HAIR TONIC OF BROCCOLI (BRASSICA OLERACEA VAR. ITALICA) EXTRACT: FORMULATION, PHYSICAL CHARACTERISTIC, STABILITY AND HAIR GROWTH ACTIVITY TEST

Authors

  • BENNI ISKANDAR School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei-11031, Taiwan. MD Research ltd, 25 Indescon Square London, London-110301, United Kingdom. BioMed Laboratories ltd, Cardif, Wales MTWRA-2109, United Kingdom. Department of Pharmaceutical Technology, Sekolah Tinggi Ilmu Farmasi Riau (STIFAR), Pekanbaru-28289, Indonesia
  • PRICELLA AQWILLA GINTING Department Pharmacology-Clinic, School of Pharmacy, Institut Teknologi Bandung, Bandung-40135, Indonesia. Faculty of Pharmacy and Health, Institut Kesehatan Helvetia, Medan-20124, Indonesia
  • ENDANG S. SITORUS Faculty of Pharmacy and Health, Institut Kesehatan Helvetia, Medan-20124, Indonesia
  • AKHMAD FAJRI WIDODO Institute of Injury Prevention and Control, College of Public Health, Taipei Medical University, Taipei-11031, Taiwan
  • CHING-PENG WEI School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei-11031, Taiwan. 2MD Research ltd, 25 Indescon Square London, London-110301, United Kingdom. 3BioMed Laboratories ltd, Cardif, Wales MTWRA-2109, United Kingdom
  • lENY Faculty of Pharmacy and Health, Institut Kesehatan Helvetia, Medan-20124, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024.v16s3.01

Keywords:

Hair Tonic, Broccoli (Brassica oleracea var. Italica), Hair loss

Abstract

Objective: Hair loss is a common scalp concern for both men and women. Broccoli (Brassica oleracea var. Italica) is a vegetable plant in the cabbage family or brassicaceae that has antioxidant activity and levels of flavonoids that can encourage hair growth. Hair tonic is one of cosmetic products that contain ingredients to nourish hair. The purpose of this study was to investigate the physical stability and activity of hair development in hair tonic preparations using broccoli stalks extracted at different concentrations of 30%, 35%, and 40%, followed by an examination of the hair growth activity test in an animal model.

Methods: This study hair tonic was prepared with Broccoli (Brassica oleracea var. Italica), the sample used the broccoli stalks, which was taken by purposive sampling. Evaluation of the preparation includes physical characteristic observation, pH examination, homogeneity of the preparation, irritation test, and stability test of the preparation using a cycling test, and check the activity test of hair growth in rats’ skin.

Results: The results showed that the hair tonic preparation containing broccoli stalks extracts was stable at a temperature (4 °C±40 °C) where the odor, color, shape, homogeneity, and pH remained constant. In statistical testing using ANOVA analysis, each group of the hair tonic formula of broccoli stalks extract showed hair growth activity in male white rats. The hair tonic formulation containing 40% broccoli stalks extract had better hair growth activity compared to the 30% and 35% formula groups. The hair tonic prepared from broccoli extract is confirmed safe for use after successfully passing the skin irritation test.

Conclusion: The hair tonic of broccoli extracts had met the standard of physical characteristics during storage time and it has a good activity test on hair growth in male white rats. This study provides a novel formulation in hair growth.

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References

Kwon Y, Park J, Seol H, Lee S, Ryeom GG, Heo C, Kang S. lB1680 effects of Ganoderma lucidum extract and its active component, ganoderic acid a on hair loss prevention. J Invest Dermatol. 2023;143(9):B11.

Meeker TJ, Schmid AC, Keaser ML, Khan SA, Gullapalli RP, Dorsey SG. Tonic pain alters functional connectivity of the descending pain modulatory network involving amygdala, periaqueductal gray, parabrachial nucleus and anterior cingulate cortex. Neuroimage. 2022;256:119278. doi: 10.1016/j.neuroimage.2022.119278, PMID 35523367.

Okole B, Pillai SK, Ndzotoyi P, Phasha V. Use of herbal extract-based nanoemulsions for hair care application. In: Nanotechnology for the preparation of cosmetics using plant-based extracts. Elsevier; 2022 Jan 1. p. 203-33.

Han S, Jang HS, Shim JH, Kang M, lee Y, Park JS, Kim M, Abudureyimu G, lee D, Koo H. Development of minoxidil-loaded double emulsion PLGA nanoparticles for the treatment of hair loss. Journal of Industrial and Engineering Chemistry. 2022;113:161-9.

Martins Ferreira L, Binz T, Yegles M. The influence of ethanol-containing cosmetics on ethyl glucuronide concentration in hair. Forensic Sci Int. 2012;218(1-3):123-5. doi: 10.1016/j.forsciint.2011.10.015, PMID 22051770.

