PREPARATION AND EVALUATION OF CHEMICALLY INACTIVATED SALMONELLA ENTERITIDIS VACCINE IN CHICKENS

Mounir M El-safty; Hala Mahmoud; Eman Sa Zaki; Howaida I Abd-alla

doi:10.22159/ajpcr.2017.v10i11.20144

Authors

Mounir M El-safty Department of Evaluation of Inactivated Viral Poultry Vaccines, Central Laboratory for Evaluation of Veterinary Biologics, Abbassia 13181, Cairo, Egypt.
Hala Mahmoud Department of Evaluation of Inactivated Viral Poultry Vaccines, Central Laboratory for Evaluation of Veterinary Biologics, Abbassia 13181, Cairo, Egypt.
Eman Sa Zaki Department of Aerobic Bacterial Vaccines, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt.
Howaida I Abd-alla Department of Chemistry of Natural Compounds, Pharmaceutical Industries Division, National Research Centre, Dokki-Giza 12622,

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i11.20144

Keywords:

Salmonella enteritidis ghost, Specific pathogen-free, Vaccine, Enzyme-linked immunosorbent assay, Sodium dodecyl sulfate polyacrylamide gel electrophoresis

Abstract

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Â Objective: Salmonella enteritidis ghosts (SEGs) is a non-living empty bacterial cell envelopes which were generated using a different concentration of sodium hydroxide (NaOH) 6.4 mg/mL and evaluated as a vaccine candidate in specific pathogen-free (SPF) chicken. SEGs have been produced by chemical-mediated lysis and evaluated the potential efficacy of chemically induced SEG vaccine and its ability to induce protective immune responses against virulent S. enteritidis challenge in SPF chickens.

Methods: SPF chickens were divided into three groups: Group A (non-vaccinated control), Group B (vaccinated with prepared vaccine), and Group C (vaccinated with commercial vaccine).

Results: Vaccination of SPF chicken with SEGs induced higher immune responses before and after virulent challenge. SPF chicken vaccinated with SEGs showed increasing in serum enzyme-linked immunosorbent assay (ELISA) antibodies. During the vaccination period, Groups B and C showed higher serum antibody titer compared to Group A. The minimal inhibitory concentration (MIC) of NaOH was capable of inducing non-living SEGs, and it has successfully generated non-living SEGs by MIC of NaOH.

Conclusion: It is a one-step process which means easy manufacturing and low production cost compared to protein E-mediated lysis method. Chemically induced SEG vaccine is a highly effective method for inducing protective immunity. This study strongly suggests that SEGs will be a permissive vaccine, as the method of inhibition of S. enteritidis was safe and cheaper than other methods, and it gave a good protection.

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Author Biography

Mounir M El-safty, Department of Evaluation of Inactivated Viral Poultry Vaccines, Central Laboratory for Evaluation of Veterinary Biologics, Abbassia 13181, Cairo, Egypt.

Biological study

References

Krishna G, Kumar SS, Pranitha V, Alha M, Charaya S. Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: A study against Salmonella sp. Int J Pharm Pharm Sci 2015;7(10):84-8.

Clayton DJ, Bowen AJ, Hulme SD, Buckley AM, Deacon VL, Thomson NR et al. Analysis of the role of 13 major fimbrial subunits in colonisation of the chicken intestines by Salmonella enterica serovar Enteritidis reveals a role for a novel locus. BMC Microbiol 2008;8:1-15.

Thomas ME, Klinkenberg D, Ejeta G, Van Knapen F, Bergwerff AA, Stegeman JA, et al. Quantification of horizontal transmission of Salmonella enterica serovar Enteritidis bacteria in pair housed groups of laying hens. Appl Environ Microbiol 2009;75(19):6361-6.

Raffatellu M, Wilson RP, Chessa D, Andrews-Polymenis H, Tran QT, Lawhon S. Sip A, Sop A, Sop B, Sop D, and Sop E2 contribute to Salmonella enterica serotype typhimurium invasion of epithelial cells. Infect Immunol 2005;73(1):146-54.

Methner U, Barrow PA, Martin G, Meyer H. Comparative study of the protective effect against Salmonella colonisation in newly hatched SPF chickens using live, attenuated Salmonella vaccine strains, wild-type Salmonella strains or a competitive exclusion product. Int J Food Microbiol 1997;35(3):223-30.

Abu-Gabal NS, Howaida I, Abd-Alla HI, Mohamed NZ, Aly HF, Shalaby NM. Phenolics composition, hypolipidemic, hypoglycemic and antioxidative effects of the leaves of Fortunella japonica (Thunb.) Swingle. Int J Pharm Pharm Sci 2015;7:55-63.

El-Sawy ER, Abo-Salem HM, Zarie ES, Abd-Alla HI, El-Safty MM, Mandour AH. Synthesis and antiviral activity of novel ethyl 2-(3-heterocycle-1h-indol-1-yl) acetate derivatives. Int J Pharm Pharm Sci 2015;7(4):76-83.

