ANTIDIABETIC AND ANTIHYPERLIPIDAEMIC ACTIVITY OF NELUMBO NUCIFERA GAERTN ETHANOL SEED EXTRACT IN STREPTOZOTOCIN INDUCED DIABETIC RATS
DOI:
https://doi.org/10.22159/ijpps.2017v9i10.21455Keywords:
Nelumbo nucifera, Antidiabetic activity, Streptozotocin, Serum lipid profile, Antioxidant enzymesAbstract
Objective: The main objective of the study was to investigate the effect of ethanol seed extracts of Nelumbo nucifera Gaertn (Nymphaeaceae) as antihyperglycemic, anti-hyperlipidemic and antioxidant activity in streptozotocin-induced diabetic rats.
Methods: Diabetes was induced in wistar albino rats by administration of streptozotocin (single intraperitoneal dose of 60 mg/kg B. W). The ethanol extract of N. nucifera seed at a dose of 200 and 400 mg/kg body weight was administrated at a single dose per day to STZ induced diabetic rats for a period of 42 d. The different pharmacological parameters were evaluated. The effect of ethanol seed extract of N. nucifera on insulin, blood glucose, urea, creatinine, HbA1C, serum protein, albumin, globulin, serum enzymes, serum lipid profiles, lipid peroxides (LPO) and other antioxidant enzymes like catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) and reduced glutathione (GSH) were measured in the diabetic rats.
Results: In the acute toxicity study ethanol seed extract of Nelumbo nucifera were non-toxic at 2000 mg/kg in rats. The ethanol extract of Nelumbo nucifera seed showed significant reduction in blood glucose (p<0.05), serum enzymes (SGPT, SGOT, ALP) (p<0.05), lipid parameters (TC, TG, VLDL, LDL) (p<0.05) except HDL and significantly increased insulin (p<0.05), HDL (p<0.05), GPx, GSH, SOD and CAT (p<0.05) at the dose of 400 mg/kg when compared with the diabetic-induced control.
Conclusion: The present study suggested that the NNSE has significant (p<0.05) antihyperglycemic, antihyperlipidemic and antioxidant activity in STZ induced diabetic rats. These results clearly indicate that Nelumbo nucifera is effective against free radical-mediated diseases, thus replacing the synthetic ones.
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Kumar S, Kumar V, Prakash O. Antidiabetic and hypolipidemic activities of Dillenia indica extract in diabetic rats. Zhong Xi Yi Jie He Xue Bao 2011;9(5):570-74.
Ananda PK, Kumarappan CT, Christudas S, Kalaichelvan VK. Effect of Biophytum sensitivum on streptozotocin and nicotinamide induced diabetic rats. Asian Pac J Trop Biomed 2012;2(1):31-35.
Bandawane D, Juvekar A, Juvekar M. The Antidiabetic and antihyperlipidemic effect of Alstonia scholaris Linn bark in streptozotocin Induced diabetic rats. Indian J Pharm Educ Res 2011;45(2):114-20.
Kumar S, Malhotra R, Kumar D. Antidiabetic and free radical scavenging potential of Euphorbia hirta flower extract. Indian J Pharm Sci 2010;72(4):531-33.
Ramachandran V, Mandal D, Payyavala U, Sangai PD, Muthureddy NS, Shanish A et al. Hypoglycemic, antioxidant and hypolipidemic activity of Asparagus racemosus on streptozotocin induced diabetic rats. Adv Appl Sci Res 2011;2(3):179-85.
Kumar S, Rashmi Kumar D. Evaluation of the antidiabetic activity of Euphorbia hirta Linn. in streptozotocin induced diabetic mice. Indian J Nat Prod Resour 2010;1(2):200-203.
Shen MJ, Schopf JW, Harbottle G, Cao RJ, Ouyang S, Zhou KS et. Long-living lotus: Germination and soil irradiation of centuries-old fruits, and cultivation, growth, and phenotypic abnormalities of offspring. American J Botany 2002;89(2):236-47.
Jangra M, Sharma S, Kumar M. Evaluation of antihyperglycemic activity of Dodonaea viscosa leaves in normal and STZ diabetic rats. Int J Pharm Pharm Sci 2011;3(3):69-74.
Manimekalai P, Krishnaraju V, Davidraj C, Ssudhakar B, Dhanalakshmi R, Kalpana K. The effect of a hydro alcoholic extract of Nelumbo nucifera (G) on STZ induced diabetic rats. Int J Pharm World Res 2010;1(3):1-16.