Sayyad S, Midhunchakkaravarthy D, Sayyad F. Deep review on alopecia areata diagnosis for hair loss related autoimmune disorder problem. Int J App Pharm. 2022;14:8-12. doi: 10.22159/ijap.2022.v14ti.19.

Rajput M, Kumar N. Medicinal plants: a potential source of novel bioactive compounds showing antimicrobial efficacy against pathogens infecting hair and scalp. Gene Rep. 2020;21:100879. doi: 10.1016/j.genrep.2020.100879.

Norooznezhad AH, Yarani R, Payandeh M, Hoseinkhani Z, Mahmoudi H, Kiani S. Treatment of persistent chemotherapy-induced hair loss (alopecia) with human mesenchymal stromal cells exosome enriched extracellular vesicles: a case report. Heliyon. 2023;9(4):e15165. doi: 10.1016/j.heliyon.2023.e15165, PMID 37095978.

Kumar V, Charde V, Prasad SB, Gandhi Y, Mishra SK, Rawat H. Therapeutic potential of evergreen maiden hair fern Adiantum venustum D. don: a comprehensive review. Food Chem Adv. 2023 Dec 1;3:100439. doi: 10.1016/j.focha.2023.100439.

Makhlouf A, Elnawawy T. Hair regrowth boosting via minoxidil cubosomes: formulation development, in vivo hair regrowth evaluation, histopathological examination and confocal laser microscopy imaging. Int J Pharm. 2023;634:122665. doi: 10.1016/j.ijpharm.2023.122665, PMID 36736676.

Sharannavar B, Amonkar MB, Inamdar P, Kulkarni M. Formulation and evaluation of a topical gel containing minoxidil and tofacitinib citrate for alopecia areata. Int J App Pharm. 2023;15(5):75-83. doi: 10.22159/ijap.2023v15i5.45798.

Korassa YB, Saptarini NM, Mustarichie R, Hendriani R, Ola AR, Novicadlitha Y. Kapitan lAV. Anti-alopecia activity of moringa (Moringa oleifera lamk.) seed oil against dihydrotestosterone-induced rabbits. Int J Appl Pharm. 2023;15(2):19-24.

Hussein RS, Dayel SB, Abahussein O. Botulinum toxin a for hair loss treatment: a systematic review of efficacy, safety, and future directions. JPRAS Open. 2023;38:296-304. doi: 10.1016/j.jpra.2023.09.006, PMID 38021319.

Wu Y, Zhang J, liu Q, Miao Z, Chai R, Chen W. Development of Chinese herbal medicine for sensorineural hearing loss. Acta Pharm Sin B. 2024;14(2):455-67. doi: 10.1016/j.apsb.2023.11.001, PMID 38322328.

Arndt T, Schrofel S, Stemmerich K. Ethyl glucuronide identified in commercial hair tonics. Forensic Sci Int. 2013;231(1-3):195-8. doi: 10.1016/j.forsciint.2013.05.010, PMID 23890636.

Iraqui P, Das MK. Herbal cosmeceuticals for beauty and skin therapy. In: Nanocosmeceuticals. Academic Press; 2022 Jan 1. p. 453-80.

Chakraborty A, bhattacharjee A, chakraborty M, Mukhopadhyay G. Process validation of beta-sitosterol hair gel formulation and evaluation of 5 alpha reductase inhibition in vitro for the treatment of androgenetic alopecia. Int J App Pharm. 2023;15(2):146-52. doi: 10.22159/ijap.2023v15i2.46757.

Tinoco A, Martins M, Cavaco Paulo A, Ribeiro A. Biotechnology of functional proteins and peptides for hair cosmetic formulations. Trends Biotechnol. 2022;40(5):591-605. doi: 10.1016/j.tibtech.2021.09.010, PMID 34666897.

Pereira Silva M, Martins AM, Sousa Oliveira I, Ribeiro HM, Veiga F, Marto J. Nanomaterials in hair care and treatment. Acta Biomater. 2022;142:14-35. doi: 10.1016/j.actbio.2022.02.025, PMID 35202853.

Rosalina AI, Iskandarsyah E, Sagita E. Placenta extract-loaded novasome significantly improved hair growth in a rat in vivo model. Int J App Pharm. 2023;15(3):138-45. doi: 10.22159/ijap.2023v15i3.47459.

Ali E, Owais R, Sheikh A, Shaikh A. Olumniant (Baricitinib) oral tablets: an insight into FDA-approved systemic treatment for alopecia areata. Ann Med Surg (Lond). 2022;80:104157. doi: 10.1016/j.amsu.2022.104157, PMID 36045780.

