DEVELOPMENT AND VALIDATION OF LC-MS METHOD FOR QUANTITATIVE ANALYSIS OF A TRADITIONAL THAI ANTIHYPERTENSIVE HERBAL RECIPE

Authors

  • Tossaton Charoonratana Department of Pharmacognosy, Faculty of Pharmacy, Rangsit University, Lak-Hok, Muang, Pathum Thani, 12000, Thailand
  • Kingkan Bunluepuech Faculty of Traditional Thai Medicine, Prince of Songkla University, Hatyai, Songkhla, 90112, Thailand
  • Tun Chusut Sino-Thai Traditional Medicine Research Center, Faculty of Pharmacy, Rangsit University, Lak-Hok, Muang, Pathum Thani, 12000, Thailand.
  • Jirapornchai Suksaeree Sino-Thai Traditional Medicine Research Center, Faculty of Pharmacy, Rangsit University, Lak-Hok, Muang, Pathum Thani, 12000, Thailand
  • Thanapat Songsak Department of Pharmacognosy, Faculty of Pharmacy, Rangsit University, Lak-Hok, Muang, Pathum Thani, 12000, Thailand

Keywords:

Traditional Thai antihypertensive herbal recipe, LC-MS, Method validation

Abstract

Objective: To develop a new validated LC-MS method in which can be used for identification and quantification of the active pharmaceutical compounds in a traditional Thai antihypertensive herbal recipe (TTAH).

Methods:  First, a quadrupole-ion trap MS was used to identify the active pharmaceutical compounds to facilitate a set of markers in the TTAH using multiple reactions monitoring (MRM) method. Coupling to LC in gradients mode, the chromatogram of the TTAH was established. Second, the method validation was conducted to observe several parameters, such as, carryover, linearity, a limit of detection, a limit of quantification, precision, accuracy, robustness, specificity, and system suitability.

Results: Piperine, imperatorin, and pinostrobin were identified using the quadrupole-ion trap MS by comparison the mass spectrum of the TTAH samples and reference standards. The parent and product ions were optimized using MRM method. Good chromatographic peaks were achieved along with a simple and fast analysis. All parameters were validated and found that the method reported in this article can be used to quantify the amount of piperine, imperatorin, and pinostrobin in the TTAH.

Conclusion: Piperine, imperatorin, and pinostrobin were selected as the markers in LC-MS analysis of the TTAH. This new validated LC-MS method is readily to use in a quality assurance of the TTAH.

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References

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Charoonratana T, Songsak T, Monton C, Saingam W, Bunluepuech K, Suksaeree J. Quantitative analysis and formulation development of a traditional Thai antihypertensive herbal recipe. J Phytochem Rev 2014;13(2):511-24.

Published

31-08-2014

How to Cite

Charoonratana, T., K. Bunluepuech, T. Chusut, J. Suksaeree, and T. Songsak. “DEVELOPMENT AND VALIDATION OF LC-MS METHOD FOR QUANTITATIVE ANALYSIS OF A TRADITIONAL THAI ANTIHYPERTENSIVE HERBAL RECIPE”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 8, Aug. 2014, pp. 259-62, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/2774.

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