BIOINFORMATICS: INFLAMMATORY CYTOKINES AND ATTENUATION OF DIABETES HYPERCHOLESTEROLEMIA-INDUCED RENAL INJURY USING MORNING GLORY AND NECKLACE POD EXTRACTS

Hanan F Aly; Howaida I Abd-alla; Howaida I Abd-alla; Sanaa A Ali; Sanaa A Ali; Reda Aba Alez; Reda Aba Alez; Mohammed T Abu-krisha; Mohammed T Abu-krisha; Mohamed M Mamdouh; Mohamed M Mamdouh

doi:10.22159/ajpcr.2017.v10i11.20119

Authors

Hanan F Aly Department of Therapeutic Chemistry, National Research Centre, Dokki, Giza, Egypt http://orcid.org/0000-0001-9638-8481
Howaida I Abd-alla
Howaida I Abd-alla Department of Chemistry of Natural Compounds, National Research Centre, Dokki, Giza, Egypt
Sanaa A Ali
Sanaa A Ali Department of Therapeutic Chemistry, National Research Centre, Dokki, Giza, Egypt
Reda Aba Alez
Reda Aba Alez Department of Information Systems and Computer Engineering, Faculty of Engineering, Al-Azhar University, Cairo, Egypt
Mohammed T Abu-krisha
Mohammed T Abu-krisha Department of Information Systems and Computer Engineering, Faculty of Engineering, Al-Azhar University, Cairo, Egypt
Mohamed M Mamdouh
Mohamed M Mamdouh Department of Information Systems and Computer Engineering, Faculty of Engineering, Al-Azhar University, Cairo, Egypt

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i11.20119

Keywords:

Morning glory, Necklace pod, Inflammatory, Cell adhesion, Kidney function, Statistic, Image recognition

Abstract

Â

Â Objective: The present research in bioinformatics focuses on pharmacological effects of morning glory and necklace pod ethanolic extracts (MGE and NPE) on some biochemical parameters in high fat diet-induced hypercholesterolemia and streptozotocin-induced hyperglycemia in rats.

Methods: Compared to atorvastatin; an anti-hypercholesterolemic (HC) and glibenclamide; an antidiabetic drug. Endothelium activation markers of soluble vascular cell adhesion molecule (VCAM)-1 and intercellular adhesion molecule (ICAM)-1 were determined using enzyme-linked immunosorbent assay. Creatinine, urea, and inflammatory biomarkers; C-reactive protein (CRP) and pro-inflammatory cytokines including tumor necrosis factor alpha (TNF-Î±) and interleukin (IL)-10 levels were also measured in serum of different therapeutic groups.

Results: Significant decrease in ICAM-1 level with MGE and NPE supplemented to normal rats as compared to untreated control with percentages decrease 17.80 and 12.00% was observed. Insignificant change was detected in VCAM-1 level. Profound amelioration in CRP, total urea and creatinine levels by NPE treatment. Creatinine, urea, CRP, and TNF-Î± level were significantly increased in hyperglycemic (HG)-HC rats. However, IL-10 level showed a significant decrease. Meanwhile, histopathological investigation of the kidney and heart was carried out. Image recognition system for kidney and heart images was developed to diagnose their diseases. Tested extract attenuated creatinine, urea, CRP, and TNF-Î± level. Hyperglycemia and hypercholesterolemia linked kidney disorders were relieved.

Conclusion: In vivo oral administration with each extract declared suppression of cytokines mediated inflammation, vascular function leading to infiltration reduction of renal macrophage together with lowering in kidney indices and ameliorate renal tissues architectures in HG-HC rats.

Downloads

Download data is not yet available.

Author Biography

Hanan F Aly, Department of Therapeutic Chemistry, National Research Centre, Dokki, Giza, Egypt

Chemistry of Natural Compounds

References

Pickup JC. Inflammation and activated innate immunity in the pathogenesis of type 2 diabetes. Diabetes Care 2004;27(3):813-23.

El-Baz FK, Aly HF, Khalil WK, Booles HF, Saad SA. Jatropha curcas repairing effect on adhesion molecules, DNA damage and gene expression alteration in STZ-induced diabetic rats. Int J Pharm Bio Sci 2015;6:198-214.

Aly HF, Abd-Alla HI, Ali SA, Aba-Alez R, Abu-Krisha MT, Mamdouh MM. Statistical data analysis which result from the bio-diagnosis and bio-treatment of injured rats with the hyperlipidemia and hyperglycemia diseases. Asian J Pharm Clin Res 2016;9:122-30.

