SCREENING OF ANTIOXIDANT ACTIVITY AND TOTAL PHENOLIC CONTENT IN RAPHANUS SATIVUS POD
Keywords:
Raphanus sativus L Var caudatus Alef, DPPH, FRAP, Total phenolic contentAbstract
Objective: The aim of this study was to evaluate the antioxidant activity and total phenolic content of Raphanus sativus L. Var. caudatus Alef pod extracts.
Methods: In this study, the extract of R. sativus pod was prepared in three different types of solvent. The antioxidant activitiy of R. sativus extract was determined using a spectrophotometric technique, based on a free radical diphenyl-picryhydrazyl scavenging assay (DPPH assay) and a ferric reducing antioxidant power assay (FRAP assay). Total phenolic content was also observed using the spectrophotometric technique.
Results: The result showed that the antioxidant activity which was expressed by IC50 values varied from 1,365 to 4,371 mg/ml and 312 to 6,478 mg/ml, based on DPPH assay and FRAP assay, respectively. Total phenolic content was also evaluated and calculated as gallic acid equivalents which ranged from 0.26 to 34.60 mg gallic acid per 100 g fresh sample.
Conclusion: It was suggested that hexane extract of R. sativus pod contained the highest amount of phenolic compounds in comparison with those of dichloromethane part and ethanol part. The result from FRAP assay was positively correlated to total phenolic content which the highest antioxidant value belongs to the hexane extract of R. sativus pod. It was concluded that R. sativus pod contained phenolic compounds which showed mild antioxidant activity.
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