THERAPEUTIC IMPACT OF BERRIES (MORUS ALBA AND MORUS RUBRA) FRUIT EXTRACT IN THE REGRESSION OF HIGH-FAT DIET-INDUCED CARDIAC DYSFUNCTION IN RATS

Farouk K El-baz; Hanan F Aly; Howaida I Abd-alla; Dalia F Biomy

doi:10.22159/ajpcr.2018.v11i7.25859

Authors

Farouk K El-baz Department of Plant Biochemistry, National Research Centre, 33 El Bohouth St. (Former El Tahrir St.), Dokki, Giza, P.O.12622, Egypt. http://orcid.org/0000-0002-4750-3559
Hanan F Aly Department of Therapeutic Chemistry, National Research Centre, 33 El Bohouth St. (Former El Tahrir St.), Dokki, Giza, P.O.12622, Egypt.
Howaida I Abd-alla Department of Chemistry of Natural Compounds, National Research Centre, 33 El Bohouth St. (Former El Tahrir St.), Dokki, Giza, P.O.12622, Egypt.
Dalia F Biomy Department of Therapeutic Chemistry, National Research Centre, 33 El Bohouth St. (Former El Tahrir St.), Dokki, Giza, P.O.12622, Egypt.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i7.25859

Keywords:

Mulberry fruit, Cardiac dysfunction, Cardiac fibrosis, Obesity

Abstract

Objective: The aim of the present study is to investigate the effect of Morus alba (MA) and Morus rubra L. (MR) fruit extract on obesity-induced cardiac dysfunction and fibrosis in cardiac tissue.

Methods: Seventy male Wistar albino rats were randomly divided into five groups of ten rats each. MA and MR have been administered for 6 weeks in obese rats induced by high-fat diet (HFD). Adiponectin, glucagon, troponin, plasminogen activator inhibitor, cell adhesion molecules-1 (intracellular and vascular respectively), C-reactive protein, collagen type II and collagen alpha-1 (III) chain, and lipoxygenase activity were also estimated in serum of obese rats. Histopathological investigation of cardiac tissue was carried out.

Results: MA and MR treatments significantly normalized cardiac dysfunction biomarkers as well as cardiac fibrosis as examined by histopathological examination with higher percentage of improvement for MR extract.

Conclusion: Hence, it could be concluded that MA and MR extracts have useful effects on obesity-associated cardiac diseases through lipid metabolism regulation, cardiac functions and reversed cardiac fibrosis.

Downloads

Download data is not yet available.

Author Biography

Farouk K El-baz, Department of Plant Biochemistry, National Research Centre, 33 El Bohouth St. (Former El Tahrir St.), Dokki, Giza, P.O.12622, Egypt.

Plant Biochemistry

References

Lim HH, Yang SJ, Kim Y, Lee M, Lim Y. Combined treatment of mulberry leaf and fruit extract ameliorates obesity-related inflammation and oxidative stress in high fat diet-induced obese mice. J Med Food 2013;16:673-80.

Rout SP, Choudary KA, Kar DM, Das L, Jain A. Plants in traditional medicinal system-future source of new drugs. Int J Pharm Pharm Sci 2009;1:1-23.

El-Baz FK, Aly HF, Abd-Alla HI. Berries supplementation modulates body weight and metabolic deteriorations in obese rats. Asian J Pharm Clin Res 2018;11:322-8.

Rao SJ, Ramesh CK, Mahmood R, Prabhakar BT. Anthelmintic and antimicrobial activities in some species of mulberry. Int J Pharm Pharm Sci 2012;4:335-8.

Lee CY, Cheng HM, Sim SM. Mulberry leaves protect rat tissues from immobilization stress-induced inflammation. Biofactors 2007;3:25-33.

Ou TT, Hsu MJ, Chan KC, Huang CN, Ho HH, Wang CJ. Mulberry extract inhibits oleic acid-induced lipid accumulation via reduction of lipogenesis and promotion of hepatic lipid clearance. J Sci Food Agric 2011;91:2740-8.

Azman KF, Amom Z, Azlan A, Esa NM, Ali RM, Shah ZM, et al. Antiobesity effect of Tamarindus indica L. pulp aqueous extract in high-fat diet-induced obese rats. J Nat Med 2012;66:333-42.