Abu-Gabal NS, Abd-Alla HI, Mohamed NZ, Aly HF, Shalaby NM. Phenolics composition, hypolipidemic, hypoglycemic and antioxidative effects of the leaves of Fortunella japonica (Thunb.) Swingle. Int J Pharm Pharm Sci 2015;7:55-63.

Mohamed NZ, Abd-Alla HI, Aly HF, Mantawy M, Ibrahim N, Hassan S. CCl4-induced hepatonephrotoxicity: protective effect of nutraceuticals on inflammatory factors and antioxidative status in rat. J Appl Pharm Sci 2014;4:87-100.

Krishna PM, Rao KN, Sandhya S, Banji D. A review on phytochemical, ethnomedical and pharmacological studies on genus Sophora, Fabaceae. Rev Bras Farm 2012;22:1145-54.

Meira M, da Silva EP, David JM, David JP. Review of the genus Ipomoea: traditional uses, chemistry and biological activities. Rev Bras Farmacogn2012; 22, http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000300031

Huang M, Deng S, Han Q, Zhao P, Zhou Q, Zheng S, et al. Hypoglycemic activity and the potential mechanism of the flavonoid rich extract from Sophora tonkinensis Gagnep in KK-Ay Mice. Front Pharmacol 2016;7: 288.

Zhang D, Liu S, Yao M, Liu X, Lai H. Effects of Vietnamese Sophora root on growth, adhesion, invasion and motility of melanoma cells. Afr J Tradit Complement Altern Med 2014;11:62â€“6.

Miyazaki K, Makino K, Iwadate E, Deguchi Y, Ishikawa F. Anthocyanins from purple sweet potato Ipomoea batatas cultivar Ayamurasaki suppress the development of atherosclerotic lesions and both enhancements of oxidative stress and soluble vascular cell adhesion molecule-1 in apolipoprotein E-deficient mice. J Agric Food Chem 2008;56:11485-92.

Efford N. Digital Image Processing-A Practical Introduction Using Java, First published, Pearson Education Limited, Addison Wesley, USA, 1st Edition, E-book, 2000.

Keedwell E, Narayanan A. Intelligent Bioinformatics: the application of artificial intelligence techniques to bioinformatics problems, John Wiley & Sons Ltd, The Atrium, Southern Gate, Chichester, West Sussex PO19 8SQ, England. E-book, 2005.

Shalaby NM, Abd-Alla HI, Aly HF, Albalawy MA, Shaker KH, Bouajila J. Preliminary in vitro and in vivo evaluation of antidiabetic activity of Ducrosia anethifolia Boiss. and its linear furanocoumarins. BioMed Res Int 2014;2014:1-13.

Abd-Alla HI, Albalawy MA, Aly HF, Shalaby NM, Shaker KH. Flavone composition and antihypercholesterolemic and antihyperglycemic activities of Chrysanthemum coronarium L. Z Naturforsch C 2014;69:199-208.

Bow ST. Pattern recognition and Image Preprocessing, 2nd ed., Marcel Dekker Ltd., New York, 2002.

Rasband WS. ImageJ: Image processing and analysis in java, National Institutes of Health, USA, 2012. http://rsbweb.nih.gov/ij/index.html.

Hwang SM, Kim JS, Lee YJ, Yoon JJ, Lee SM, Kang DG, et al. Anti-diabetic atherosclerosis effect of Prunella vulgaris in db/db mice with type 2 diabetes. Am J Chin Med 2012;40(5):937-51.

Awad HM, Abd-Alla HI, Mahmoud KH, El-Toumy SA. In vitro anti-nitrosative, antioxidant, and cytotoxicity activities of plant flavonoids: a comparative study. Med Chem Res 2014;23:3298-307.

Venkatesan B, Valente AJ, Das NA, Carpenter AJ, Yoshida T, Delafontaine JL, et al. CIKS (Act1 or TRAF3IP2) mediates high glucose-induced endothelial dysfunction. Cell Signal 2013;25: 359â€“71.

Warner EF, Zhang Q, Raheem KS, O'Hagan D, O'Connell MA, Kay CD. Common phenolic metabolites of flavonoids, but not their unmetabolized precursors, reduce the secretion of vascular cellular adhesion molecules by human endothelial cells. J Nutr 2016;146:465-73.

