PREPARATION AND JUSTIFICATION OF NANOFIBRES-LOADED MAFENIDE USING ELECTROSPINNING TECHNIQUE TO CONTROL RELEASE

SADEQ R. ATIYAH; SARMAD AL-EDRESI

doi:10.22159/ijap.2024v16i2.49691

Authors

SADEQ R. ATIYAH Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, University of Kufa, Najaf-52001, Iraq. Najaf Health Directorate, Al-Amal Hospital, Najaf, Iraq https://orcid.org/0009-0002-0189-3739
SARMAD AL-EDRESI Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, University of Kufa, Najaf-52001, Iraq https://orcid.org/0000-0002-9235-2948

DOI:

https://doi.org/10.22159/ijap.2024v16i2.49691

Keywords:

Nanofibres, Mafenide, Electrospinning, PVA, HPMC, Chitosan

Abstract

Objective: The primary objective was to fabricate a novel drug delivery system capable of providing a controlled and prolonged release of antibiotics.

Methods: The experimental design was formulated using Design-Expert® software (version 13), enabling systematic and efficient fabrication process optimization. The study involved the preparation of various nanofiber formulations with different ratios of the three polymers to assess their impact on drug release behavior. Mafenide, a widely used antibiotic, was chosen as the model drug for this investigation. The electrospinning process allowed for producing uniform and fine nanofibers with a high surface area, ensuring a large drug-loading capacity. The synthesized nanofibers were characterized using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) to evaluate their morphology, chemical interactions, and thermal properties. The drug release kinetics of the antibiotic-loaded nanofibers were studied under different physiological conditions to assess their sustained release behavior.

Results: The final nanofiber formula was successfully prepared using the electrospinning technique. The Fourier Transform Infrared Spectroscopy (FTIR) analysis was achieved to confirm the possibility of chemical interaction and bond formation between mafenide and the polymers. Present. The SEM picture of the optimized nanofiber formula showed the homogeneity and excellent entanglement of the electrospun nanofibers at a resolution of 5 µm. PVA/chitosan/HPMC and mafenide pure drug have been successfully fabricated with sufficient strength to resist swelling after absorbing wound exudate. The polymer network becomes more compact when chitosan and Hydroxypropyl Methyl Cellulose (HPMC) are combined with polyvinyl alcohol (PVA), enabling regulated swelling during solvent ingress. The polymer composite's three-dimensional network influenced how quickly the medication was released from the matrix. Sample 2's polymer network traps the medication, gradually releasing after controlled swelling, resulting in a sustained release profile compared to blank sample according to the cumulative release (%) study of mafenide loaded nanofiber and mafenide drug blank sample.

Conclusion: This research successfully demonstrated the fabrication of sustained-release antibiotic nanofibers using electrospinning and three biocompatible polymers. The systematic optimization approach using Design-Expert® software proved effective in tailoring the drug release behavior of nanofibers. The developed drug delivery system holds great promise for pharmaceutical applications, particularly in improving antibiotic therapies and patient care.

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References

Kanthakumar A, Jayavarthinni M. Antibiotic resistance pattern of Pseudomonas aeruginosa isolated from various clinical samples in a tertiary Care Hospital, Puducherry. 2022;15(2):438-9. doi: 10.22159/ajpcr.2022v15i2.43839.

Mirzaeei S, Faryadras FB, Mehrandish S, Rezaei L, Daneshgar F, Karami A. Development and evaluation of polycaprolactone-based electrospun nanofibers containing timolol maleate as a sustained-release device for treatment of glaucoma: in vivo evaluation in equine eye. Res Pharm Sci. 2022;17(5):468-81. doi: 10.4103/1735-5362.355196, PMID 36386485.

Sarmad S, Aledresi IFA. Razzaq, Abulfadhel J Alshaibani. Enhancing the solubility of nimesulide by loading to a nanoemulsion. Lat Am J Pharm. 2020;39(11):2299-308.

Serrano Garcia W, Bonadies I, Thomas SW, Guarino V. New insights to design electrospun fibers with tunable electrical conductive–semiconductive properties. Sensors (Basel). 2023;23(3). doi: 10.3390/s23031606, PMID 36772646.

Manasa MT, Ramanamurthy KV, Bhupathi PA. Electrospun nanofibrous wound dressings: a review on chitosan composite nanofibers as potential wound dressings. Int J App Pharm. 2023Jul;15(4):1-11. doi: 10.22159/ijap.2023v15i4.47912.

Luraghi A, Peri F, Moroni L. Electrospinning for drug delivery applications: a review. J Control Release. 2021 Jun 10;334:463-84. doi: 10.1016/j.jconrel.2021.03.033, PMID 33781809.

Al-Hamadani MH, Al-Edresi S. Formulation and characterization of hydrogel of proniosomes loaded diclofenac sodium. Int J Drug Deliv Technol. 2022 Jan;12(1):132-6. doi: 10.25258/ijddt.12.1.24.

