FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS
DOI:
https://doi.org/10.22159/ijap.2018.v10s1.90Keywords:
1, 1-diphenyl-2-picrilhydrazyl, Antioxidant, Ferric reducing antioxidant power, Gacinia bancana MiqAbstract
Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.
Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric
reducing antioxidant power (FRAP), to identify the most active fraction.
Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. The
most active fraction was also shown to contain terpenoids.
Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methods
had antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that active
fraction contains terpenoids.
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References
Sen S, Chakraborty R, Sridhar C, Reddy YS, De B. Free radicals,
antioxidants, diseases and phytomedicines: Current status and future
prospect. Int J Pharm Sci Res 2010;3:91-100.
Simiati, IM. Test the Antioxidant Activity of Leaf Extract Garcinia
lateriflora Blume var. Javanica Boerl. With DDPH Method and
Identification of Identifikasi Chemical Compounds from Active
Fractions. Indonesia: Thesis, Faculty of Pharmacy, Universitas
Indonesia; 2012.
Nadheesha MK, Bamunuarachchi A, Edirisinghe EM, Weerasinghe WM.
Studies on antioxidant activity of Indian gooseberry fruit and seed. J Sci
Univ Kelaniya Sri Lanka 2007;3:83-92.
Wu N, Fu K, Fu YJ, Zu YG, Chang FR, Chen YH, et al. Antioxidant
activities of extracts and main components of pigeonpea (Cajanus
cajan (L.) Millsp.) leaves. Molecules 2009;14:1032-43.
Hartati S, Masrukhan, Cahyana H. Isolation of antioxidant compounds
in heaxane extract of Garcinia bancana Miq. J Kimia Ter Indones
;16:17-23.
Putri NL, Elya B, Puspitasari N. Antioxidant activity and lipoxygenase
inhibition test with total flavonoid content from Garcinia kydia
Roxburgh leaves extract. Pharmacogn J 2017;9:280-4.
Elya B, Katrin, Sauriasari R. Isolation, identification and evaluation of
compounds that are efficacious as antioxidants and antidiabetes from
familia Clusiaceae. Fac Pharm Univ Indones 2015.
Katrin H, Bendra A. The antioxidant activity of extract, fraction and
class of chemical compound leaves of Premna obngata Miq. (Aktivitas
antioksidan ekstrak, fraksi dan golongan senyawa kimia daun Premna
obngata Miq). Pharm Sci Res 2015;2:2-11.
Prabhune AM, Jadhav SN, Nandikar MD, Aparadh VT. Free radical
scavenging (DPPH) and ferric reducing ability (FRAP) of some
Commelinaceae members. Int J Biol Pharm All Sci 2013;2:1128-34.
Jan AT, Kamli MR, Murtaza I, Singh JB, Ali A, Haq QM. Dietary
flavonoid quercetin and associated health benefits-an overview. Food
Rev Int 2010;26:302-17.
Muharni M, Supriyatna S, Husein HB, Dahriyanus D. Phenolic compound
from the stem bark of Manggis Hutan (Garcinia bancana Miq.) and
their antioxidant activity. Indo J Chem 2009;9:321-7.
Antolovich M, Prenzler PD, Patsalides E, McDonald S, Robards K.
Methods for testing antioxidant activity. Analyst 2002;127:183-98.
Elya B, Rosmala D, Budiman MH. Antioxidant cream of Solanum
lycopersicum L. Int J Pharm Tech Res 2013;5:233-8.
Lu Y, Khoo TJ, Wiart C. Antioxidant activity determination of
citronellal and crude extracts of Cymbopogon citratus by 3 different
methods. Pharmacol Pharm 2014;5:395-400.
Fidrianny I, Aristya T, dan Hartati R. Antioxidant capacities of various
leaves extracts from three species of legumes and correlation with total
flavonoida, phenolic, carotenoid content. Int J Pharmacogn Phytochem
Res 2015;7:628-34.
Chanda S, Dave R. In vitro models for antioxidant activity evaluation
and some medicinal plants possessing antioxidant properties: An
overview. Afr J Microbiol Res 2009;3:981-96.
Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP)
as a measure of antioxidant power†the FRAP assay. Anal Biochem
;239:70-6.
Halvorsen BL, Holte K, Myhrstad MC, Barikmo I, Hvattum E,
Remberg SF, et al. A systematic screening of total antioxidants in
dietary plants. Am Soc Nutr Sci 2002;132:461-71.
Scapin G, Schmidt MM, Prestes R, Rosa CS. Phenoic compounds,
flavonoids and antioxidant activity of chia seed extract (Salvia
hispanica) obtained by different extraction conditions. Int Food Res J
;23:2341-6.
Elya B, Mun’im A, Hasiholan A, Marlin I. Antioxidant activities of
leaves extracts of three species of Garcinia. Int J Med Arom Plants
;2:691-3.
Nishaa S, Vishnupriya M, Sasikumar JM, Hephzibah PC,
Gopalakrishnan VK. Antioxidant activity of ethanolic extract of Maranta
arundinacea. L tuberous rhizomes. Asian J Pharm Clin Res 2012;5:85-8.
Sindhu S, Manorama S. Screening of Polycarpaea corymbosa Lam,
(Caryophylaceae) for its in vitro antioxidant activity. Asian J Pharm
Clin Res 2012;5:175-8.
Thambidurai Y, Sudarsanam D, Skm H, Ashok KT. Free radical
scavenging activity of marine sponges collected from Kovalam,
Chennai. Asian J Pharm Clin Res 2017;10:321-7.
Wagner H, Bladt S. Plant Drug Analysis. 2nd ed. Jerman: Springer;
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