VALIDATION OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY FOR DETERMINING THE LEVELS OF CURCUMIN IN TABLET PREPARATIONS

NOVI YANTIH; RIFANI FAUZIA WILTON; DIAH KARTIKA PRATAMI; DESI NADYA AULENA; SHIRLY KUMALA

doi:10.22159/ijap.2022.v14s1.12

Authors

NOVI YANTIH Faculty of Pharmacy, Pancasila University, South Jakarta, 12640, Indonesia
RIFANI FAUZIA WILTON Faculty of Pharmacy, Pancasila University, South Jakarta, 12640, Indonesia
DIAH KARTIKA PRATAMI Faculty of Pharmacy, Pancasila University, South Jakarta, 12640, Indonesia
DESI NADYA AULENA Faculty of Pharmacy, Pancasila University, South Jakarta, 12640, Indonesia
SHIRLY KUMALA Faculty of Pharmacy, Pancasila University, South Jakarta, 12640, Indonesia

DOI:

https://doi.org/10.22159/ijap.2022.v14s1.12

Keywords:

Validation, Curcumin, Tablets, HPLC

Abstract

Objective: The purpose of this research was to optimize and validate HPLC method for determining the curcumin content of tablet preparations in less than ten minutes.

Methods: Curcumin can be analyzed qualitatively or quantitatively using reverse phase HPLC equipped with an isocratic elution system. Furthermore, this research used a visible detector, which contained octadecyl (C18) in its stationary phase at a wavelength of 428 nm and a mobile phase of acetonitrile-acetic acid-aquabides (60:1:39) mixture with a flow rate of 1.0 ml/minute. The chromatographic setup can separate curcuminoids, such as bisdemethoxycurcumin, demethoxycurcumin, and curcumin in less than 10 min. The method is valid on the basis of its selectivity, linearity, accuracy, and precision.

Results: The results showed that there was a high degree of selectivity with a resolution value (Rs) of 1.670 and a high degree of linearity with a correlation coefficient (r) of 0.9915 over a range of 1.38-2.04 µg/ml. Curcumin had a recovery test value of 100.73±1.04%. The curcumin content of the tablet preparations by producing A and B were 3.99±0.02% and 3.97±0.01%, respectively.

Conclusion: According to the results, HPLC is a valid method for determining curcumin levels in tablet preparations in accordance with ICH specifications (International Conference on Harmonization).

Downloads

Download data is not yet available.

References

Lusida MI, Juniastuti YY, Yano Y. Current hepatitis B virus infection situation in Indonesia and its genetic diversity. World J Gastroenterol. 2016;22(32):7264-74. doi: 10.3748/wjg.v22.i32.7264, PMID 27621573.

Marinda FD. Hepatoprotective effect of curcumin in chronic hepatitis. J Majority. 2014;3:52-6.

Jager R, Lowery RP, Calvanese AV, Joy JM, Purpura M, Wilson JM. Comparative absorption of curcumin formulations. Nutr J. 2014;13:11. doi: 10.1186/1475-2891-13-11, PMID 24461029.

Holkar VV, Deepika RN, Sonali KM. Development and validation of UV spectrophotometric method for the estimation of curcumin in bulk and pharmaceutical formulation. World J Pharm Pharm Sci. 2015;4:1456-63.

Faridah WS. Penetapan kadar kurkumin dalam tablet temulawak (Curcuma xanthorriza Roxb.) secara kromatografi lapis tipis densitometri. J Tumbuhan Obat Indones. 2012;5:37-44.

Widjaja M. Validasi metode penetapan kadar kurkumin dalam sediaan cair obat herbal terstanda merk kiranti® secara kromatografi cair kinerja tinggi fase terbalik (skripsi). Yogyakarta: Fakultas Farmasi Universitas Sanata Dharma; 2011.

Cahyono B, Huda MDK, Limantara L. Pengaruh proses pengeringan rimpang temulawak (Curcuma xanthorriza ROXB) terhadap kandungan dan komposisi kurkuminoid. Reaktor. 2003;13(3):157-71 doi: 10.14710/reaktor.13.3.165-171.

Adawiyah L, Kurkumin VMA. Dalam plasma in vitro secara kromatografi cair kinerja tinggi (skripsi). Depok: Fakultas Matematika dan Ilmu Pengetahuan Alam program studi Sarjana Farmasi Universitas Indonesia; 2011.

Kumudhavalli MV, Saravanan C, Thamizh M, Jayakar B. Analytical method development and validation of curcumin in tablet dosage form by RP-HPLC method. Int Res J Pharm. 2011;2:233-6.

Departemen Kesehatan Republik Indonesia. Farmakope Indonesia Edisi, Jakarta V: Direktorat Jendral Pengawasan Obat dan Makanan. 2014. p. 1539.

Yuwono M, Indrayanto G. Validation of chromatographic methods of analysis. Profiles Drug Subst Excip Relat Methodol. 2005;32:243-59. doi: 10.1016/S0099-5428(05)32009-0, PMID 22469089.

Korany MA, Haggag RS, Ragab MAA, Elmallah OA. A validated stability-indicating HPLC method for simultaneous determination of silymarin and curcumin in various dosage forms. Arab J Chem. 2017;10:S1711-25. doi: 10.1016/j.arabjc.2013.06.021.

Reolon JB, Maicon B, Sandra EH, Eduardo AB, Marcelo DM, Leticia MC. Development an validation of high-performance liquid chromatography method for simultaneous determination of acyclovir and curcumin in polymeric microparticles. J Appl Pharm Sci. 2018;10:136-41.

DE AK, Bera T. Analytical method development, validation and stability studies by RP-HPLC method for simultaneous estimation of andrographolide and curcumin in co-encapsulated nanostructured lipid carrier drug delivery system. Int J Appl Pharm. 2021;13:73-86.

Kadam PV, Yadav KN, Bhingare CL, Patil MJ. Standardization and quantification of curcumin from Curcuma longa extract using UV visible spectroscopy and HPLC. J Pharmacogn Phytochem. 2018;7:1913-8.