KN, Rout CS. Conducting polymers: a comprehensive review on recent advances in synthesis, properties and applications. RSC Adv. 2021 Feb;11(10):5659-97. doi: 10.1039/d0ra07800j, PMID 35686160.

Yu H, Zhou Q, He D, Yang J, Wu K, Chai X. Enhanced mechanical and functional properties of chitosan/polyvinyl alcohol/hydroxypropyl methylcellulose/alizarin composite film by incorporating cinnamon essential oil and tea polyphenols. Int J Biol Macromol. 2023 Dec;253(4):126859. doi: 10.1016/j.ijbiomac.2023.126859, PMID 37714243.

Najafi M, Rahimi R. Synthesis of novel Zr-MOF/Cloisite-30B nanocomposite for anionic and cationic dye adsorption: optimization by design-expert, kinetic, thermodynamic, and adsorption study. J Inorg Organomet Polym. 2023 Jan;33(1):138-50. doi: 10.1007/s10904-022-02471-1.

Aledresi S, Abulfadhel J, Alshaibani, Ahmed N. Enhancing the loading capacity of kojic acid dipalmitate in liposomes. Lat Am J Pharm. 2020;39(7):1333-9.

Cheng S, Wei Z, Zhiming X, Yang L, Xia F. Trace analysis and identification of 33 sulfonamides and sulfonamide potentiators in eggs by ultrahigh-performance liquid chromatography coupled with quadrupole-high-field orbitrap high-resolution mass spectrometry. Anal Methods. 2021 Oct;13(38):4452-60. doi: 10.1039/d1ay01079d, PMID 34494035.

Mahajan R, Chandragiri S, Gananadhamu S, Borse V. Analytical methodology for novel lm forming mafenide spray: assay and drug excipient compatibility study by HPLC-PDA; 2022. doi: 10.21203/rs.3.rs-2345100/v1.

Leach MK, Feng ZQ, Tuck SJ, Corey JM. Electrospinning fundamentals: optimizing solution and apparatus parameters. J Vis Exp. 2011;47(47). doi: 10.3791/2494, PMID 21304466.

Aderibigbe BA, Mhlwatika Z, Nwamadi M, Balogun MO, Matshe WMR. Synthesis, characterization and in vitro analysis of polymer-based conjugates containing dihydrofolate reductase inhibitors. J Drug Deliv Sci Technol. 2019 Apr;50:388-401. doi: 10.1016/j.jddst.2019.01.038, JDDST.2019.01.038.

Alven S, Aderibigbe BA, Balogun MO, Matshe WMR, Ray SS. Polymer-drug conjugates containing antimalarial drugs and antibiotics. J Drug Deliv Sci Technol. 2019 Oct;53:101171. doi: 10.1016/j.jddst.2019.101171.

Abbaspour M, Sharif Makhmalzadeh BS, Rezaee B, Shoja S, Ahangari Z. Evaluation of the antimicrobial effect of chitosan/polyvinyl alcohol electrospun nanofibers containing mafenide acetate. Jundishapur J Microbiol. 2015;8(10):e24239. doi: 10.5812/jjm.24239, PMID 26587214.

Mostafa M, Kandile NG, Mahmoud MK, Ibrahim HM. Synthesis and characterization of polystyrene with embedded silver nanoparticle nanofibers to utilize as antibacterial and wound healing biomaterial. Heliyon. 2022;8(1, Jan):e08772. doi: 10.1016/j.heliyon.2022.e08772, PMID 35118204.

Al-Arjan WS, Khan MUA, Almutairi HH, Alharbi SM, Razak SIA. pH-responsive PVA/BC-f-GO dressing materials for burn and chronic wound healing with curcumin release kinetics. Polymers (Basel). 2022;14(10). doi: 10.3390/polym14101949, PMID 35631834.

Gopalakrishnan S, Dathathri E, Thakur G, Koteshwara KB. Development of Polyvinlyl alcohol-chitosan-hydroxypropyl methylcellulose-based composite films for controlled drug delivery. Trends Biomater Artif Organs. 2017;31(3).

Ali M, Namjoshi S, Benson HAE, Kumeria T, Mohammed Y. Skin biomechanics: breaking the dermal barriers with microneedles. Nano Trans Med. 2022;1(1):e9130002. doi: 10.26599/NTM.2022.9130002.

IISB. In: Kharagpur IIT, editor. M, BSSC: Indian Institute of Technology (Kharagpur, I. I.E. Indian Institute of Technology, I. I. S. P. S. S. B. C. Kharagpur: Institute of Electrical and Electronics Engineers. Kharagpur Section, I. I. A. J. S. B. C (Kharagpur, and Institute of Electrical and Electronics Engineers. Women in Engineering Affinity: IEEE Students’ Technology Symposium; 2016.