Li H, Xia Y, Liu HY, Guo H, He XQ, Liu Y. Nutritional values, beneficial effects, and food applications of broccoli (Brassica oleracea var. italica Plenck). Trends Food Sci Technol. 2022;119:288-308. doi: 10.1016/j.tifs.2021.12.015.

Pacheco Cano RD, Salcedo Hernandez R, Lopez Meza JE, Bideshi DK, Barboza‐Corona JE. Antimicrobial activity of broccoli (Brassica oleracea var. italica) cultivar Avenger against pathogenic bacteria, phytopathogenic filamentous fungi and yeast. J Appl Microbiol. 2018;124(1):126-35. doi: 10.1111/jam.13629, PMID 29112318.

Makky AM, El-Leithy ES, Hussein DG, Khattab A. Skin targeting of an optimized caffeine nanostructured lipid carrier with improved efficiency against chemotherapy-induced alopecia. Int J App Pharm. 2022;14(4):235-50. doi: 10.22159/ijap.2022v14i4.44681.

Li Z, Mei Y, liu Y, Fang Z, Yang L, Zhuang M. The evolution of genetic diversity of broccoli cultivars in China since 1980. Sci Hortic. 2019 May 10;250:69-80. doi: 10.1016/j.scienta.2019.02.034.

Villano D, Lopez Chillon MT, Zafrilla P, Moreno DA. Bioavailability of broccoli sprouts in different human overweight populations. J Funct Foods. 2019;59:337-44. doi: 10.1016/j.jff.2019.05.052.

Szymandera Buszka K, Piechocka J, Zaremba A, Przeor M, Jędrusek Golinska A. Pumpkin, cauliflower and broccoli as new carriers of thiamine compounds for food fortification. Foods. 2021;10(3):578. doi: 10.3390/foods10030578, PMID 33801931.

Petkowicz CL, Williams PA. Pectins from food waste: characterization and functional properties of a pectin extracted from broccoli stalk. Food Hydrocoll. 2020;107:105930. doi: 10.1016/j.foodhyd.2020.105930.

Radosevic K, Srcek VG, Bubalo MC, Rimac Brncic SR, Takacs K, Redovnikovic IR. Assessment of glucosinolates, antioxidative and antiproliferative activity of broccoli and collard extracts. J Food Compos Anal. 2017;61:59-66. doi: 10.1016/j.jfca.2017.02.001.

Traka MH, Melchini A, Coode Bate J, Al Kadhi O, Saha S, Defernez M. Transcriptional changes in prostate of men on active surveillance after a 12-mo glucoraphanin-rich broccoli intervention-results from the effect of sulforaphane on prostate cancer prevention (ESCAPE) randomized controlled trial. Am J Clin Nutr. 2019;109(4):1133-44. doi: 10.1093/ajcn/nqz012, PMID 30982861.

Gonzalez Hidalgo I, Moreno DA, Garcia Viguera C, Ros Garcia JM. Effect of industrial freezing on the physical and nutritional quality traits in broccoli. Food Sci Technol Int. 2019;25(1):56-65. doi: 10.1177/1082013218795807, PMID 30153746.

Rahmi IA, Mun’im A, Jufri M. Formulation and evaluation of phytosome lotion from Nothopanax scutellarium leaf extract for hair growth. Int J App Pharm. 2021;13(6):178-85. doi: 10.22159/ijap.2021v13i6.42169.

Aranaz P, Navarro Herrera D, Romo Hualde A, Zabala M, Lopez Yoldi M, Gonzalez Ferrero C. Broccoli extract improves high fat diet-induced obesity, hepatic steatosis and glucose intolerance in wistar rats. Journal of Functional Foods. 2019;59:319-28. doi: 10.1016/j.jff.2019.05.054.

De Sousa Vaz AF, dos Santos Ferreira T, Dos Santos DM, Correia ME. Millicomposting: sustainable technique for obtaining organic compost for the cultivation of broccoli seedlings. Cleaner Engineering and Technology. 2022;7:100442.

Uronnachi E, Atuegwu C, Umeyor C, Nwakile C, Obasi J, Ikeotuonye C. Formulation and evaluation of hair growth enhancing effects of oleogels made from rosemary and cedar wood oils. Sci Afr. 2022;16:e01223. doi: 10.1016/j.sciaf.2022.e01223.

Gupta A, Malviya R, Singh TP, Sharma PK. Indian medicinal plants used in hair care cosmetics: a short review. Pharmacogn J. 2010;2(10):361-4. doi: 10.1016/S0975-3575(10)80110-5.

Raeeszadeh M, Shokrollahi B, Khademi N, Akbari A. Superior effect of broccoli methanolic extract on control of oxidative damage of sperm cryopreservation and reproductive performance in rats: a comparison with vitamin C and E antioxidant. Theriogenology. 2022;181:50-8. doi: 10.1016/j.theriogenology.2022.01.010, PMID 35063921.

