THE EFFECTIVENESS OF VARIOUS CITRONELLA OIL NANOGEL FORMULATIONS AS A REPELLENT OF AEDES AEGYPTI MOSQUITO

AGUS SUBAGIYO; ARIF WIDYANTO; IQBAL ARDIANSYAH; FIRDAUS WULAN SAPUTRI; DHADHANG WAHYU KURNIAWAN

doi:10.22159/ijap.2024v16i2.50048

Authors

AGUS SUBAGIYO Ministry of Health Republic of Indonesia, Polytechnic of Health Semarang, Jl. Tirto Agung Pedalangan Banyumanik Semarang-50268, Indonesia
ARIF WIDYANTO Ministry of Health Republic of Indonesia, Polytechnic of Health Semarang, Jl. Tirto Agung Pedalangan Banyumanik Semarang-50268, Indonesia
IQBAL ARDIANSYAH Ministry of Health Republic of Indonesia, Polytechnic of Health Semarang, Jl. Tirto Agung Pedalangan Banyumanik Semarang-50268, Indonesia https://orcid.org/0000-0002-7289-5902
FIRDAUS WULAN SAPUTRI Ministry of Health Republic of Indonesia, Polytechnic of Health Semarang, Jl. Tirto Agung Pedalangan Banyumanik Semarang-50268, Indonesia
DHADHANG WAHYU KURNIAWAN Department of Pharmacy, Faculty of Health Sciences, Universitas Jenderal Soedirman, Jl. Dr. Suparno Kampus Unsoed Karangwangkal Purwokerto, Central Java-53123, Indonesia. Graduate School, Universitas Jenderal Soedirman, Jl. Dr. Suparno Kampus Unsoed Karangwangkal Purwokerto, Central Java-53123, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024v16i2.50048

Keywords:

Citronella oil, Nanogel, Repellent, Aedes aegypti, Mosquito

Abstract

Objective: The aim of this research is to determine the effectiveness of the repellency of various citronella oil nanogel formulations against Aedes aegypti mosquito bites applied to guinea pigs.

Methods: The Citronella oil nanogel was prepared by emulsification technique combined with gelling formation. The nanoemulsion formula consists of 6% Citronella oil, 10% propylene glycol, 6% combination of Tween 80 and Span 80 in variation to 4 formula, and deionized water until 100%. Nanoemulsions were then added to the gel base which is composed of Carbopol 940 and TEA. The nanogel formed was characterized physically and its effectiveness against Aedes aegypti mosquito using guinea pigs as an animal model. The repellent activity was determined by the amount of mosquito bites on the guinea pigs’s skin.

Results: The organoleptic test displayed that all formulas comply with the requirements, except formula 1. The formula 2, 3, and 4 have particle size below 100 nm and the polydispersity index (PDI) less than 0.5. According to the repellent assay showed that all formulas have the effectiveness much higher than without the intervention. Formula 3 has the highest effectiveness of repellent activity against Aedes aedgypti in guinea pigs’ skin. The repellent activity of Formula 3 showed a significant difference (p-value of 0.005) as compared to nanogel without Citronella oil.

Conclusion: The protective power of Citronella oil nanogel Formula 3 is the most effective as compared to the other formula.

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References

WHO. Dengue and severe dengue; 2019. Available from: https://www.who.int/news-room/questions-and-answers/item/dengue-and-severe-dengue.

Kotsakiozi P, Gloria Soria A, Caccone A, Evans B, Schama R, Martins AJ. Tracking the return of Aedes aegypti to Brazil, the major vector of the dengue, chikungunya and Zika viruses. PLOS Negl Trop Dis. 2017;11(7):e0005653. doi: 10.1371/journal.pntd.0005653, PMID 28742801.

Massad E, Amaku M, Coutinho FAB, Struchiner CJ, Burattini MN, Khan K. Estimating the probability of dengue virus introduction and secondary autochthonous cases in Europe. Sci Rep. 2018;8(1):4629. doi: 10.1038/s41598-018-22590-5, PMID 29545610.

Wilder Smith A, Quam M, Sessions O, Rocklov J, Liu-Helmersson J, Franco L. The 2012 dengue outbreak in Madeira: exploring the origins. Euro Surveill. 2014;19(8):20718. doi: 10.2807/1560-7917.es2014.19.8.20718, PMID 24602277.

Yuan B, Nishiura H. Estimating the actual importation risk of dengue virus infection among Japanese travelers. Plos One. 2018;13(6):e0198734. doi: 10.1371/journal.pone.0198734, PMID 29924819.

Fukusumi M, Arashiro T, Arima Y, Matsui T, Shimada T, Kinoshita H. Dengue sentinel traveler surveillance: monthly and yearly notification trends among Japanese travelers, 2006-2014. Plos Negl Trop Dis. 2016;10(8):e0004924. doi: 10.1371/journal.pntd.0004924, PMID 27540724.

Indonesia KKR. Profil Kesehatan Indones Tahun; 2021.

