NEURORESTORATIVE MULBERRIES POTENTIAL OF ALZHEIMER'S DISEASE IN ANIMAL MODEL
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i10.27155Keywords:
Neurorestorative, Brain neurotransmitters, Nil, Morus, Polyphenols content, Histopathological assessmentAbstract
Objective: Alzheimer's disease (AD) is one of the most common neurodegenerative disorders. The present study aimed to identify the potential neurorestorative of Morus alba and Morus rubra fruits extract on aluminum chloride (AlCl3)-induced AD rats.
Methods: Each Morus species extract M. alba and M. rubra at a dose 300 mg/kg body weight (b.wt.) was orally administered, daily for 6 weeks post AlCl3 induction (100 mg/kg b.wt./day/6 weeks, orally). The polyphenols content was determined according to the colorimetric method of Folin–Ciocalteu using gallic acid as a standard. In addition, brain neurotransmitters, total protein content (TPC), and antioxidant biomarkers were determined. Histopathological assessment was performed on the brains of rats.
Results: Administration of AlCl3 neurotoxicity induced a significant increment of brain acetylcholinesterase, and nitric oxide, while significant decrease in four monoamines (norepinephrine, epinephrine, 5-hydroxytryptamine serotonin, and dopamin), along with brain tissue ATPase, and TPC were determined. AlCl3-induced changes in peroxidative products, such as protein carbonyl, catalase, and superoxide dismutase activity. AlCl3 induction resulted in significant decrease in antioxidant enzymes, serum level of total antioxidant capacity, and caused histological alteration for brain tissue. Treatment of AD-rats with M. alba and M. rubra (300 mg/kg b.wt./day) causes modulation of most biochemical markers brain function and confirmed by brain histopathological study.
Conclusion: Collectively, the present results indicated that M. rubra and M. alba exerts neuroprotection against AlCl3-induced brain pathology. To the best of our knowledge, none of the literature studies have demonstrated the effect of these Morus species against Al-neurotoxicity at biochemical and electron microscopic levels.
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Jiang Y, Gao H, Turdu G. Traditional Chinese medicinal herbs as potential AChE inhibitors for anti-Alzheimer’s disease: A review. Bioorg Chem 2017;75:50-61.
Yang Y, Wang JZ. Nature of tau-associated neurodegeneration and the molecular mechanisms. J Alzheimer’s Dis 2018;62:1305-17.
Kawahara M, Kato-Negishi M. Link between aluminum and the pathogenesis of Alzheimer’s disease: The integration of the aluminum and amyloid cascade hypotheses. Int J Alzheimers Dis 2011;2011:1-17.
Chang YT, Chang WN, Tsai NW, Huang CC, Kung CT, Su YJ, et al. The roles of biomarkers of oxidative stress and antioxidant in Alzheimer’s disease: A systematic review. Bio Med Res Int 2014;2014:1-14.
El-Baz FK, Hassan AZ, Abd-Alla HI, Aly HF, Mahmoud K. Phytochemical analysis, assessment of ant proliferative and free radical scavenging activity of Morus alba and Morus rubra fruits. Asian J Pharm Clin Res 2017;10:189-99.
Awad HM, Abd-Alla HI, Ibrahim MA, El-Sawy ER, Abd-Alla MM. Flavones from heavenly blue as modulators of alzheimer’s amyloid-beta peptide (Aβ) production. Med Chem Res 2018;27:768-76.
Awad HM, Abd-Alla HI, Mahmoud KH, El-Toumy SA. In vitro anti-nitrosative, antioxidant, and cytotoxicity activities of plant flavonoids: A comparative study. Med Chem Res 2014;23:3298-307.
Mohamed NZ, Abd-Alla HI, Aly HF, Mantawy M, Ibrahim N, Hassan SA. CCl4-induced hepatonephrotoxicity: Protective effect of nutraceuticals on inflammatory factors and ant oxidative status in rat. J Appl Pharm Sci 2016;4:87-100.
El-Baz FK, Aly HF, Abd-Alla HI. Berries supplementation modulates body weight and metabolic deteriorations in obese rats. Asian J Pharm Clin Res 2018;11:322-8.
Abd-Alla HI, Sweelam HM, Mohamed TA, Gabr MM, El-Safty MM, Hegazy MF. Efficacy of extracts and iridoid glucosides from Pentas lanceolata on humoral and cell-mediated immune response of viral vaccine. Med Chem Res 2017;26:2196-204.
Phukan P, Bawari M, Sengupta M. Promising neuroprotective plants from north-east India. Int J Pharm Pharm Sci 2015;7:28-39.
Zheng XK, Cao YG, Ke YY, Zhang YL, Li F, Gong JH, et al. Phenolic constituents from the root bark of Morus alba L. and their cardioprotective activity in vitro. Phytochemistry 2017;135:128-34.
Tan L, Yang HP, Pang W, Lu H, Hu YD, Li J, et al. Cyanidin-3-O-galactoside and blueberry extracts supplementation improves spatial memory and regulates hippocampal ERK expression in senescence-accelerated mice. Biomed Environ Sci 2014;27:186-96.
