ANTI-PROSTATE AND COLON CANCER AND DIURETIC EFFECTS OF QURANI PLANTS' MIXTURE (A NEW PHARMACEUTICAL PRODUCT)
Keywords:
Cytotoxicity, Colon cancer, Prostate cancer, Edible and medicinal plants, Nil, Diuretic activity, Urine analyses, Kidney functionsAbstract
Objective: QURANI plants' mixture is a new pharmaceutical product composed of some edible and medicinal plants (15 plants) mentioned in the Holy Quran (in a certain percentage, according to that is mentioned in Patent no. 1429/2013, presented to the Academy of Scientific Research and Technology, Egypt in 11/9/2013). The main aim of this work is to determine the cytotoxic effect of this extract against both human prostate (PC3) and colon (HCT116) cancer cell lines and also to evaluate the diuretic effect of this extract.
Methods: In vitro evaluation of the ethanolic extract of QURANI plants' mixture regarding its cytotoxic effect against both human prostate (PC3) and colon (HCT116) cancer cell lines was carried out. In vivo studies of the diuretic effect of daily feeding rats with 2, 4 and 8 g/kg of QURANI plants' mixture was performed also.
Results: In vitro studies of the ethanolic extract of QURANI plants' mixture revealed the cytotoxic effect of this extract against both human prostate (PC3) and colon (HCT116) cancer cell lines. Inhibition caused by using 100 µl/ml (this volume is related to 25 µg d. w. of QURANI plants' mixture) of this extract reached to 36.300±0.085 and 76.300±0.045 against HCT116 and PC3 cell lines, respectively. IC50 of this extract against PC3 cell line equals 44.60 µl/ml (this volume is related to 11.15 µg d. w. of QURANI plants' mixture), but it can not be detected in case of HCT116 cell line using these investigated concentrations of this extract. In vivo studies of the diuretic effect of daily feeding rats with 2, 4 and 8 g/kg of QURANI plants' mixture showed that, the amount of urine increased by increasing the amount of this mixture when compared to the control group. Results of kidney functions and urine analyses revealed that, this mixture has not any bad side effects in this regard, moreover, rats feeding with QURANI plants' mixture have better kidney functions (urea and creatinene are lower in case of rats feeding with QURANI plants' mixture than those of control group). Rats fed with this mixture are also having normal urine physical and chemical characters compared to the control group.
Conclusion: Results of anticancer and diuretic effects of QURANI plants' mixture will lead us to more biological and chemical investigations of this new, cheap and safe pharmaceutical natural product.
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References
The holy Quran.
Eman A Alam. Patent no: 1429/2013 (A new Pharmaceutical Product from Plants Mentioned in the Holy Quran), presented. Acad Sci Res Technol Egypt 11/9/2013.
Ghosh AK, Banerjee S, Mullick HI, Banerjee J. ZIngiber officinale: a natural gold. Int J Pharm Bio Sci 2011;2(1):283-94.
Cavallito CJ, Bailey JH, Allicin BJ. The antibacterial principle of Allium sativum. III. Its precursor and essential oil†of garlic. J Am Chem Soc 1945;67:1032-3.
Ross ZM, Maslin DJ, Hill DJ. The effect of steam distilled garlic oil on lactic acid and other enteric bacteria. 4th symposium on european microbiological societies. FEMS Microbiol Rev 2000;12:137.
Johnson OO, Ayoola1 GA, Adenipekun T. Antimicrobial activity and the chemical composition of the volatile oil blend from allium sativum (garlic clove) and citrus reticulate (tangerine fruit). Int J Pharm Sci Drug Res 2013;5(4):187-93.
Yukihiro K, Makoto I, Jiro Y, Naoki K, Naoto U, Isao S, et al. Pharmacokinetic study of allixin, a phytoalexin produced by garlic. Chem Pharm Bull 2002;50:352-63.
El-Hawary S, El-Fouly k, El Gohary HM, Meselhy KM, Slem A, Talaat Z. Phytochemical and biological investigation of vitis vinifera l. (flame cultivar), family vitaceae cultivated in egypt. Nat Sci 2012;10(10);48-59.
Al-Shahib W, Marshall RJ. The fruit of the date palm: Its possible use as the best food for the future. Int J Food Sci Nutr 2003;54:247-59.
Al-Farsi M, Alasalvar C, Morris A, Baron M, Shahidi F. Comparison of antioxidant activity, anthocyanins, carotenoids and phenolics of three native fresh and sun-dried date (Phoenix dactylifera L.) varieties grown in Oman. J Agric Food Chem 2005;53:7592-9.
Al-Showiman SS, Al Tamr, Ghetha wa Saha. Date, Food and Health. Dar Al-Khareji Press, Saudi Arabia; 1998.
Mansouri A, Embarek G, Kokkalou E, Kefalas P. Phenolic profile and antioxidant activity of the Algerian ripe date palm fruit (Phoenix dactylifera). Food Chem 2005;89:411-20.
Mohamed DA, Al-Okabi S. In vivo evaluation of antioxidant and anti-inflammatory activity of different extracts of date fruits in adjuvant arthritis. Polish J Food Nutr Sci 2004;13:397-402.
Guo C, Yang J, Wei J, Li Y, Xu J, Jiang Y. Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Nutr Res 2003;23:1719-26.
Kaur C, Kapoor HC. Antioxidants in fruits and vegetables: The millennium's health. International Journal of Food Sciences and Technology 2001;36:703-25.
Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol 2001;54:176-86.
Al-Humaid AI, Mousa HM, El-Mergawi RA, Abdel-Salam AM. Chemical composition and antioxidant activity of dates and dates-camel-milk mixtures as a protective meal against lipid peroxidation in rats. Am J Food Technol 2010;5:22-30.
Munisekhar Medasani. Natural extract from whole banana fruit (musa spp.). US patent. Publication Number; 2012.
Fioravanço JC. Mercado mundial da banana: produção, comércio eparticipação brasileira. Informações Econômicas 2003;33(10):15.
Jordan MJ, Tandon K, Shaw PE, Goodner KL. Aromatic profile of aqueous banana essence and banana fruit by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-olfactometry (GC-O). J Agric Food Chem 2001;49:4813-7.
Skehan P, Storeng R. New colorimetric cytotoxicity assay for anticancer drug screening. J Natl Cancer Inst 1990;82:1107-12.