Ann JY, Eo H, Lim Y. Mulberry leaves (Morus alba L.) ameliorate obesity-induced hepatic lipogenesis, fibrosis, and oxidative stress in high-fat diet-fed mice. Genes Nutr 2015;10:1-13.

Adaramoye O, Akinatyo O, Achen J, Michel A. Lipid lowering effects of methanolic extracts of Vernonia anygdalina leaves in rats fed on high cholesterol diet. Vasc Health Risk Manag 2008;4:235-41.

Shalaby HM, Tawfek NS, Abo-El Hussein BK, Abd El-Ghany MS. The assessment of some biochemical and immunological effects by amphetamine and orlistat on obesity in rats. Food Public Health 2015;4:185-92.

Hwang YJ, Lee EJ, Kim HR, Hwang KA. In vitro antioxidant and anticancer effects of solvent fractions from Prunella vulgaris var. Lilacina. BMC Complement Altern Med 2013;13:310.

Selek S, Aslan M, NazlÄ±gul Y. Serum PON1 activity and oxidative stress in non-alcoholic fatty liver disease. J Harran Univ Med Fac 2012;9:85-91.

Drury RA, Wallington EA. Carletonâ€™s Histology Technique. 4th ed. New York: Oxford University Press; 1980. p. 653-61.

Davidson EP, Coppey LJ, Calcutt NA, Oltman CL, Yorek MA. Diet-induced obesity in sprague-dawley rats causes microvascular and neural dysfunction. Diab Metab Res Rev 2010;26:306-18.

Davidson EP, Coppey LJ, Dake B, Yorek MA. Effect of treatment of Sprague dawley rats with AVE7688, enalapril, or candoxatril on diet-induced obesity. J Obesity 2011;2011:pii: 686952.

Marques C, Meireles M, Norberto S, Leite J, Freitas J, Pestana D, et al. High-fat diet-induced obesity rat model: A comparison between wistar and Sprague-dawley rat. Adipocyte 2016;5:11-21.

Bullen JW Jr., Bluher S, Kelesidis T, Mantzoros CS. Regulation of adiponectin and its receptors in response to development of diet-induced obesity in mice. Am J Physiol Endocrinol Metab 2007;292:1079-86.

Aly HF, Abd-Alla HI, Ali SA, Aba-Alez R, Abu-Krisha MT, Mohamed MM. Bioinformatics: inflammatory cytokines and attenuation of diabetes hypercholesterolemia-induced renal injury using morning glory and necklace pod extracts. Asian J Pharm Clin Res 2017;10:347-55.

Wang H, Storlien LH, Huang XF. Effects of dietary fat types on body fatness, leptin, and ARC leptin receptor, NPY, and AgRP mRNA expression. Am J Physiol Endocrinol Metab 2002;282:E1352-9.

De Martini T, Nowell M, James J, Williamson L, Lahni P, Shen H, et al. High fat diet-induced obesity increases myocardial injury and alters cardiac STAT3 signaling in mice after polymicrobialsepsis. Biochim Biophys Acta 2017;1863:2654-60.

Si LY, Ali SA, Latip J, Fauzi NM, Budin SB, Zainalabidin S. Roselle is cardio protective in diet-induced obesity rat model with myocardial infarction. Life Sci 2017;191:157-65.

Abdurrachim D, Ciapaite J, Wessels B, Nabben M, Luiken JJ, Nicolay K, et al. Cardiac diastolic dysfunction in high-fat diet fed mice is associated with lipotoxicity without impairment of cardiac energetics in vivo. Biochim Biophys Acta 2014;1842:1525-37.

Barnard SA, Pieters M, De Lange Z. The contribution of different adipose tissue depots to plasma plasminogen activator inhibitor-1 (PAI-1) levels. Blood Rev 2016;30:421-9.

Freeman LR. Cerebrovascular Changes: The Role of fat and Obesity. Omega-3 Fatty Acids in Brain And Neurological Health. New York: Academic Press; 2014. p. 221-9.