Methner U, Barrow PA, Berndt A, Rychlik I. Salmonella enteritidis with double deletion in pho P fliC - A potential live Salmonella vaccine candidate with novel characteristics for use in chickens. Vaccine 2011;29(17):3248-53.

Zhang-Barber L, Turner AK, Barrow PA. Vaccination for control of Salmonella in poultry. Vaccine 1999;17(20-21):2538-45.

Fathy M, El-Safty MM, El-Jakee JK, Abd-Alla HI, Mahmoud H. Study the effect of Mycoplasma contamination of eggs used in virus titration and efficacy of some live attenuated poultry viral vaccines. Asian J Pharm Clin Res 2017;10(1):216-2.

Kwon HJ, Cho SH. Pathogenicity of SG 9R, a rough vaccine strain against fowl typhoid. Vaccine 2011;29(6):1311-8.

Jalava K, Hensel A, Szostak M, Resch S, Lubitz W. Bacterial ghosts as vaccine candidates for veterinary applications. J Control Release 2002;85(1):17-25.

Chaudhari AA, Jawale CV, Kim SW, Lee JH. Construction of a Salmonella gallinarum ghost as a novel inactivated vaccine candidate and its protective efficacy against fowl typhoid in chickens. Vet Res 2012;43:1-11.

Jawale CV, Chaudhari AA, Jeon BW, Nandre RM, Lee JH. Characterization of a novel inactivated Salmonella enterica serovar Enteritidis vaccine candidate generated using a modified cI857/Î» PR/gene E expression system. Infect Immunol 2012;80(4):1502-9.

Riedmann EM, Kyd JM, Smith AM, Gomez-Gallego S, Jalava K, Cripps AW, et al. Construction of recombinant S-layer proteins (rSbs A) and their expression in bacterial ghosts â€” A delivery system for the nontypeable Haemophilus influenzae antigen Omp26. FEMS Immunol Med Microbiol 2003;37:185-92.

Wang X, Lu C. Mice orally vaccinated with Edwardsiella tarda ghosts are significantly protected against infection. Vaccine 2009;27(10):1571-8.

Zhu W, Yang G, Zhang Y, Yuan J, An L. Generation of biotechnology-derived Flavobacterium columnare ghosts by phiX174 gene E-mediated inactivation and the potential as vaccine candidates against infection in grass carp. J Biomed Biotechnol 2012;2012:760730.

Li H, Li J, Cong XH, Duan YB, Li L, Wei PC, et al. A high-throughput, high-quality plant genomic DNA extraction protocol. Genet Mol Res 2013;12(4):4526-39.

Amara AA, Salem-Bekhit MM, Alanazi FK. Preparation of bacterial ghosts for E. coli JM109 using sponge-like reduced protocol. Asian J Biol Sci 2013;6:363-9.

Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press; 2001.

Tavares LC, Penna TC, Amaral AT. Synthesis and biological activity of nifuroxazide and analogs. Boll Chim Farm 1997;136(3):244-9.

Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227(5259):680-5.

Soumet C, Ermel G, Rose N, Rose V, Drouin P, Salvat G, et al. Evaluation of a multiplex PCR assay for simultaneous identification of Salmonella sp., Salmonella enteritidis and Salmonella typhimurium from environmental swabs of poultry houses. Lett Appl Microbiol 1999;28(2):113-7.

Park HJ, Oh S, Vinod N, Ji S, Noh HB, Koo JM, et al. Characterization of chemically-induced bacterial ghosts (BGs) using sodium hydroxide-

induced vibrio parahaemolyticus ghosts (VPGs). Int J Mol Sci 2016;17(11). pii: E1904.

Dias de Oliveira S, Rodenbusch C, Michael GB, Cardoso MR, Canal CW, Brandelli A. Detection of virulence genes in Salmonella enteritidis isolated from different sources. J Braz Microbiol 2003;34 Suppl 1:123-4.

Galan JE, Ginocchio C, Costeas P. Molecular and functional characterization of the Salmonella invasion gene inva: Homology of Inva to members of a new protein family. J Bacteriol 1992;174(13):4338-9.

Mehta V. Enteric fever in travelers: An updated insight. Asian J Pharm Clin Res 2016;9(S3):1-4.

Riedmann EM, Kyd JM, Cripps AW, Lubitz W. Bacterial ghosts as adjuvant particles. Expert Rev Vaccines 2007;6(2):241-53.

Kwon SR, Kang YJ, Lee DJ, Lee EH, Nam YK, Kim SK, et al. Generation of Vibrio anguillarum ghost by coexpression of phiX 174 lysis E gene and Staphylococcal nuclease a gene. Mol Biotechnol 2009;42(2):154-9.

Guo R, Geng S, Jiao H, Pan Z, Chen X, Jiao X. Evaluation of protective efficacy of a novel inactivated Salmonella pullorum ghost vaccine against virulent challenge in chickens. Vet Immunol Immunopathol 2016;173:27-33.

Vinod N, Oh S, Kim S, Choi CW, Kim SC, Jung CH. Chemically induced Salmonella enteritidis ghosts as a novel vaccine candidate against virulent challenge in a rat model. Vaccine 2014;32(42):3249-55.