PHYSICOCHEMICAL PROPERTIES AND NUTRITION OF MORINGA OLEIFERA LAM. LEAF EXTRACT: A PRELIMINARY STUDY ON PREPARATION PHYTOSOMES AS HERBAL SUPPLEMENT FOR CHILDREN

MUTIA SARI WARDANA; MAHDI JUFRI; ABDUL MUN’IM

doi:10.22159/ijap.2022v14i1.43477

Authors

MUTIA SARI WARDANA Graduate of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Indonesia https://orcid.org/0000-0002-4132-8729
MAHDI JUFRI Department of Pharmaceutical Technology and Drug Development, Faculty of Pharmacy, Universitas Indonesia
ABDUL MUN’IM Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia

DOI:

https://doi.org/10.22159/ijap.2022v14i1.43477

Keywords:

Moringa oleifera, Stunting, Supplement, Phytosome, Amino acid

Abstract

Objective: The study aimed to evaluate the physicochemical properties and nutrition of Moringa leaf extract. In addition, the preliminary study for the preparation of Moringa leaf extract-loaded phytosomes for a supplement.

Methods: Extraction of Moringa leaf made using microwave-assisted extraction, followed by evaluation of proximate analysis (water, total ash, acid-insoluble of ash contents, and residual n-hexane), phytochemical screening, and nutrition such as crude protein, amino acids, and minerals (iron, zinc, and calcium). The phytosomes were prepared by the anti-solvent precipitation method and assessed for the morphology, particle size, zeta potential, polydispersity index (PDI), entrapment efficiency (EE), and Fourier-transform infrared spectra.

Results: The nutrition contents of crude protein, iron, zinc, and calcium were 19.61±0.07%, 3.47±0.00 mg/100g, 5.46±0.05 mg/100g, and 747.40±4.89 mg/100g, respectively. The amino acids with the highest concentrations were glutamic acid, phenylalanine, aspartic acid, alanine, and arginine in the extract. The best preparation using sonication 10 min by morphology was a spherical included particle size, PDI, zeta potential, and EE of arginine was 87.16±1.73 nm, 0.22±0.04, −23.07±0.76 mV, and 108.94±0.52%, respectively.

Conclusion: These preliminary results provide evidence of the nutritional benefit of Moringa leaf extract-loaded phytosomes as a promising supplement to prevent stunting in children.

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