IN VITRO CYTOTOXICITY AND ANTIMICROBIAL EFFICACY OF THEVETIA PERUVIANA SEED KERNEL EXTRACTS
Keywords:
In vitro cytotoxicity, Tumor cell lines, Skin pathogens, IC50 valuesAbstract
Objective: Whole plant and various parts of Thevetia peruviana were reported to possess several pharmacological properties, due to the vast array of secondary metabolites. The present investigation was carried out to illustrate the cytotoxic and antimicrobial properties of seed kernel extracts on two tumor cell lines and various human skin-pathogenic microbes.
Methods: Cold methanol extracts and successive soxhlet fractions of seed samples were incubated with tumor cell lines in vitro for 3 h at 37 oC, and cell viability was assessed by trypan blue exclusion method. Antimicrobial efficacy was detected by well diffusion protocol against five bacterial strains and two fungal strains at varying concentrations ranging from 10-0.025 mg/6 mm wells, using different fractions from three color variants.
Results: Of the evaluated samples, cold extract of seed kernels has potent cytotoxicity, with IC50 values 0.458 and 0.522 mg/ml on both cell lines than soxhleted ethyl acetate (EA) fractions (0.851 and 0.712 mg/ml). Antimicrobial efficacy against Pseudomonas aeruginosa (18.67±1.15 mm), Nocardia asteroids (15.00±0.00) and Candida albicans (14.33±0.58) strains were effective for EA (10 mg/well) than chloroform (CH) and methanol (MT) fractions, but lower concentrations of CH fractions showed higher sensitivity against C. albicans.
Conclusion: Cytotoxic properties of seed kernels could be utilized against various human cancer cell lines to reduce tumor growth, and the bactericidal and fungicidal efficacy would be helpful in eradicating some common human skin infectious pathogenic microbes.
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