STANDARDIZATION AND APPLICATION OF PCR TARGETING CHLORELLA SPECIES ISOLATED FROM ENVIRONMENTAL SAMPLES

PREMINA S.; NIREN ANDREW S.; SUNDARALINGAM R.; N. MURUGAN; SHARANYA THERESA V.

doi:10.22159/ijpps.2021v13i7.41701

Authors

PREMINA S. Department of Microbiology, Madras Christian College, Chennai, Tamil Nadu, India 600059
NIREN ANDREW S. Department of Microbiology, Madras Christian College, Chennai, Tamil Nadu, India 600059
SUNDARALINGAM R. Department of Microbiology, Madras Christian College, Chennai, Tamil Nadu, India 600059
N. MURUGAN Infectious Diseases-Molecular Genetics Division, Life Cell International Pvt Ltd., Tamil Nadu, India 613401
SHARANYA THERESA V. Departmentt of Biotechnology, Loyola College, Chennai, Tamil Nadu, India 600036

DOI:

https://doi.org/10.22159/ijpps.2021v13i7.41701

Keywords:

Chlorella species, Microalgae, PCR, Primer designing, Sanger sequencing, Genotyping, Environmental samples

Abstract

Objective: Identification of Chlorella species from the environment through 18s ribosomal RNA sequencing. This study was aimed to design primer targeting Chlorella and other closely related algal species targeting 18s ribosomal RNA, ITS1 region.

Methods: Sanger sequencing was carried out for the identification of algae up to the genus and species level using an in-house designed primer and optimized PCR conditions.

Results: Out of 2 algae samples identified phenotypically, one isolate identified as Chlorella vulgaris and other one identified as Chlorella sorokiniana based on the results of Basic Alignment Search Tool (BLAST).

Conclusion: To conclude, this study provided primers with PCR conditions to characterize algal samples through molecular identification with 100% accuracy than the phenotypic method.

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