IN VITRO MULTIPLE SHOOT REGENERATION STUDIES IN CYPERUS ROTUNDUS L.
Keywords:
Cyperus rotundus L, MS (Murashige and Skoog) medium, Rhizome explants, In vitro regeneration, 2, 4-Dichloro phenoxy acetic acidAbstract
Objective: Cyperus rotundus L (Cyperaceae) is one of the highly valuable, potent, multipurpose medicinal herb used in traditional medicine for treatment of several human alignments. To meet the growing herbal needs of pharma industry, we made an attempt on in vitro regeneration of C. rotundus by using rhizome explants.
Methods: Rhizome explants were inoculated on both solid and liquid Murashige and Skoog [MS] media supplemented with various concentrations of Kinetin [Kn], 2,4-Dichloro phenoxy acetic acid [2,4-D], Naphthalene acetic acid [NAA], Indole-3-butyric acid [IBA] and activated charcoal for in vitro regeneration of multiple shoots and root induction.
Results: Efficient multiple shoots induction (88.9%) was observed on MS liquid media containing 3.0 mg/l 2,4-D+3.0 mg/l NAA+0.5 mg/l Kn after two weeks of explant inoculation with an average number of shoots 9.56±0.35 per explant. Further, we noticed effective root induction (82.3%) from these in vitro grown shoots on root induction media containing 1.0 mg/l IBA with 500 mg/l activated charcoal.
Conclusion: Overall, it suggests that this rapid and reliable protocol will be useful for mass multiplication of plantlets and maintenance of germ plasm throughout the year without seasonal constraints.
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