Tisgratog R, Sanguanpong U, Grieco JP, Ngoen Kluan R, Chareonviriyaphap T. Plants traditionally used as mosquito repellents and the implication for their use in vector control. Acta Trop. 2016;157:136-44. doi: 10.1016/j.actatropica.2016.01.024, PMID 26826392.

Afify A, Potter CJ. Insect repellents mediate species-specific olfactory behaviours in mosquitoes. Malar J. 2020;19(1):127. doi: 10.1186/s12936-020-03206-8, PMID 32228701.

Chen Hussey V, Behrens R, Logan JG. Assessment of methods used to determine the safety of the topical insect repellent N,N-diethyl-m-toluamide (DEET). Parasit Vectors. 2014;7(173):173. doi: 10.1186/1756-3305-7-173, PMID 24892824.

Weeks JA, Guiney PD, Nikiforov AI. Assessment of the environmental fate and ecotoxicity of N,N-diethyl-m-toluamide (DEET). Integr Environ Assess Manag. 2012;8(1):120-34. doi: 10.1002/ieam.1246, PMID 22006575.

Azeem M, Zaman T, Tahir M, Haris A, Iqbal Z, Binyameen M. Chemical composition and repellent activity of native plants essential oils against dengue mosquito, aedes aegypti. Ind Crops Prod. 2019;140. doi: 10.1016/j.indcrop.2019.111609.

Kalita B, Bora S, Sharma AK. Plant essential oil as mosquito repellent-a review. Int J Res Dev Pharm Life Sci. 2013;3(1):715-21.

Salvioni L, Morelli L, Ochoa E, Labra M, Fiandra L, Palugan L. The emerging role of nanotechnology in skincare. Adv Colloid Interface Sci. 2021;293:102437. doi: 10.1016/j.cis.2021.102437, PMID 34023566.

Gurpreet K, Singh SK. Review of nanoemulsion formulation and characterization techniques. Indian J Pharm Sci. 2018;80(5):781-9. doi: 10.4172/pharmaceutical-sciences.1000422.

Kurniawan DW, Agustina VN, Sunarto Wibowo GA, Wibowo GA, Syamsu Hidayat MZ. Formulation of cinnamon bark essential oil gel as mosquito repellent. Int J App Pharm. 2022;14(1):208-12. doi: 10.22159/ijap.2022v14i1.43034.

Kurniawan DW, Jajoriya AK, Dhawan G, Mishra D, Argemi J, Bataller R. Therapeutic inhibition of spleen tyrosine kinase in inflammatory macrophages using PLGA nanoparticles for the treatment of non-alcoholic steatohepatitis. J Control Release. 2018;288:227-38. doi: 10.1016/j.jconrel.2018.09.004, PMID 30219279.

BPOM RI. Peraturan badan pengawas obat dan makanan no 20 tahun 2023 tentang pedoman uji farmakodinamik praklinik obat tradisional. Jakarta: BPOM RI; 2023.

Subagiyo A, Widyanto A, Khomsatun Ananta IP, Ananta IP, Kurniawan DW. The effectiveness of citronella oil microemulsion as a repellent of aedes aaegypti mosquito. Int J App Pharm. 2022;14(3):56-60. doi: 10.22159/ijap.2022v14i3.44217.

Kurniawan DW, Booijink R, Pater L, Wols I, Vrynas A, Storm G. Fibroblast growth factor 2 conjugated superparamagnetic iron oxide nanoparticles (FGF2-SPIONs) ameliorate hepatic stellate cells activation in vitro and acute liver injury in vivo. J Control Release. 2020;328:640-52. doi: 10.1016/j.jconrel.2020.09.041, PMID 32979454.

Danaei M, Dehghankhold M, Ataei S, Hasanzadeh Davarani F, Javanmard R, Dokhani A. Impact of particle size and polydispersity index on the clinical applications of lipidic nanocarrier systems. Pharmaceutics. 2018;10(2). doi: 10.3390/pharmaceutics10020057, PMID 29783687.

Riana Ningsih D. Formulation of M/A-type ointment dosage from ethanol extract of white plumeria leaves (Plumeria alba l.) against candida albicans. J Pure App Chem Res. 2018;7(3):247-56. doi: 10.21776/ub.jpacr.2018.007.03.421.

Modjtahedi BS, Fortenbach CR, Marsano JG, Gandhi AM, Staab R, Maibach HI. Guinea pig sensitization assays: an experimental comparison of three methods. Cutan Ocul Toxicol. 2011;30(2):129-37. doi: 10.3109/15569527. 2010.544277, PMID 21265706.

Reinhold JM, Lazzari CR, Lahondere C. Effects of the environmental temperature on aedes aegypti and aedes albopictus mosquitoes: a review. Insects. 2018;9(4). doi: 10.3390/insects9040158, PMID 30404142.

Canyon DV, Muller R, Hii J LK. Aedes aegypti disregard humidity-related conditions with adequate nutrition. Trop Biomed. 2013;30(1):1-8. PMID 23665702.

Halim R, Lesmana O, Sitepu FY. The effect of citronella oil as antimosquito spray. Int J Mosq Res. 2021;8(5):44-7.