Kyrou I, Mattu HS, Chatha K, Randeva HS. Fat Hormones, Adipokines. Endocrinology of the Heart in Health and Disease. Amsterdam: Academic Press; 2017. p. 167-205.

Chen F, Chen D, Zhao X, Yang S, Li Z, Sanchis D, et al. Interleukin-6 deficiency facilitates myocardial dysfunction during high fat diet-induced obesity by promoting lipotoxicity and inflammation. Biochim Biophys Acta 2017;1863:3128-41.

Silva DC, Lima-Leopoldo AP, Leopoldo AS, Campos DH, Nascimento AF, Oliveira SA Jr., et al. Influence of term of exposure to high-fat diet-induced obesity on myocardial collagen Type I and III. Arq Bras Cardiol 2014;102:157-64.

Schram K, De Girolamo S, Madani S, Munoz D, Thong F, Sweeney G. Leptin regulates MMP-2, TIMP-1 and collagen synthesis via p38 MAPK in HL-1 murine cardiomyocytes. Cell Mol Biol Lett 2010;15:551-63.

Carroll JF, Tyagi SC. Extracellular matrix remodeling in the heart of the homocysteinemic obese rabbits. Am J Hypertens 2005;18:692-8.

Leopoldo AS, Sugizaki MM, Lima-Leopoldo AP, do Nascimento AF, Luvizotto Rde A, de Campos DH, et al. Cardiac remodeling in a rat model of diet-induced obesity. Can J Cardiol 2010;26:423-9.

Chakrabarti SK, Wen Y, Dobrian AD, Cole BK, Ma Q, Pei H, et al. Evidence for activation of inflammatory lipoxygenase pathways in visceral adipose tissue of obese zucker rats. Am J Physiol Endocrinol Metab 2011;300:175-87.

Aramwit P, Supasyndh O, Siritienthong T, Bang N. Mulberry leaf reduces oxidation and C-reactive protein level in patients with mild dyslipidemia. Biomed Res Int 2013;2013:787981.

Peng CH, Liu LK, Chuang CM, Chyau CC, Huang CN, Wang CJ. Mulberry water extracts possess an anti-obesity effect and ability to inhibit hepatic lipogenesis and promote lipolysis. J Agric Food Chem 2011;59:2663-71.

Doi K, Kojima T, Fujimoto Y. Mulberry leaf extract inhibits the oxidative modification of rabbit and human low density lipoprotein. Biol Pharm Bull 2000;23:1066-71.

Liu GS, Zhang ZS, Yang B, He W. Resveratrol attenuates oxidative damage and ameliorates cognitive impairment in the brain of senescence-accelerated mice. Life Sci 2012;91:872-7.

Park S, Choi Y, Um SJ, Yoon SK, Park T. Oleuropein attenuates hepatic steatosis induced by high-fat diet in mice. J Hepatol 2011;54:984-93.

Huang GC, Zhang JS, Tang QQ. Involvement of C/EBP-alpha gene in vitro activation of rat hepatic stellate cells. Biochem Biophys Res Commun 2004;324:1309-18.

Priya S. Medicinal values of mulberryâ€“an overview. J Pharm Res 2012;5:3588-96.

Choi EM, Hwang JK. Effects of Morus alba leaf extract on the production of nitric oxide, prostaglandin E2 and cytokines in RAW264.7 macrophages. Fitoterapia 2005;76:608-13.

Hosek J, Bartos M, Chudik S, DallAcua S, Innocenti G, Kartal M, et al. Natural compound cudraflavone B shows promising anti-inflammatory properties in vitro. J Nat Prod 2011;74:614-9.

Kim HJ, Lee HJ, Jeong SJ, Lee HJ, Kim SH, Park EJ. Cortex mori radicis extract exerts antiasthmatic effects via enhancement of CD4(+) CD25(+)Foxp3(+) regulatory T cells and inhibition of Th2 cytokines in a mouse asthma model. J Ethnopharmacol 2011;138:40-6.

Oh YC, Kang OH, Choi JG, Chae HS, Lee YS, Brice OO, et al. Anti-inflammatory effect of resveratrol by inhibition of IL-8 production in LPS-induced THP-1 cells. Am J Chin Med 2009;37:1203-14.