Zadeh BSM, Zamin BK. The effect of chitosan coating on mafenide acetate-loaded liposome characterization and delivery through burned rat skin. v12i7.32338. Asian J Pharm Clin Res. 2019;12:212-7. doi: 10.22159/ajpcr.2019.

Mahajan R, Chandragiri S, Gananadhamu S, Borse V, Asthana A. Analytical methodology for novel film forming mafenide spray: assay and drug excipient compatibility study by HPLC-PDA”; 2022. p. 1-18. doi: 10.21203/rs.3.rs-2345100/v1.

Orachorn N, Bunkoed O. Nanohybrid magnetic composite optosensing probes for the enrichment and ultra-trace detection of mafenide and sulfisoxazole. Talanta Talanta. 2021;228:122237. doi: 10.1016/j.talanta.2021.122237, PMID 33773739.

Mahajan R, Chandragiri S, Gananadhamu S, Borse V. Analytical methodology for novel lm forming mafenide spray: assay and drug excipient compatibility study by HPLC-PDA. Research Square; 2022. doi: 10.21203/rs.3.rs-2345100/v1.

Ndunda EN. Molecularly imprinted polymers-a closer look at the control polymer used in determining the imprinting effect: a mini review. J Mol Recognit. 2020;33(11):e2855. doi: 10.1002/jmr.2855, PMID 32529728.

Jin Z, Du L, Zhang C, Sugiyama Y, Wang W, Palui G. Modification of poly (maleic anhydride)-based polymers with H2N-R nucleophiles: addition or substitution reaction? Bioconjug Chem. 2019 Mar;30(3):871-80. doi: 10.1021/acs.bioconjchem.9b00008, PMID 30759981.

Ferreira AC, Diniz MF, Babetto Ferreira AC, Sanches NB, da Costa Mattos E. FT-IR/UATR and FT-IR transmission quantitative analysis of PBT/PC blends. Polym Test. 2020;85. doi: 10.1016/j.polymertesting.2020.106447.

Santos Oliveira I, Marrote Manzano C, Hideki Nakahata D, Brentini Santiago M, Bernadelli Sousa Silva N, Henrique Gomes Martins C. Antibacterial and antifungal activities in vitro of a novel silver(I) complex with sulfadoxine-salicylaldehyde schiff base. Polyhedron. 2022;225. doi: 10.1016/j.poly.2022.116073.

Alishahi M, Khorram M, Asgari Q, Davani F, Goudarzi F, Emami A. Glucantime-loaded electrospun core-shell nanofibers composed of poly(ethylene oxide)/gelatin-poly(vinyl alcohol)/chitosan as dressing for cutaneous leishmaniasis. Int J Biol Macromol. 2020 Nov;163:288-97. doi: 10.1016/j.ijbiomac.2020.06.240, PMID 32610052.

Osman Z, Arof AK. FTIR studies of chitosan acetate-based polymer electrolytes. Electrochim Acta. 2003 Apr;48(8):993-9. doi: 10.1016/S0013-4686(02)00812-5.

Yang H, Liu H. Pyrene-functionalized silsesquioxane as fluorescent nanoporous material for antibiotics detection and removal. Micropor Mesopor Mater. 2020;300. doi: 10.1016/j.micromeso.2020.110135.

Soni R, Asoh TA, Uyama H. Cellulose nanofiber reinforced starch membrane with high mechanical strength and durability in water. Carbohydr Polym. 2020;238:116203. doi: 10.1016/j.carbpol.2020.116203, PMID 32299565.

Abraham A, Soloman PA, Rejini VO. Preparation of chitosan-polyvinyl alcohol blends and studies on thermal and mechanical properties. Procedia Technol. 2016;24:741-8. doi: 10.1016/j.protcy.2016.05.206.

Guan Y, Yu C, Zang Z, Wan X, Naeem A, Zhang R. Chitosan/xanthan gum-based (hydroxypropyl methylcellulose-co-2-Acrylamido-2-methylpropane sulfonic acid) interpenetrating hydrogels for controlled release of amorphous solid dispersion of bioactive constituents of Pueraria lobatae. Int J Biol Macromol. 2023 Jan;224:380-95. doi: 10.1016/j.ijbiomac.2022.10.131, PMID 36265534.

Spleis H, Sandmeier M, Claus V, Bernkop Schnurch A. Surface design of nanocarriers: key to more efficient oral drug delivery systems. Adv Colloid Interface Sci. 2023;313:102848. doi: 10.1016/j.cis.2023.102848, PMID 36780780.

Tan Y, Fan S, Wu X, Liu M, Dai T, Liu C. Fabrication of a three-dimensional printed gelatin/sodium alginate/Nano-attapulgite composite polymer scaffold loaded with leonurine hydrochloride and its effects on osteogenesis and vascularization. Int J Biol Macromol. 2023 Sep;249:126028. doi: 10.1016/j.ijbiomac.2023.126028, PMID 37506787.