EVALUATION OF IN VITROANTICANCER ACTIVITY AND GC-MS ANALYSIS FROM LEAF SOPHORA INTERRUPTA BEDD

Authors

  • Pardhasaradhi Mathi K L E F University
  • Kumar Nikhil Indian Institute of Technology Roorkee
  • Snehasish Das Indian Institute of Technology Roorkee
  • Partha Roy Indian Institute of Technology Roorkee
  • Venkata Raman Bokka Madanapalle Institute of Technology and Science (MITS)
  • Mahendran Botlagunta Biomedical research Laboratory, Department of Biotechnology, K L E F University, Green fields, Vaddeswaram 522502, Guntur District, Andhra Pradesh, India.

Keywords:

Sophora interrupta Bedd, Leaves, Methanol, Antioxidant, GC-MS

Abstract

Objective: Sophora interrupta Bedd (Fabaceae) is one of the well-known medicinal herb used in folk medicine for the treatment of cancer and inflammatory associated diseases. In this paper we aimed to evaluate the antioxidant and anti-cancer, properties of aqueous, methanol and n-hexane leaf extracts.

Methods: The leaf extracts were analyzed for antioxidant activity using DPPH free radical scavenging assay and anticancer activity by measuring the cell viability using MCF-7 and PC-3 cancer cell lines. GC-MS analysis was performed to identify anti-cancer compounds present in the active leaf extract.

Results: The polar solvent extracts showed good antioxidant at 500 μg/ml as related to non-polar solvents. Moreover, methanol extract exhibited highest percentage of cell death, in both MCF-7 (IC50 500 μg/ml) and PC-3 cells (IC50 1000 μg/ml), which is also evident from morphological observations, acridine orange and ethidium bromide dye exclusion assays.

Conclusion: Over all, it suggests that leaf methanol extract contain anticancer compounds, which are evident from our GC-MS analysis.

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Published

01-05-2015

How to Cite

Mathi, P., K. Nikhil, S. Das, P. Roy, V. R. Bokka, and M. Botlagunta. “EVALUATION OF IN VITROANTICANCER ACTIVITY AND GC-MS ANALYSIS FROM LEAF SOPHORA INTERRUPTA BEDD”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 5, May 2015, pp. 303-8, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/5093.

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