ASSESSMENT OF ANTIDIABETIC POTENTIAL OF TRADITIONAL MEDICINAL PLANTS IN HUMAN WHOLE BLOOD SAMPLES
Abstract
ABSTRACT
Introduction: Evaluation of antidiabetic studies was conducted on different medicinal plant products (Trigonella foenum-graecum, seeds; Syzygium
cumini, seeds; Salavadora persica, leaves; and Terminalia chebula, seeds) on human whole blood samples. Since centuries revealed the presence of
potent antidiabetic activity in primary and secondary metabolites of medicinal plant products.
Objective: The objective of our study is to screen these plant extracts of four different medicinal plant products on diabetic human whole blood samples.
Methods: In this study, we screened plant extracts pertaining to determine its secondary metabolites qualitatively and also analyzed its activity on
diabetic human whole blood samples to determine total cellular content, free hemoglobin in the supernatant and also estimated its glucose content.
Results: The results of these studies claimed that these plant extracts showed antidiabetic effect at lower doses because of decline in total cellular
content, free hemoglobin in the supernatant, and glucose content. Overall, this study claimed that all these plant extracts showed antidiabetic activity.
Conclusion: Overall, this study claimed that all these plant extracts showed antidiabetic activity.
Keywords: Trigonella foenum-graecum, Syzygium cumini, Salavadora persica, Terminalia chebula, Antidiabetic.
References
REFERENCES
Gupta M, Singh R, Lehl SS. Diabetes in India: A long way to go. Int J
Sci Rep 2015;1(1):1-2.
Baruah MP, Pathak A, Kalra S, Das AK, Zargar AH, Bajaj S, et al.
A revisit to prevailing care and challenges of managing diabetes
in India: Focus on regional disparities. Indian J Endocrinol Metab
;18(3):254-63.
Patel DK, Prasad SK, Kumar R, Hemalatha S. An overview on
antidiabetic medicinal plants having insulin mimetic property. Asian
Pac J Trop Biomed 2012;2(4):320-30.
Deutschländer MS, van de Venter M, Roux S, Louw J, Lall N.
Hypoglycaemic activity of four plant extracts traditionally used in
South Africa for diabetes. J Ethnopharmacol 2009;124(3):619-24.
Kumari L, Mazumder PM. HPTLC finger printing profile and
evaluation of in-vitro antioxidant activity of extracts from seeds of
Trigonella foneum graecum Linn in different solvents. Int J Res Pharm
Biomed Sci 2013;4(2):417-27.
Subhashini N, Thangathirupathi A, Lavanya N. Antioxidant activity of
Trigonella foenum graecum using various in vitro and ex vivo models.
Int J Pharm pharm Sci 2011;3(2):96-102.
Bansode TS, Gupta A, Chaphalkar SR, Salalkar BK. Integrating in-silico
and in-vitro approaches to screen the antidiabetic drug from Trigonella
foenum graecum Linn. Int J Biochem Res Rev 2016;14(3):1-10.
Sreeja S, Anju VS, Sreeja S. In vitro estrogenic activities of fenugreek
Trigonella foenum graecum seeds. Indian J Med Res 2010;131:814-9.
Gupta A, Chaphalkar SR. Flow cytometry based assay of formulation
from Syzygium cumini in human whole blood and glycosylated red
blood cells. J Pharm Res 2014;3(12):265-70.
Prince P, Venon M. Effect of Syzigium cumini in plasma antioxidants
Innovare Journal of Health Sciences, Vol 5, Issue 2, 2017, 1-4
Bansode et al.
on alloxan-induced diabetes in rats. J Clin Biochem Nutr
;25(2):81-6.
Bansode TS, Gupta A, Salalkar BK. In silico and in vitro assessment
on antidiabetic efficacy of secondary metabolites from Syzygium
cumini (L.) Skeels. Plant Sci Today 2016;3(4):360-7.
Sukkarwalla A, Ali SM, Lundberg P, Tanwir F. Efficacy of miswak on
oral pathogens. Dent Res J 2013;10(3):314-20.
Bansode TS, Salalkar BK. Exploiting the therapeutic potential of
secondary metabolites from Salvadora persica for diabetes using
in silico and in vitro approach. J Life Sci Biotechnol 2016;5:127-36.
Bag A, Bhattacharyya SK, Chattopadhyay RR. The development of
Terminalia chebula Retz. (Combretaceae) in clinical research. Asian
Pac J Trop Biomed 2013;3(3):244-52.
Tamhane MD, Thorat SP, Rege NN, Dahanukar SA. Effect of oral
administration of Terminalia chebula on gastric emptying: An
experimental study. J Postgrad Med 1997;43(1):12-3.
Afshari AR, Sadeghnia HR, Mollazadeh H. A review on potential
mechanisms of Terminalia chebula in Alzheimer’s disease. Adv
Pharmacol Sci 2016;2016:8964849.
Belapurkar P, Goyal P, Tiwari-Barua P. Immunomodulatory effects of
triphala and its individual constituents: A review. Indian J Pharm Sci
;76:467-75.
Kumar A. A review of traditional anticancer nano-medicine: Triphala.
Pharm Innov J 2014;3(7):60-6.
Gupta R, Gupta A, Singh RL. Hepatoprotective activities of Triphala
and its constituents. Int J Pharm Res Rev 2015;4:34-55.
Shaikh AC, Gupta A, Chaphalkar SR. Quantitative phytochemical
credential of Nakshtra plants and distribution analysis of aldehydes
pertaining to LC-MS. Int J Chem Pharm Sci 2016;4(8):408-15.
Gupta A, Chaphalkar SR. Haemolytic activities and anti-diabetic effect
of Terminalia arjuna and Emblica officinalis. Eur J Pharm Med Res
;3(6):334-8.
Singab AN, Youssef FS, Ashour ML. Medicinal plants with potential
antidiabetic activity and their assessment. Med Aromatic Plants
;3(1):1-12.
Mamun-or-Rashid AN, Hossain MS, Hassan BN, Dash MK,
Sapon A, Sen MK. A review on medicinal plants with antidiabetic
activity. J Pharmacogn Phytochem 2014;3(4):149-59.
Hassan Z, Yam MF, Ahmad M, Yusof AP. Antidiabetic properties
and mechanism of action of Gynura procumbens water extract in
streptozotocin-induced diabetic rats. Molecules 2010;15(12):9008-23.
Eid HM, Haddad PS. Mechanisms of action of indigenous antidiabetic
plants from the boreal forest of Northeastern Canada. Adv