Chan EW, Lye PY, Wong SK. Phytochemistry, pharmacology, and clinical trials of Morus alba. Chin J Nat Med 2016;14:17-30.
Bourre JM. Roles of unsaturated fatty acids (especially omega-3 fatty acids) in the brain at various ages and during ageing. J Nutr Health Aging 2004;8:163-74.
Nag G, De B. Acetylcholinesterase inhibitory activity of Terminalia chebula, Terminalia bellerica and Emblica officinalis and some phenolic compounds. Int J Pharm Pharm Sci 2011;3:121-4.
Singleton, VL, Rossi, JA. Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. Am J Enol Viticult 1995;16:144-58.
Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol 2001;54:356-61.
Fernando HA, Chin HF, Ton SH, Kadir KA. Stress and its effects on glucose metabolism and 11β-HSD activities in rats fed on a combination of high-fat and high-sucrose diet with glycyrrhizic acid. Diabetes Res 2013;95:1903-13.
Zagrodzka J, Romaniuk A, Wieczorek M, Boguszewski P. Bicuculline administration into ventromedial hypothalamus: Effects on fear and regional brain monoamines and GABA concentrations in rats. Acta Neurobiol Exp 2000;60:333-43.
Matteucci E, Cocci F, Pellegrini L, Gregori G, Giampietro O. Measurement of ATPases in red cells: Setting up and validation of a highly reproducible method. Enzyme Protein 1994;48:105-19.
Bradford MM. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54.
Nishikimi M, Rae NA, Yagi K. The occurrence of superoxide anion in the action of reduced phenazine methosulphate and molecular oxygen. Biochem Biophys Res Commun 1972;46:849-53.
Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6.
Berkels R, Purol-Schnabel S, Roesen R. Measurement of nitric oxide by reconversion of nitrate/nitrite to NO. Methods Mol Biol 2004;279:1-8.
Padurariu M, Ciobica A, Hritcu L, Stoica B, Bild W, Stefanescu C. Changes of some oxidative stress markers in the serum of patients with mild cognitive impairment and Alzheimer’s disease. Neurosci Lett 2010;469:6-10.
Cristalli DO, Arnal N, Marra FA, De Alaniz MJ, Marra C. Peripheral markers in neurodegenerative patients and their first-degree relatives. J Neurol Sci 2012;314:48-56.
Liao YR, Kuo PC, Tsai WJ, Huang GJ, Lee KH, Wu TS. Bioactive chemical constituents from the root bark of Morus australis. Bioorg Med Chem Lett 2017;27:309-13.
Tavares L, Figueira I, McDougall GJ, Vieira HL, Stewart D, Alves PM, et al. Neuroprotective effects of digested polyphenols from wild blackberry species. Eur J Nutr 2013;52:225-36.
Guo H, Cao M, Zou S, Ye B, Dong Y. Cranberry extract standardized for proanthocyanidins alleviates β-Amyloid peptide toxicity by improving proteostasis through HSF-1 in Caenorhabditis elegans model of Alzheimer’s disease. J Gerontol A Biol Sci Med Sci 2016;71:1564-73.
Bagchi D, Bagchi M, Stohs SJ, Das DK, Ray SD, Kuszynski CA, et al. Mitochondrial- and endoplasmic reticulum-associated oxidative stress in Alzheimer’s disease: From pathogenesis to biomarkers. Int J Cell Biol 2012;2012:1-23.
Aly HF, Abd-Alla HI, Ali SA, Aba-Alez R, Abu-Krisha MT, Mohamed MM. Bioinformatics: Inflammatory cytokines and attenuation of diabetes hypercholesterolemia-induced renal injury using morning glory and necklace pod extracts. Asian J Pharm Clin Res 2017;10:347-55.
Sawikr Y, Yarla NS, Peluso I, Kamal MA, Aliev G, Bishayee A. Neuroinflammation in alzheimer’s disease: The preventive and therapeutic potential of polyphenolic nutraceuticals. Adv Protein Chem Struct Biol 2017;108:33-57.
Jung HA, Ali MY, Jung HJ, Jeong HO, Chung HY, Choi JS. Inhibitory activities of major anthraquinones and other constituents from Cassia obtusifolia against β-secretase and cholinesterases. J Ethnopharmacol 2016;191:152-60.
Vepsäläinen S, Koivisto H, Pekkarinen E, Mäkinen P, Dobson G, McDougall GJ, et al. Anthocyanin-enriched bilberry and blackcurrant extracts modulate amyloid precursor protein processing and alleviate behavioral abnormalities in the APP/PS1 mouse model of alzheimer’s disease. J Nutr Biochem 2013;24:360-70.
Wang J, Wang T, Xie P, Yin G, Li X. New phenanthrene derivatives with nitric oxide inhibitory and radical-scavenging activities from Pholidota imbricata Hook. Nat Prod Res 2014;28:251-6.
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