ANTIMUTAGENIC EFFECTS OF ETHANOL EXTRACT OF SYZYGIUM MYRTIFOLIUM WALP. IN CYCLOPHOSPHAMIDE-INDUCED MICE

Irene Sondang Lingga; Urip Harahap; Yuandani Yuandani

doi:10.22159/ajpcr.2018.v11i5.24350

Authors

Irene Sondang Lingga Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
Urip Harahap Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
Yuandani Yuandani Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i5.24350

Keywords:

Pucuk merah, Syzygium myrtifolium Walp, Antimutagenic

Abstract

Objective: This current study was carried to evaluate the antimutagenic effects of ethanol extract Syzygium myrtifolium Walp. in cyclophosphamide-induced mice.

Methods: The mice were divided into 6 groups with the total number of 30 mice (CMC Na 0,5%; cyclophosphamide 30 mg/kg bw; and extract 50, 100, 200, and 400 mg/kg bw) consisting of 5 rats for each group. All mice were induced by cyclophosphamide 30 mg/kg bw on day 1, then followed by administration of extract for 7 days. On day 8, the blood was collected to evaluate the hematocrit value, while the femoral bone marrow was collected to determine the number of micronucleus cells.

Results: The ethanol extracts of S. Myrtifolium leaves at doses of 50â€“400 mg/kg bw demonstrated antimutagenic effect by inhibiting the formation of micronucleus as compared to negative control (CMC Na 0.5%). Meanwhile, the hematocrit value increased at dose of 100, 200, and 400 mg/kg bw as compared to negative control.

Conclusion: This finding suggests that ethanol extract of S. myrtifolium has antimutagenic activity that may increase the normal cell number in cyclophosphamide-induced mice.

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