DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC DETERMINATION OF CEFPODOXIME PROXETIL IN PURE AND TABLET DOSAGE FORMS THROUGH ION-PAIR COMPLEX FORMATION USING BROMOTHYMOL BLUE

Authors

  • Abdul Aziz Ramadan Department of Chemistry, Faculty of Science, University of Aleppo, Syria
  • Hasna Mandil
  • Rasha Shamseh

Keywords:

Direct spectrophotometric method, Cefpodoxime proxetil, Bromothymol blue, Ion-pair complex

Abstract

Objective: A simple, direct and accurate spectrophotometric method has been developed for the determination of cefpodoxime proxetil (CEFP) in pure and pharmaceutical formulations by complex formation with bromothymol blue (BTB).

Methods: The method involves the formation of yellow ion-pair complexes between BTB reagent and CEFP in chloroform. The two formed complexes ([CEFP]: [BTB] and [CEFP]: [BTB]2) have maximum absorption at lmax 422 nm. The proposed method was validated for specificity, linearity, precision and accuracy, repeatability, sensitivity (LOD and LOQ) and robustness with an average recovery of 99.0-101.4%.

Results: The formed complex ([CEFP]: [BTB]2) was measured against the reagent blank prepared in the same manner. Variables were studied in order to optimize the reaction conditions. Molar absorptivity (e) for two complexes were 8100 and 12600  L. mol-1. cm-1, respectively. Beer's law was obeyed in the concentration range of 0.5576-55.760 μg/ml in the present of 1x10-3 mol/l of BTB with good correlation coefficient (R2= 0.9995). The relative standard deviation did not exceed 4.7%. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.088 and 0.27 μg/ml, respectively.

Conclusion: The developed method is applicable for the determination of CEFP in pure and different dosage forms with the average assay of marketed formulations 99.5 to 103.2%, and the results are in good agreement with those obtained by the RP-HPLC reference method.  

Downloads

Download data is not yet available.

References

O' Neil MJ. Editor. The Merck Index–an Encyclopedia of Chemicals, Drugs, and Biologicals. New Jersey: Merk and Co; 2001.

British pharmacopoeia. The Department of Health. Vol. I. London: The Stationary office; 2009.

The United States Pharmacopoeia. XIX Revision. Rockville MD: United States Pharmacopoeial Convention; 1974.

Meyer D, Thierry. Substituent effects on absorption spectra of pH indicators: An experimental and computational study of sulfonphthaleins dyes. Dyes Pigments 2014;102:241–50.

Rahman N, Najmul S, Azmi H. Extractive spectrophotometric methods for determination of diltiazem HCl in pharmaceutical formulations using bromothymol blue, bromophenol blue and bromocresol green. J Pharm Biomed Anal 2000;24:33–41.

Swamy S, Shetty SK, kumar A. UV-Visible spectrophotometric methods for the estimation of cefpodoxime proxetil in bulk drug and pharmaceutical dosage form. Int J PharmTech Res 2012;4:750-6.

Srinivasa Rao Y, Chowdary KPR, Seshagiri Rao JVLN. New spectrophotometric method for determination of cefpodoxime proxetil. Chem Anal 2004;49:111.

El-Walily AF, Gazy A, Belal S, Khamis E. Quantitative determination of some thiazole cephalosporins through complexation with palladium (II) chloride. J Pharm Biomed Anal 2000;22:385–92.

Rageh A, El-Shaboury S, Saleh G, Mohamed F. Specto-photometric method for determination of certain cephalosporins using 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl). Nat Sci 2010;2:828-40.

Rao JVLNS, Rao MRP, Reddy YSN. Determination of cefpodoxime proxetil using 1,10-phenanthroline. Indian J Pharm Sci 2000;62:318-9.

Maheshwari ML, Mughal UR, Ghoto MA, Dayo A, Memon N, Arain M, et al. Spectrophotometric determination of Cefpodoxime using 2-hydroxynaphthaldehyde as a derivatizing reagent. Int J Pharm 2014;4:63-8.