Guo K, Wang L, Mahe J, Li L, Jiao S, Wang H. Effect of aqueous extract of seed of broccoli on inflammatory cytokines and Helicobacter pylori infection: a randomized, double-blind, controlled trial in patients without atrophic gastritis. Inflammopharmacology. 2022;30(5):1659-68. doi: 10.1007/s10787-022-01030-x, PMID 35831736.

Fang T, Xu R, Sun S, He Y, Yan Y, Fu H. Caizhixuan hair tonic regulates both apoptosis and the PI3K/Akt pathway to treat androgenetic alopecia. Plos One. 2023;18(2):e0282427. doi: 10.1371/journal.pone.0282427, PMID 36827412.

Rachitha P, Krishnaswamy K, lazar RA, Gupta VK, Inbaraj BS, Raghavendra VB. Attenuation of hyperlipidemia by medicinal formulations of Emblica officinalis synergized with nanotechnological approaches. Bioengineering (Basel). 2023;10(1):64. doi: 10.3390/bioengineering10010064, PMID 36671636.

Iskandar B, Karsono SJ. Preparation of spray nanoemulsion and cream containing vitamin E as antiaging product tested in vitro and in vivo method. Int J Pharmtech Res. 2016;9(6):307-8.

Iskandar B, Novita G, Annisa FF, Hafiz I, Surboyo MD, Lee CK. Evaluation of physical quality and antioxidant activity of ethanol extract of moringa leaves (Moringa oleifera lAM) formulated in emulgel preparation. Res J Pharm Technol. 2022;15(6):2703-8.

Kongkon P, Pichayakorn W, Sanohkan S. Evaluation of in vivo bond strength and skin irritation test for new skin adhesive. J Oral Biol Craniofac Res. 2023;13(6):731-8. doi: 10.1016/j.jobcr.2023.10.001, PMID 37915313.

Hashim N, Abdullah S, Hassan LS, Mohamed RM, Mohamed A. Antimicrobial ability and free-irritation effect of neem-based lotion cosmeceutical for skin care. Mater Today Proc. 2023 Feb 6.

Iskandar B, Putri RS, Novita G, Surboyo MD, Lee CK. Formulation and activity test of sunflower oil (Helianthus annuus l.) liquid soap as anti acne. Int J Appl Pharm. 2022;14Special Issue 3:55-9.

Tsujimoto H, Hara K, Tsukada Y, Huang CC, Kawashima Y, Arakaki M. Evaluation of the permeability of hair growing ingredient encapsulated PLGA nanospheres to hair follicles and their hair growing effects. Bioorg Med Chem Lett. 2007;17(17):4771-7. doi: 10.1016/j.bmcl.2007.06.057, PMID 17658251.

Iskandar B, lukman A, Syaputra S, Al-Abrori UN, Surboyo MD, Lee CK. Formulation, characteristics and antibacterial effects of Euphorbia hirta l. mouthwash. J Taibah Univ Med Sci. 2022;17(2):271-82. doi: 10.1016/j.jtumed.2021.08.009, PMID 35592805.

Shruthi PA, Pushpadass HA, Magdaline Eljeeva Emerald F, Surendra Nath B, Laxmana Naik N. Formulation and characterization of catechin-loaded proniosomes for food fortification. J Sci Food Agric. 2021;101(6):2439-48. doi: 10.1002/jsfa.10868, PMID 33027542.

Iskandar B, Mei HC, Liu TW, Lin HM, Lee CK. Evaluating the effects of surfactant types on the properties and stability of oil-in-water Rhodiola rosea nanoemulsion. Colloids Surf B Biointerfaces. 2024;234:113692. doi: 10.1016/j.colsurfb.2023.113692, PMID 38104466.

Utami SM, Djajadisastra J, Saputri FC. Using hair growth activity, physical stability, and safety tests to study hair tonics containing ethanol extract of licorice (Glycyrrhiza glabra linn.). Int J App Pharm. 2017;9:44-8. doi: 10.22159/ijap.2017.v9s1.20_25.

Calabrese EJ. Stimulating hair growth via hormesis: experimental foundations and clinical implications. Pharmacol Res. 2020;152:104599. doi: 10.1016/j.phrs.2019.104599, PMID 31857242.

Published

12-08-2024

How to Cite

ISKANDAR, B., GINTING, P. A. ., SITORUS, E. S., WIDODO, A. F., WEI, C.-P., & lENY. (2024). HAIR TONIC OF BROCCOLI (BRASSICA OLERACEA VAR. ITALICA) EXTRACT: FORMULATION, PHYSICAL CHARACTERISTIC, STABILITY AND HAIR GROWTH ACTIVITY TEST. International Journal of Applied Pharmaceutics, 16(3), 1–8. https://doi.org/10.22159/ijap.2024.v16s3.01

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