Vessal M, Hemmati M, Vasei M. Antidiabetic effects of quercetin in streptozotocin induced diabetic rats.Comparative Biochem & Physio 2003;3:357-64.
Anderson L, Dinesen B, Jorgonsen PN, Poulsen F, Roder MF. Enzyme immune assay for intact human insulin in serum or plasma. Clin Chem 1993;38:578-82.
Trinder P. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann Clin Biochem 1969;6:24.
Sudhakar NS, Pattabiraman TN. A new colourimetric method for the estimation of glycosylated hemoglobin. Clin Chin Acta 1981;109:267-74.
Varley H. Practical clinical biochemistry, Arnold Heinemann Publication Pvt. Ltd. 1976; p. 452.
Owen JA, Iggo JB, Scangrett FJ, Steward, IP. Determination of creatinine in plasma, serum, a critical examination. J Biochem 1954;58:426-37.
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin’s phenol reagent. J Biol Chem 1951;193:265-75.
Reitman S, Frankel S. A colourimetric method for the determination of serum glutamic oxalacetic & glutamic pyruvic transaminases. Am J Clin Pathol. 1957;28(1):56-63.
King EJ, Armstrong AR. Determination of serum and bile phosphatase activity. Can Med Assoc J 1934;31:56-63.
Parekh AC, Jung. Cholesterol determination with ferric acetate, uranium acetate and sulphuric acid, ferrous sulphate reagent. Anal Chem 1970;112:1423-27.
Rice EW. Triglycerides in serum In: Standard Methods in Clinical Chemistry, (Ed) Roderick MP, Academic Press: New York; 1970. p. 215-22.
Warnick GR, Nguyan T, Albers AA. Comparison of improved precipitation methods for quantification of high-density lipoprotein cholesterol. Clin Chem 1985;31:217-22.
Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18(6):499-502.
Murat JC, Serfaty A: Simple enzymatic determination of Polysaccharide (Glycogen) content of animal tissue. Clin Chem 1974;20:1576-77.
Sinha AK. Colourimetric Assay of Catalase. Analytical Biochem 1972;47:389-394.
Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG, Science 1973;179:588-590.
Kakkar P, Das B, Viswanathan PN. Indian J Biochem Biophys 1984;2;130-32.
Uchiyama M, Mihara M. Determination of malonaldehyde precursor in the tissues of thiobarbituric acid test. Anal Biochem 1978;86:271-278.
Ellaman GL. Tissue sulfhydryl group. Arch Biochem Biophys 1995;82:70-77.
Wild S, Roglic G, Green A, et al. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 2004; 27: 1047-53.
Wild S, Roglic G, Green A, Sicree R, King H. Global Prevalence of Diabetes, Estimates for the year 2000 and projections for 2030. Diabetes Care 2004;27:1047-53.
Kumar A, Goel MK, Jain RB, Khanna P, Chaudhary V. India towards diabetes control: Key issues. Australasian Med J 2013;6(10):524-31.
Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res 2001;50:536-46.
Veeramani C, Pushpavalli G, Pugalendi KV. Antihyperglycaemic effect of Cardiospermum halicacabum Linn. leaf extract on streptozotocin induced diabetic rats. J Appl Biomed 2008;6:19-26.
Sezik E, Aslan M, Yesilada E, Ito S. Hypoglycaemic activity of Gentiana olivieri and isolation of the active constituent through bioassay-directed fractionation techniques. Life Sci 2005;76:1223-38.
Lal SS, Sukla Y, Singh A, Andriyas EA, Lall AM. Hyperuricemia, high serum urea and hypoproteinemia are the risk factor for diabetes. Asian J Med Sci 2009;1:33-4.
Deguchi Y, Miyazaki K. Anti-hyperglycemic and antihyperlipidemic effects of guava leaf extract. Nutr Metab 2010;7:9.
Alagammal M, Nishanthini A, Mohan VR. Antihyperglycemic and antihyperlipidaemic effect of Polygala rosmarinifolia Wright and Arn on alloxan induced diabetic rats. J Appl Pharm Sci 2012;2:143-8.
Lanjhiyana S, Garabadu D, Ahirwar D, Bigoniya P, Rana AC, Patra KC, et al. Antidiabetic activity of methanolic extract of stem bark of Elaeodendron glaucum Pers. in alloxanized rat model. Adv Appl Sci Res 2011;2:47-62.
