EVALUATION OF GASTROPROTECTIVE EFFECTS OF METHANOL EXTRACT OF TEPHROSIA VILLOSA AGAINST EXPERIMENTALLY INDUCED ULCERS IN WISTAR RATS

PINKEY RAWAL; RAMESH C.; SOMA PRAMANIK

doi:10.22159/ijpps.2021v13i11.41277

Authors

PINKEY RAWAL Department of Pharmaceutical Chemistry, East West College of Pharmacy, Bangalore 560091
RAMESH C. Department of Pharmacology, East West College of Pharmacy, Bangalore 560091
SOMA PRAMANIK Department of Pharmaceutical Chemistry, East West College of Pharmacy, Bangalore 560091

DOI:

https://doi.org/10.22159/ijpps.2021v13i11.41277

Keywords:

Tephrosia villosa, Anti-ulcer activity, Ethanol, Aspirin Ulcer index, pH, Total acidity, Percentage inhibition and percentage of protected area

Abstract

Objective: The present research work was designed to investigate the gastroprotective potentials of methanol extract of Tephrosia villosa.

Methods: The aerial parts of Tephrosia villosa were dried under shade, powdered and deffated with petroleum ether and then marc leftover was subjected to methanol extraction using soxhlet apparatus using soxh-let apparatus. Antiulcer activity of methanol extract was determined against stress-induced and aspirin-induced ulcers in experimental animal models. The total number of ulcers formed, ulcer index, percentage inhibition, ulcerated area, protected area, pH and Total acidity were parameters in the study.

Results: Methanol extract of Tephrosia villosa at medium (200 mg/kg) and high (200 mg/kg) could significantly (P<0.01) reduced the total number of ulcers formed, ulcer index, ulcerated area and total acidity in therapeutic groups compared to vehicle control and thereby significantly (P<0.01) increased percentage inhibition of ulcers and protected area which was evident by the significant rise in pH of gastric content. The effect of extracts was dose-dependent and results were comparable to that of standard drug omeprazole.

Conclusion: The results obtained from the present work suggest that the methanol extract of Tephrosia villosa possess significant anti-ulcer potentials against experimentally induced ulcers in albino rats.

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References

Hussain L, Akash MS, Naseem S, Rehman K, Ahmed KZ. Anti-ulcerogenic effects of salmalia malabarica in the gastric ulceration-pilot study. Adv Clin Exp Med. 2015;24(4):595-605. doi: 10.17219/acem/28115, PMID 26469103.

Scanlon VC, Sanders T. Essential of anatomy and physiology. 5th ed. Philadelphia: F. A. Davis company; 2007. p. 396.

Toma W, Hiruma Lima CA, Guerrero RO, Brito AR. Preliminary studies of Mammea americana L. (Guttiferae) bark/latex extract point to an effective antiulcer effect on gastric ulcer models in mice. Phytomedicine. 2005;12(5):345-50. doi: 10.1016/j.phymed.2003.06.009, PMID 15957368.

Al-Yahya MA, Rafatullah S, Mossa JS, Ageel AM, Al-Said MS, Tariq M. Gastric antisecretory, antiulcer and cytoprotective properties of ethanolic extract of Alpinia galanga willd in rats. Phytother Res. 1990;4(3):112-4. doi: 10.1002/ ptr.2650040308.

Santin JR, Lemos M, Klein Junior LCK, Niero R, de Andrade SF. Antiulcer effects of Achyrocline satureoides (Lam.) DC (Asteraceae) (Marcela), a folk medicine plant, in different experimental models. J Ethnopharmacol. 2010;130(2):334-9. doi: 10.1016/j.jep.2010.05.014, PMID 20546870.

Saldanha CJ, Singh BG. Leguminosae. In: Saldanha CJ, editor Flora of Karnataka. Vol. I. Oxford and IBH; 1984. p. 495-9.

Sarin JPS, Singh S, Garg HS, Khanna NM, Dhar MMA. Flavonol glycoside with anticancer activity from Tephrosia candida. Phytochemistry. 1976;15(1):232-4. doi: 10.1016/S0031-9422(00)89101-3.

Yuh-Lin C. New piscicidal flavonoids from Tephrosia obovata merr. Asian J Pharm. 1978;3(4):18.

Bentley MD, Hassanali A, Lwande W, Njoroge PEW, Yatagai M. Insect anti feed ants from Tephrosia elata Deflers. Insect Sci Appl. 1987;8(1):85-8.

Gokhale AB, Saraf MN. Tephrosia purpurea, a review of contemporary literature and medicinal properties. Indian Drugs. 2000;37:12.

Kapoor SL, Kapoor LD. Medicinal plant wealth of the Karimnagar District of Andhra Pradesh. Bull Ethnobotanical Res. 1980;1:120-44.

Jain SR, Sharma SN. Hypoglycaemic drugs of Indian indigenous origin. Planta Med. 1967;15(4):439-42. doi: 10.1055/s-0028-1100005, PMID 5603487.

