in this study was performed using leave one out technique.

Results: The R² values of calibration data sets resulted from PLS and PCR method were 0.9675 and 0.9648, respectively. The low values of RMSEC and RMSECV both for PLS and PCR method indicated the minimum error of the calibration models. The R² values of validation data sets resulted from PLS and PCR method were 0.9778 and 0.9820, respectively. The low values of RMSEP both for PLS and PCR method indicated the minimum error of prediction generated from the calibration data sets. Multivariate calibration techniques were applied to determine the content of vitexin in binahong leaves extract. Predicted values from the multivariate calibration models were compared to the actual values determined from a validated HPLC method. It was found that PLS models resulted in the lowest REP values compared to the PCR models.

Conclusion: The chemometrics technique can be applied as an alternative method for determining vitexin levels in the ethanol solution of binahong leaves extract.

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