Kwon KB, Kim EK, Lim JG, Shin BC, Song YS, Seo E, et al. Sophorae radix extract inhibits high glucose-induced vascular cell adhesion molecule-1 up-regulation on endothelial cell line. Clin Chim Acta 2004;348:79-86.

Liu J, Zhang L, Ren Y, Gao Y, Kang L, Lu S. Matrine inhibits the expression of adhesion molecules in activated vascular smooth muscle cells. Mol Med Rep 2016;13: 2313-9.

Grace MH, Yousef GG, Gustafson SJ, Truong VD, Yencho GC, Lila MA. Phytochemical changes in phenolics, anthocyanins, ascorbic acid, and carotenoids associated with sweet potato storage and impacts on bioactive properties. Food Chem 2014;145:717-24.

Chao PY, Huang YP, Hsieh W. Inhibitive effect of purple sweet potato leaf extract and its components on cell adhesion and inflammatory response in human aortic endothelial cells. Cell Adh Migr 2013;7:237-45.

Sprague AH, Khalil RA. Inflammatory cytokines in vascular dysfunction and vascular disease. Biochem Pharmacol 2009;78:539â€“52.

Rada FH. Association of lipid fractions levels with cardiovascular disease. Asian J Pharm Clin Res2017; 10(3): 180-182.

Lima MC, Bitencourt M, Furtado AA, Oliveira H, Rocha A, Oliveira RM, da Silva-JÃºnior AA. Ipomoea asarifolia neutralizes inflammation induced by Tityus serrulatus scorpion venom. J Ethnopharmacol 2014;153:890-95.

Ferreira AA, Amaral FA, Duarte ID, Oliveira PM, Alves RB, Silveira D, et al. Antinociceptive effect from Ipomoea cairica extract. J Ethnopharmacol 2006;105:148-53.

Zhou H, Lutterodt H, Cheng Z, Yu LL. Anti-inflammatory and antiproliferative activities of trifolirhizin, a flavonoid from Sophora flavescens roots. J Agric Food Chem 2009;57: 4580â€“5.

Chi YS, Jong HG, Son KH, Chang HW, Kang SS, Kim HP. Effects of naturally occurring prenylated flavonoids on enzymes metabolizing arachidonic acid: cyclooxygenases and lipoxygenases. Biochem. Pharmacol 2001;62:1185â€“91.

Kim DW, Chi YS, Son KH, Chang HW, Kim JS, Kang S., Kim HP. Effects of sophoraflavanone G, a prenylated flavonoid from Sophora flavescens, on cyclooxygenase-2 and in vivo inflammatory response. Arch Pharm Res 2002; 25: 329-35.

Yoo H, Chae HS, Kim YM, Kang M, Ryu KH, Ahn, H.C., et al. Flavonoids and arylbenzofurans from the rhizomes and roots of Sophora tonkinensis with IL-6 production inhibitory activity. Bioorg Med Chem Lett 2014;24: 5644-7.

Wun ZY, Lin CF, Huang WC, Huang YL, Xu PY, Chang, W.T., et al. Antiinflammatory effect of sophoraflavanone G isolated from Sophora flavescens in lipopolysaccharide-stimulated mouse macrophages. Food Chem Toxicol 2013;62:255-61.

Choi BM, Oh GS, Lee JW, Mok JY, Kim DK, Jang SI. Prenylated chalcone from Sophora flavescens suppresses Th2 chemokine expression induced by cytokines via heme oxygenase-1 in human keratinocytes. Arch Pharm Res 2010;33:753-60.

Song CY, Zeng X, Wang Y, Shi J, Qian H, Zhang Y, et al. Sophocarpine attenuates toll-like receptor 4 in steatotic hepatocytes to suppress pro-inflammatory cytokines synthesis. J Gastroenterol Hepatol 2015;30:405-12.

Elsherbiny NM, Al-Gayyar MM. The role of IL-18 in type 1 diabetic nephropathy: The problem and future treatment. Cytokine 2016;81:15-22.

Wang SB, Jia JP. Oxymatrine attenuates diabetes-associated cognitive deficits in rats. Acta Pharmacol Sin 2014;35:331-8.

Shan Q, Zheng Y, Lu J, Zhang Z, Wu D, Fan S, et al. Purple sweet potato color ameliorates kidney damage via inhibiting oxidative stress mediated NLRP3 inflammasome activation in high fat diet mice. Food Chem Toxicol 2014;69:339-46.