Kala SMJ, Tresina PS, Mohan VR. The antioxidant, antihyperlipidaemic and antidiabetic activity of Eugenia singampattiana Bedd. leaves in alloxan induced diabetic rats. Int J Pharm Pharm Sci 2012;4:412-6.
Alagammal M, Paulpriya K, Mohan VR. Evaluation of antiinflammatory activity of ethanol extract of Polygala javana DC. whole plant. Int Res J Pharm 2012;3:212-3.
Palanivel R, Thangavel M, Selvendran K, Sakthisekaran D. Insulinomimetric effect of ammonium paratungstate on protein metabolism in streptozotocin-induced diabetic rats. Biomedical 2001;21:23-30.
Stanley P, Prince M, Menon VP. Hypoglycemic and other related actions of Tinospora cordifolia roots in alloxan induced diabetic rats. J Ethnopharmacol 1999;70:9-15.
Ghosh S, Suryawanshi SA. Effect of Vinca rosea extract in the treatment of alloxan diabetes in male albino rats. Indian J Exp Biol 2001;39:748-59.
Mooradian AD. Dyslipidemia in type 2 diabetes mellitus. Nat Clin Pract Endocrinol Metab 2009;5:150-9.
Rajalingam R, Srinivasan N, Govindarajulu P. Effect of alloxan induced diabetes on lipid profiles in renal cortex and medulla of mature albino rats. Indian J Exp Biol 1993;31:577-9.
Pathak RM, Ansai S, Mahmood A. Changes in chemical composition of intertional brush border membrane in alloxan induced chronic diabetes. Indian J Exp Biol 1981;19:503-5.
Maruthupandian A, Mohan VR, Sampathraj. The antidiabetic, anti-hyperlipidaemic and antioxidant activity of Wattakaka volubilis (L.F) Stapf. leaves in alloxan induced diabetic rats. Int J Pharm Sci Res 2010;1:83-90.
Mironova MA, Klein RL, Virella GL, Lopes-Virella MF. Antimodified LDL antibodies, LDL-containing immune complexes and susceptibility of LDL to in-vitro oxidation in patients with type-2 diabetes. Diabetes 2000;49:1033-49.
Scott M, Grundy. Diabetis cardiovas. Dis Circ 1999;100:1134-46.
Flowers on brain lipid peroxidation in rats treated with streptozotocin. Mol Cell Biochem 2003;243:23-8.
Ananthan R, Latha M, Ramkumar KM, Pari L, Basker L, Narmatha BV. The Antidiabetic effect of Gymnema montanum leaves Effect on lipid peroxidation induced oxidative stress in experimental diabetes. Nutrition 2004;6:379-86.
Pari L, Latha M. Effect of Cassia auriculata flowers on blood sugar levels, serum and tissue lipids in streptozotocin diabetic rats. Singapore Med J 2002;43:617-21.
Prince PSM, Menon VP. Effect of Syzigium cumini in plasma antioxidants on alloxan-induced diabetes in rats. J Clin Biochem Nutr 1998;25:81-6.
Prince PSM, Kamalakkannan N, Menon VP. Antidiabetic and antihyperlipidaemic effect of alcoholic Syzigium cumini seeds in alloxan induced diabetic albino rats. J Ethnopharmacol 2004;91:209-13.
Prince PSM, Menon VP, Gunasekaran G. Hypolipidaemic action of Tinospora cordifolia root extract in alloxan diabetic rats. J Ethnophamacol 1999;14:4-16.
Latha M, Pari L. Modulatory effect of Scoparia dulcis in oxidative stress-induced lipid peroxidation in streptozotocin diabetic rats. J Med Food 2003;6:379-86.
Loci AS, Shaabha M, Khazraji AL, Hussain A, Twaija A. Hypoglycemic effect of a valuable extract of Artemisia herba-alba II. Effect of a valuable extract on some blood parameters in diabetic animals. J Ethnopharmacol 1994;43:167-71.
Nishanthini A, Mohan VR. Antioxidant activity of Polygala rosmarinifolia Wight and Arn. The whole plant in alloxan induced diabetic rats. Int Res J Pharm 2012;3:223-5.
Robinson BH. The role of manganese superoxide dismutase in health and disease. J Inherited Metab Dis 1998;21:598-603.
Brioukhanov AL, Netrusov AI. Catalase and superoxide dismutase: distribution, properties, and physiological role in cells of strict anaerobes. Biochem 2004;69:949-62.
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