IN VITRO ANTIBACTERIAL ACTIVITIES OF MARINE SPONGE-ASSOCIATED BACTERIA AGAINST PATHOGENIC VIBRIO SPP. CAUSES VIBRIOSIS IN SHRIMPS
DOI:
https://doi.org/10.22159/ijpps.2019v11i11.34814Keywords:
Anti-Vibrio, Bioactive compounds, GC-MS, Sponge-associated bacteria, 16S-rRNAAbstract
Objective: This study was aimed to isolate and screen marine sponge-associated bacteria producing anti-Vibrio compounds and to identify their compounds from the bacterial extract.
Methods: Sponge-associated bacteria were isolated by spread plate method. Their anti-Vibrio activity against Vibrio parahaemolyticus, V. harveyi, and V. vulnificus was determined by dual culture test. Three potential isolates were identified based on 16S-rRNA gene analysis. All isolates producing anti-Vibrio compounds was tested for their haemolytic characters in blood agar medium. Anti-Vibrio activity of the most potential isolate was also tested by using its supernatant, extract, and concentrated culture. Chemical composition of crude extract derived from that isolate was identified by GC-MS analysis.
Results: 68 bacterial isolates have been isolated from the marine sponge, Spongia sp., Svenzea sp., Ircinia sp., and Igernella sp. Of 68 isolates, 15 (22%) isolates had anti-Vibrio activities in various spectra against three Vibrio species, including V. harveyi, V. parahaemolyticus, and V. vulnificus. All isolates producing anti-Vibrio compounds were non-haemolytic. Bacterial isolates coded as D6.6, D6.19, and P4.17 have broad spectra. They could inhibit at least two Vibrio species as indicated by the clear zone formed around bacterial colonies. Based on 16S-rRNA, these isolates were closely related (similarity ≥ 99%) to Brevibacterium casei strain M Sw oHS, Bacillus altitudinis strain FJAT 47750, and Bacillus altitudinis strain PgBe190, respectively. D6.6 isolate was the most potential isolate, which could inhibit three Vibrio species. Consistently, its anti-Vibrio activity also confirmed by their supernatant, concentrated culture, and crude extract of that isolate. The crude extract derived from this isolate contained 10 major compounds that are biologically active.
Conclusion: This study suggests that 15 bacteria strains isolated from marine sponges were potentially could inhibit Vibrio’s growth in vitro. These isolate could be further explored as anti-Vibrio agent.
Downloads
References
Chandrakala N, Priya S. Vibriosis in shrimp aquaculture a review. Int J Scientific Res Sci Eng Tech 2017;2:27-33.
Lee LH, Mutalib NSA, Law JWF, Wong SH, Letchumanan V. Discovery on antibiotic resistance patterns of Vibrio parahaemolyticus in selangor reveals carbapenemase-producing Vibrio parahaemolyticus in marine and freshwater fish. Front Microbiol 2018;9:1-13.
Marin Lit; 2018. Available from: http://pubs.rsc.org/marinlit. [Last accessed on 10 Aug 2019].
Debbab A, Aly AH, Lin WH, Proksch P. Bioactive compound from marine bacteria and fungi. Microb Biotechnol 2010;3:544-63.
Hentschel U, Piel J, Degnan SM, Taylor MW. Genomic insights into the marine sponge microbiome. Nat Rev Microbiol 2012;10:641-5.
Thirumalairaj J, Shanmugasundaram T, Sivasankari K, Natarajaseenivasan K, Balagurunathan R. Isolation, screening and characterization of potent marine Streptomyces sp. PM105 against antibiotic-resistant pathogens. As J Pharm Clin Res 2015;8:439-43.
Murniasih T, Putra MY, Hadi TA. The antibacterial evaluation of Haliclona associated bacteria and the relating compounds derived from the host. Asian J Pharm Clin Res 2018;11:412-5.
Peela S, Porana S. Isolation and screening of novel streptomyces from sediment of the bay of bengal near srikakulam coast. Int J Curr Pharm Res 2017;9:40-4.
Prastya ME, Astuti RI, Batubara I, Wahyudi AT. Antioxidant, antiglycation and in vivo antiaging effects of metabolite extracts from marine sponge-associated bacteria. Ind J Pharm Sci 2019;81:344-53.
