CYTOTOXICITY OF CRUDE EXTRACT FROM SPONGE-ASSOCIATED BACTERIA AGAINST MOLT4 LEUKEMIC CELL LINES THROUGH APOPTOSIS
Keywords:
Sponge-associated bacteria, MOLT4, Anticancer, Cell cycleAbstract
Objective: The objectives of this study were to isolate bioactive compounds from sponge-associated bacteria and analyze their cytotoxicity against MOLT4 leukemic cell lines through apoptosis.
Methods: Crude extract of sponge-associated bacteria encoded SAB E 35, SAB E 38 and SAB E 40 was obtained by extraction using ethyl acetate. The cytotoxicity assay was determined by Cell Counting Kit 8. Apoptosis assay was determined by Annexin V Apoptosis Detection Kit. Further, cell cycle was determined by DNA-specific dye Propidium Iodide(PI).
Results: The ethyl acetate extract showed cytotoxicity effect on MOLT4 cell line. Based on cytotoxicity rate value, SAB E 35 has the strongest capability of inhibiting MOLT4. The estimated ethyl acetate extract concentration required to inhibit the growth of MOLT4 cells by 50% (IC50) was 263.93 µg/ml. SAB E 35 showed the strongest apoptosis effect on MOLT4 cell line based on its ability to decrease viable cells, that remained22.70 % and 37.07% cells on their late apoptosis phase. Cell cycle on treated cells resulted decrease in the number of cells in S phase, G0/G1 and G2/M, in addition to the accumulation of cells in sub-G1 fraction.
Conclusion: The results of this study demonstrated that sponge-associated bacterial extracts have cytotoxicity effect on MOLT4 cell lines. Other results indicated that apoptosis occurred in MOLT4 treated by the extract, confirmed by the result of cell cycle analysis. The results suggested that the apoptotic effects of sponge-associated bacteria on MOLT4 can provide contribution to their claimed-anticancer activity.
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References
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