MICROENCAPSULATION OF INDONESIAN POLYMER BIODIVERSITY IN WARTHON'S JELLY MESENCHYMAL STEM CELL (WJMSC)

DENI RAHMAT; WAHYU WIDOWATI; AHMAD FARIED; ITA MARGARETHA NAINGGOLAN; DIDIK PRIYANDOKO; ANARISA BUDIATI; TEDDY MARCUS ZAKARIA; ERVI AFIFAH

doi:10.22159/ijap.2022.v14s3.19

Authors

DENI RAHMAT Faculty of Pharmacy, Pancasila University, Jl Srengseng Sawah, Jagakarsa, South Jakarta 12640, Indonesia
WAHYU WIDOWATI Faculty of Medicine, Maranatha Christian University, Jl Surya Sumantri No 65, Bandung 40164, West Java, Indonesia
AHMAD FARIED Department of Neurosurgery and, Oncology and Stem Cell Working Group, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin Hospital, Bandung 40161, West Java, Indonesia
ITA MARGARETHA NAINGGOLAN Faculty of Medicine and Health Sciences, Atma Jaya Catholic University, Jl. Jenderal Sudirman No.51, South Jakarta 12930, Indonesia
DIDIK PRIYANDOKO Faculty of Mathematic and Natural Science Education, Indonesia University of Education, Jl. Dr. Setiabudi No. 229, Bandung 40154, West Java, Indonesia
ANARISA BUDIATI Faculty of Pharmacy, Pancasila University, Jl Srengseng Sawah, Jagakarsa, South Jakarta 12640, Indonesia
TEDDY MARCUS ZAKARIA Faculty of Information Technology, Maranatha Christian University, Jl. Surya Sumantri No 65, Bandung 40164, West Java, Indonesia
ERVI AFIFAH PT Aretha Medika Utama, Jl. Babakan Jeruk II No. 9, Bandung 40163, West Java, Indonesia

DOI:

https://doi.org/10.22159/ijap.2022.v14s3.19

Keywords:

WJMSC, Microencapsulation, Polymers, Stem cells, Drug delivery

Abstract

Objective: WJMSC is a stem cell-derived from Wharton's Jelly the umbilical cord of pregnant women which has the ability to differentiate into other cells that are osetogenic, myogenic, neurogenic, and hematopoietic. Stem cell microencapsulation is a cell coating technique that is expected to act as a delivery vehicle. This study aims to make stem cell microencapsulation using various types of natural polymers.

Methods: WJMSC (Wharton's Jelly Mesenchymal Stem Cell) was cultured with supplemented Modified Eagle Medium (MEM) Alpha in incubator with 37 °C and 5% of CO₂. In this study, we investigated various of natural polymers (chitosan, glucomannan, inulin, fucoidan and amylopectin) in WJMSC microencapsulation. Viability cell of WJMSC microencapsulate was measured using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay, and the condition medium (CM) was determined the measure EGF, IL-6, VEGF with ELISA.

Results: WJMSC microencapsulation using chitosan, oxidized glucomannan, inulin, fucoidan and oxidized amylopectin showed viability cell up to 100%. The EGF, IL-6, VEGF levels were increased in all tested polymers compared to negative control.

Conclusion: Tested polymers (chitosan, oxidized glucomannan, inulin, fucoidan, oxidized amylopectin) were not toxic to WJMSC and cell microencapsulation was successfully carried out.

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