Utami AWA, Wahyudi AT, Batubara I. Toxicity, the anticancer and antioxidant activity of extract from marine bacteria associated with sponge Japsis sp. Int J Pharm Bio Sci 2014;5:917-23.
Safari WF, Chasanah E, Wahyudi AT. Antibacterial and anticancer activities of marine bacterial extracts and detection of genes for bioactive compound synthesis. Int J Pharm Pharm Sci 2016;8:55-9.
Priyanto JA, Astuti RI, Nomura J, Wahyudi AT. Bioactive compounds from sponge-associated bacteria: anticancer activity and NRPS-PKS gene expression in different carbon sources. Am J Biochem Biotechnol 2017;13:148-56.
Mayer AMS, Rodriguez AD, Berlinck RGS, Fusetani N. Review marine pharmacology in 2007-8: marine compounds with antibacterial, anticoagulant, antifungal, anti-inflammatory, antimalarial, antiprotozoal, anti-tuberculosis, and antiviral activities; affecting the immune and nervous system, and other miscellaneous mechanism of action. Comp Biochem Physiol 2011;153:191-22.
Wahyudi AT, Priyanto JA, Maharsiwi W, Astuti RA. Screening and characterization of sponge-associated bacteria producing bioactive compounds anti-Vibrio sp. Am J Biochem Biotechnol 2018;14:221-9.
Radjasa O. Antibacterial activity of sponge-associated bacteria isolated from North Java Sea. J Coast Dev 2007;10:143-50.
Marchesi JR, Sato T, Weightman AJ, Martin TA, Fry JC, Iom SJ, et al. Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 1998;64:795-9.
Ahmed MM, Khan MA, Rainsford KD. Synthesis of thiophene and NO-curcuminoids for anti-inflammatory and anti-cancer activities. Molecules 2013;18:1483-501.
Ahsan T, Chen J, Zhao X, Irfan M, Wu Y. Extraction and identification of bioactive compounds (eicosane and dibutyl phthalate) produced by Streptomyces strain KX852460 for the biological control of Rhizoctonia solani AG-3 strain KX852461 to control target spot disease in tobacco leaf. AMB Express 2017;7:1-9.
Willett KL, Wassenberg D, Lienesch L, Reichert W, Giulio RTD. In vivo and in vitro inhibition of CYP1A-dependent activity in Fundulus heteroclitus by the polynuclear aromatic hydrocarbon fluoranthene. Toxicol Appl Pharmacol 2001; 177:264-71.
Wang DC, Sun SH, Shi LN, Qiu DR, Li X, Wei DS, et al. Chemical composition, the antibacterial and antioxidant activity of the essential oils of Metaplexis japonica and their antibacterial components. Int J Food Sci Tech 2014;50:449-57.
Uddin SJ, Grice D, Tiralongo E. Evaluation of cytotoxic activity of patriscabratine, tetracosane and various flavonoids isolated from the Bangladeshi medicinal plant Acrostichum aureus. Pharm Bio 2012;50:1276-80.
Gao X, Liu Y, Miao L, Li E, Hou T, Liu Z. Mechanism of anti-Vibrio activity of marine probiotic strain Bacillus pumilus H2, and characterization of the active substance. AMB Express 2017;7:1-10.
Abubakar H, Wahyudi AT, Yuhana M. Skrining bakteri yang berasosiasi dengan spons Japsis sp. sebagai penghasil senyawa antimikroba. Il Kelautan 2011;16:35–40.
Leyton Y, Borquez J, Darias J, Cueto M, Diaz Marrero AR, Riquelme C. Diketopiperazines produces by an Bacillus species inhibits Vibrio parahaemolyticus. J Aquat Res Dev 2012;3:1-5.
Boucabeille C, Mengin Lecreulx D, Henkes G, Simonet J, Heijenoort JV. Antibacterial and hemolytic activities of linenscin OC2, a hydrophobic substance produced by Brevibacterium linens OC2. FEMS Microbiol Lett 1997;153: 295-301.
Kiran GS, Lipton AN, Priyadharsini S, Anitha K, Suarez LEC, Arasu MV, et al. Antiadhesive activity of poly-hydroxy butyrate biopolymer from a marine Brevibacterium casei MSI04 against shrimp pathogenic Vibrios. Microbiol Cell Fact 2014;13:1-12.
Published
How to Cite
Issue
Section