Patel SM, Mehta MR, Dave JB, Patel CN. Spectrophotometric methods for simultaneous estimation of cefpodoxime proxetil and ambroxol hydrochloride in tablet dosage form. Int J Pharm Res Bio-Sci 2012;3:195-203.

Sanket P, Satish P. Simultaneous spectrophotometric determination of cefpodoxime proxetil and ofloxacin in tablets. J Appl Pharm Sci 2011;1:141-4.

Shah MN, Patel HU, Patel CN. Development and validation of spectrophotometric method for simultaneous determination of cefpodoxime proxetil and ofloxacin in tablets. Int J Pharm Sci Res 2012;38:551-5.

Jagatap CA, Patil PB, Kane SR, Mohite SK, Magdum CS. Development and validation of simultaneous spectro-photometric estimation of cefpodoxime proxetil and ofloxacin in tablet dosage form. J Pharm Res 2012;5:3181.

Sanket P, Satish P. Dual wavelength spectrophotometric method for simultaneous estimation of ofloxacin and cefpodoxime proxetil in tablet dosage form. Asian J Pharm Life Sci 2011;1:261-8.

Patil V, Chaudari RY. Spectrophotometric method for estimation of cefpodoxime proxetil and ofloxacin in tablet dosage form by simultaneous equation method. Int J Pharm Life Sci 2012;3:1982-4.

Tamilarasi G, Vetrichelvan T, Vekappayya D, Tharabai R, Yuvarajan K. Spectrophotometric estimation of levofloxacin hemihydrate and cefpodoxime proxetil in a tablet. Int J Pharm Pharm Sci 2014;6:646-8.

Gandhi SV, Patil UP, Patil NG. Simultaneous spectrophotometric determination of cefpodoxime proxetil and potassium clavulanate. Hind Antibiot Bull 2009;51:24-8.

Srinivasa Rao Y, Rajani Kumar V, Seshagiri Rao JVLN. Spectrophotometric determination of cefpodoxime proxetil. Asian J Chem 2002;14:1788-90.

Bushra U, Islam KR, Hossain S, Sarah AH, Allied AH. Method development and validation of cefpodoxime proxetil in bulk and pharmaceutical formulation by using uv spectro-photometer. Am J PharmTech Res 2014;4:818-24.

Subbayamma AV, Rambabu C. Application of ninhydrin and ascorbic acid for the determination of cefpodoxime proxetil in pharmaceutical formulations. Orient J Chem 2008;24:651-4.

Asnani G, Jadhav K, Dhamecha D, Sankh A, Patil M. Development and validation of the spectrophotometric method of cefpodoxime proxetil using hydrotropic solubilizing agents. Pharm Methods 2012;3:117–20.

Saleh G, Askal H, Darwish I, El-Shorbagi AN. Spectroscopic analytical study for the charge-transfer complexation of certain cephalosporins with chloranilic acid. Anal Sci 2003;19:281-7.

Saha C, Sreelatha G, Nazeeruddin MA. Spectrophotometric estimation of rosuvastatin calcium in bulk and pharmaceutical formulations. Int J Pharm Anal Res 2013;3:84-8.

Kumaraswamy G, Hamza Z, Suthakaran R. Development and validation of RP-HPLC for simultaneous estimation of cefpodoxime proxetil and dicloxacillin sodium tablets. Asian J Pharm Anal 2014;4:151-5.

ICH: Proceedings of the International Conference on Harmonization of Technical Requirement of Registration of Pharmaceuticals for Human Use (ICH Harmonized Tripartite Guidelines); 2000.

Published

01-08-2016

How to Cite

Ramadan, A. A., H. Mandil, and R. Shamseh. “DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC DETERMINATION OF CEFPODOXIME PROXETIL IN PURE AND TABLET DOSAGE FORMS THROUGH ION-PAIR COMPLEX FORMATION USING BROMOTHYMOL BLUE”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 8, Aug. 2016, pp. 258-63, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/12424.

Issue

Section

Original Article(s)

Most read articles by the same author(s)