EFFECTIVENESS OF PROPOLIS EXTRACT GEL AS ADJUNCTIVE PERIODONTAL THERAPY: OVERVIEW OF FIBROBLASTS AND NEUTROPHILS (IN VIVO STUDY)

JULIA MAHARANI; AMINAH DALIMUNTHE; PITU WULANDARI; WITA ANGGRAINI; SYAFRUDDIN ILYAS; OLIVIA AVRIYANTI HANAFIAH

doi:10.22159/ijap.2024.v16s2.05

Authors

JULIA MAHARANI Faculty of Dentistry, Universitas Sumatera Utara, Medan, Indonesia
AMINAH DALIMUNTHE Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara
PITU WULANDARI Department of Periodontics, Faculty of Dentistry, Universitas Sumatera Utara, Medan, Indonesia https://orcid.org/0000-0001-8337-2691
WITA ANGGRAINI Department of Periodontology, Faculty of Dentistry, Universitas Trisakti
SYAFRUDDIN ILYAS Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara
OLIVIA AVRIYANTI HANAFIAH Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Sumatera Utara

DOI:

https://doi.org/10.22159/ijap.2024.v16s2.05

Keywords:

Propolis, Periodontitis, Fibroblast, Neutrophil

Abstract

Objective: Propolis has therapeutic activities contributed by its active secondary metabolites to fasten the healing of periodontitis. This study was to analyze the effectiveness of propolis extract gel as an adjunctive periodontal therapy.

Methods: The study used 50 periodontitis-induced Wistar rats, were divided into five treatment groups, and were treated by applying propolis gel with concentrations of 50%, 60%, and 70%, metronidazole gel, and placebo gel after mechanical therapy. The number of fibroblasts and neutrophils was obtained by removing the mandibular gingival tissue on the third and seventh days and were observed using a binocular microscope with 400x magnification using hematoxylin-eosin staining.

Results: The group administered with propolis extract gel showed a higher number of fibroblast cells and lower number of neutrophil cells. The 70% propolis gel appeared effective in inducing the fibroblasts and reducing the neutrophils (p<0.05).

Conclusion: Propolis extract gel can accelerate the healing of periodontitis and potentially be used as adjunctive therapy for periodontitis treatment.

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