IN VITRO STUDIES ON Α-GLUCOSIDASE INHIBITION, ANTIOXIDANT AND FREE RADICAL SCAVENGING PROPERTIES OF TECOMA STANS L.
Keywords:
Nil, T stans, MeTs, Antioxidant etcAbstract
Objective: Tecomo. stans (T. stans)  (L) are plants extensively used for the empirical treatment of diabetes mellitus. But the bark part of T. stans (L) has not been extensively studied for the anti-diabetic and antioxidant property. Hence the current study were designed to estimate the in vitro activity of α-glucosidase inhibition and antioxidant property from bark extracts of T stans using various solvents such as hexane, ethyl acetate and methanol.
Methods: In the current study crude extract of T stans L. bark part has been evaluated for In vitro α-glucosidase inhibition and antioxidant activity from hexane, ethyl acetate and methanol extract using standard methods.
Results: Out of three solvents studied, methanol extract of T. stans L. (MeTs) showed 50% α-glucosidase inhibition at the concentration of 645.20±2.79 μg/ml. The total phenolic content of MeTs was 206.81±1.11 mg of catechol equivalents/g extract. MeTs showed great scavenging activity on 2, 2-diphenyl-picrylhydrazyl (DPPH) (IC50 765.25±0.42 µg/ml), hydroxyl (IC50 245.12±1.41 μg/ml), nitric oxide (IC50 800.10±1.05µg/ml) and superoxide (IC50 595.15±0.59 µg/ml) radicals, as well as high reducing power. MeTs also showed a strong suppressive effect on lipid peroxidation (IC50 810.15±2.19 µg/ml).
Conclusion: The results obtained in this study clearly indicate that MeTs has a significant potential to use as a natural a-glucosidase inhibition, antioxidant agent.
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Edeoga HO, Gomina A. Nutritional values of some non-conventional leafy vegetable in Nigeria. J Economic Taxonomic Botany 2000;24:7-13.
Kumar RA, Sridevi K, Kumar NV, Nanduri S, Rajagopal S. Anticancer and immuno stimulatory compounds from Andrographispaniculata. J Ethnopharmacol 2004;92:291-5.
Kaveeshwar, Seema Abhijeet, Jon Cornwall. The current state of diabetes mellitus in India. Australasian Med J 2015;45–8.
Mukherjee PK, Kumar V, Houghton PJ. Screening of Indian medicinal plants for acetyl cholinesterase inhibitory activity. Phytother Res 2007;21:1142-5.
Nagavani V, Rao TR. Evaluation of antioxidant potential and qualitative analysis of major polyphenols by RP-HPLC in NymphaeanouchaliBurm flowers. Int J Pharm Pharm Sci 2010;2:98-104.
Cartea ME, Francisco M, Lema M, Soengas P, Velasco P. Resistance of cabbage (Brassica oleraceacapitata group) crops to Mamestrabrassicae. J Econ Entomol 2010;103:1866-74.
Mundhe KS, Kale AA, Gaikwad SA, Deshpande NR, Kashalkar RV. Evaluation of phenol, flavonoid contents and antioxidant activity of Polyalthialongifolia. J Chem Pharm Res 2011;3:764-9.
Hakiman M, Maziah M. Non enzymatic and enzymatic antioxidant activities in aqueous extract of different Ficusdeltoidea accessions. J Med Plants Res 2009;3:120-31.
Marzouka M, Gamal-Eldeenb A, Mohamedc M, El-Sayedc M. Anti-Proliferative and Antioxidant Constituents from T. stans. Zeitschriftfür Naturforschung C; 2006. p. 783-91.
Alanso-Castro JA, Zapata-Bustos R, Romo-Yanez J, Camarillo-Ledesma P, Gomez-Sanchez M, et al. The antidiabetic plant T. stans (L.) Juss. exKunth (Bignoniaceae) and TeucriumcubensJacq (Lamiaceae) induce the incorporation of glucose in insulin sensitive and insulin-resistant murine and human adipocytes. J Ethnopharmacol 2010;127:62-9.
Nimal Christhudas IVS, Praveen Kumar P, Sunil C, Vajravijayan S, Lakshmi Sundaram R, Jenifer Siril S, et al. In vitro studies on α-glucosidase inhibition, antioxidant and free radical scavenging activities of Hedyotisbiflora L. Food Chem 2013;138:1689-95.
Sunil C, Ignacimuthu S. In vitro and in vivo antioxidant activity of Symplocoscochinchinensis S. Moore leaves containing phenolic compounds. Food Chem Toxicol 2011;49:1604–9.
Dahlqvist A. Method for assay of intestinal disaccharides. Anal Biochem 1964;7:18–25.
Slinkard K, Singleton VL. Total phenol analyses: Automation and comparison with manual methods. Am J Enol Vitic 1977;8:4955.
Hanato T, Kagawa H, Yasuhara T, Okuda T. Two new flavonoids and other constituents in licorice root: Their relative astringency and radical scavenging effects. Chem Pharm Bull 1988;36:2090–7.
Elizabeth K, Rao MNA. Oxygen radical scavenging activity of curcumin. Int J Pharm 1990;58:237–40.
Garratt DC. The quantitative analysis of drugs. Vol. 3. Japan: Chapman and Hall Ltd; 1964. p. 456–8.
Liu F, Ooi VEC, Chang ST. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci 1997;60:763–71.
Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Japan J Nutr 1986;44:307–15.
Yen GC, Hsieh CL. Antioxidant activity of extracts from Du-zhong (Eucommiaurmoides) towards various peroxidation models in vitro. J Agric Food Chem 1998;46:3952–7.
Brand-Williams W, Cuvelier M, Berset C. Use of a free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft und Technol 1995;28:25–30.
Hochestein P, Atallah AS. The nature of oxidant and antioxidant systems in the inhibition of mutation and cancer. Mutat Res 1988;202:363–75.
Babu BH, Shylesh BS, Padikkala J. Antioxidant and hepato protective effect of Alanthusicici focus. Fitoterapia 2001;72:272–7.
Marcocci L, Packer L, Droy-Lefai MT, Sekaki A, Gardes-Albert M. Antioxidant action of Ginkgo biloba extracts EGb 761. Methods Enzymol 1994;234:462–75.
Aruoma OI. Free radicals, oxidative stress and antioxidants in human health and disease. J Am Oil Chem Soc 1998;75:199–212.
Janero DR. Malondialdehyde and thiobarbituric acid reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radicals Biol Med 1990;9:515–40.
Meir S, Kanner J, Akiri B, Hadas SP. Determination and involvement of aqueous reducing compounds in oxidative defence systems of various senescing leaves. J Agric Food Chem 1995;43:1813–7.
Nitin KU, Yogendra Kumar MS, Asheesh G. Antioxidant, cytoprotective and antibacterial effects of Sea buck-thorn (Hippophaerhamnoides L.) leaves. Food Chem Toxicol 2010;48:3443–8.