Ramadhani SON, Zakaria HM, Abdul WK, Ester MI, Matobola JM, Paul E, Mainen JM. Larvicidal, antimicrobial and brine shrimp activities of extracts from Cissampelos mucronata and Tephrosia villosa from coast region, Tanzania. BMC Comp Alt Med. 2011;11:33.

Sayed DA, Fahmy SR, Soliman AM, Hussein NS. Antiulcerogenic efficacy of ethanolic extract of Vitis vinifera leaves in rats. Int J Pharm Pharm Sci. 2016;8(9):163-72. doi: 10.22159/ijpps.2016v8i9.12846.

Kokate CK. Practical pharmacognosy. New Delhi, Vallabh Prakashan. 1994;4:110-1.

Khandelwal KR. Practical pharmacognosy-techniques and experiments. Pune; NiraliPrakashan; 2000.

Trease GE, Evans MC. Text book of pharmacognosy London. Bailliare Tindall. 1983;12(193):336.

OECD. Acute oral toxicity-acute oral toxic class method. Guideline 423, adopted 23.03.1996. In: Eleventh addendum to the OECD guidelines for the testing of chemicals. Paris: Organization for Economic Co-operation and Development; 2000.

Vogel H. Drug discovery and evaluation pharmacological assays.2nd. 2002 ed. New York: Springer-Verlag Berlin Heidelberg; 2002. p. 870-5.

Aitken J, Fisher H. Reactive oxygen species generation and human spermatozoa: the balance of benefit and risk. Bioessays. 1994;16(4):259-67. doi: 10.1002/bies.950160409, PMID 8031303.

Rasika DB, Mahendra A, Sneha JA, Subodh CP. Antiulcer activity of the ethanol extract of leaves of Sesbania grandiflora (linn.). Int J Pharm Pharm Sci. 2010;2(4):206-8.

Vanita K, Deepali M. Evaluation of antipyretic and antiulcer activity of ethanolic extract of leaves of Alstonia scholaris l. in albino wistar rats. Asian J Pharm Clin Res. 2012;12(12):203-8.

Agrawal R, Garg HK, Garg U, S Mgh Sk. Antiulcer activity of Smithia conferta in various animal. J Sand Chem Soc. 2010;14:307-10.

Shreedhara CS, Aswatha Ram HN, Sachin B, Zanwar. Free radical scavenging activity of aqueous root extract of Argyreia nervosa. J Nat Rem. 2009;9:216-23.

Van JR. Inhibition of prostaglandin synthesis as a mechanism of action of aspirin-like drugs. Nature. 1971;235:231-5.

Szabo S, Trier JS, Brown A, Schnoor J. Early vascular injury and increased vascular permeability in gastric mucosal injury caused by ethanol in the rat. Gastroenterology. 1985;88(1 Pt 2):228-36. doi: 10.1016/s0016-5085(85)80176-1, PMID 3871087.

Konturek SJ, Piastucki I, Brzozowski T, Radecki T, Dembińska Kiec A, Zmuda A, Gryglewski R. Role of prostaglandins in the formation of aspirin-induced gastric ulcers. Gastroenterology. 1981;80(1):4-9. doi: 10.1016/0016-5085(81)90182-7. PMID 7450410.

Schubert ML. Pharmacotherapy for acid/peptic disorders. Yale J Biol Med. 1996;69(2):197-201. PMID 9112751.

Bhattacharya SK, Bhattacharya A, Kumar A, Ghosal S. Antioxidant activity of Bacopa monniera in rat frontal cortex, striatum and hippocampus. Phytother Res. 2000;14(3):174-9. doi: 10.1002/(sici)1099-1573(200005)14:3<174::aid-ptr624>3.0.co;2-o, PMID 10815010.

Rao ChV, Ojha SK, Radhakrishnan K, Govindarajan R, Rastogi S, Mehrotra S, Pushpangadan P. Antiulcer activity of Utleria salicifolia rhizome extract. J Ethnopharmacol. 2004;91(2-3):243-9. doi: 10.1016/j.jep.2003.12.020, PMID 15120446.

Cochran T, Stefanko J, Moore C, Saik R. Dimethyl sulfoxide protection against gastric stress ulceration. Curr Surg. 1983;40(6):435-7. PMID 6653164.

Kunchandy E, Rao MNA. Oxygen radical scavenging activity of curcumin. Int J Pharm. 1990;58(3):237-40. doi: 10.1016/0378-5173(90)90201-E.

Malfertheiner P, Chan FK, McColl KE. Peptic ulcer disease. Lancet. 2009;374(9699):1449-61. doi: 10.1016/S0140-6736(09)60938-7, PMID 19683340.

Govindarajan R, Rastogi S, Vijayakumar M, Shirwaikar A, Rawat AK, Mehrotra S, Pushpangadan P. Studies on the antioxidant activities of Desmodium gangeticum. Biol Pharm Bull. 2003;26(10):1424-7. doi: 10.1248/bpb.26.1424, PMID 14519948.