DEVELOPMENT AND VALIDATION OF AN ANALYTICAL METHOD FOR THE DETERMINATION OF METHYLISOTHIAZOLINONE AND METHYLCHLOROISOTHIAZOLINONE IN COSMETIC PRODUCTS USING MATRIX SOLID-PHASE DISPERSION COUPLED TO GAS CHROMATOGRAPHY-MASS SPECTROMETRY

Authors

  • NURINA PRAPURANDINA Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia
  • HARMITA HARMITA Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia
  • HERMAN SURYADI Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia

DOI:

https://doi.org/10.22159/ijap.2021v13i2.40058

Keywords:

Methylisothiazolinone, Methylchloroisothiazolinone, Matrix solid-phase dispersion, Gas chromatography-mass spectrometry, Validation

Abstract

Objective: This study was to develop the first simultaneous method for quantification of MI and MCI by using matrix solid-phase dispersion (MSPD) as an extraction technique followed by gas chromatography-tandem mass spectrometry (GC-MS) in cosmetic products to support that law enforcement.

Methods: The MI and MCI were extracted from the cosmetic sample by using matrix solid-phase dispersion technique with alumina as solid sorbent and ethyl acetate as eluent. After being isolated, MI and MCI from the samples were analyzed using GC-MS equipped with DB-5MS capillary column.

Results: The validated method for both leave-on and rinse-off cosmetic showed that MI and MCI recoveries were between 97.87-103.15 %, relative standard deviation (RSD) values were lower than 11%, and limit of quantitation (LOQ) values for the leave-on product were 0.96 µg/ml and 1.95 µg/ml and for rinse-off products were 0.56 µg/ml and 1.49 µg/ml for MI and MCI, respectively.

Conclusion: This purposed analytical method for determining MI and MCI in cosmetic products using MSPD-GC-MS complies with the validation acceptance criteria.

Downloads

Download data is not yet available.

References

Alvarez Rivera G, Llompart M, Lores M, Garcia Jares C. Preservatives in cosmetics: regulatory aspects and analytical methods. In: Salvador A, Chisvert A. editors. Analysis of cosmetic products. 2nd ed. Oxford: Elsevier BV; 2018.

Magdaleno Tapial J, Valenzuela Onate C, Ortiz Salvador JM, Garcia Legaz Martinez M, Martinez Domenech A, Alonso Carpio M, et al. Contact allergy to isothiazolinones epidemic: current situation. Contact Dermatitis 2019;1–4.

Badan Pengawas Obat dan Makanan Republik Indonesia. Peraturan badan pengawas obat dan makanan republik Indonesia nomor 23 tahun 2019 tentang persyaratan teknis bahan kosmetika. Jakarta: Badan Pengawas Obat dan Makanan Republik Indonesia; 2019.

Ilankizhai RJ, Gayathri R, Vishnupriya V. Cosmetic contamination awareness among adolescent females. Asian J Pharm Clin Res 2016;9:117–20.

Balakrishnan J, Appalasamy JR. Skin infection and the global challenges: a review. Int J Pharm Pharm Sci 2016;8:1–3.

Balakrishnan J, Appalasamy JR, Verma RK. A study on awareness of skin infection among adults in Petaling district, Malaysia. Int J Pharm Pharm Sci 2016;8:136–9.

Silva V, Silva C, Soares P, Garrido EM, Borges F, Garrido J. Isothiazolinone biocides: chemistry, biological, and toxicity profiles. Molecules 2020;25:1–22.

Rafoth A, Gabriel S, Sacher F, Brauch HJ. Analysis of isothiazolinones in environmental waters by gas chromatography-mass spectrometry. J Chromatogr A 2007;1164:74–81.

Gopalan SV, Hasanah AN. MI-SPE, M-SPE and M-SPD recent application on solid-phase extraction for compound extraction of complex matrices. Int J Appl Pharm 2019;11:16–25.

Alvarez Rivera G, Dagnac T, Lores M, Garcia Jares C, Sanchez Prado L, Lamas JP, et al. Determination of isothiazolinone preservatives in cosmetics and household products by matrix solid-phase dispersion followed by high-performance liquid chromatography-tandem mass spectrometry. J Chromatogr A 2012;1270:41–50.

Park SK, Kwon JH. The fate of two isothiazolinone biocides, 5-chloro-2-methylisothiazol-3(2H)-one (CMI) and 2-methylisothiazol-3(2H)-one (MI), in liquid air fresheners and assessment of inhalation exposure. Chemosphere 2016;144:2270–6.

dos Santos EO, Gonzales JO, Ores JC, Marube LC, Caldas SS, Furlong EB, et al. Sand as a solid support in ultrasound-assisted MSPD: a simple, green and low-cost method for multi-residue pesticide determination in fruits and vegetables. Food Chem 2019;297:1–12.

International Conference on Harmonization (ICH). Validation of analytical procedures: text and methodology Q2 (R1). Geneva: International Conference on Harmonization; 2005.

Harmita. Analisis fisikokimia: Kromatografi. Vol. 2. Jakarta: Penerbit Buku Kedokteran EGC; 2015.

Dettmer Wilde K, Engewald W. Quantitative analysis. In: Dettmer Wilde K, Engewald W. editors. Practical gas chromatography. First. Berlin: Springer-Verlag; 2014. p. 271–302.

Caban M, Migowska N, Stepnowski P, Kwiatkowski M, Kumirska J. Matrix effects and recovery calculations in analyses of pharmaceuticals based on the determination of β-blockers and β-agonists in environmental samples. J Chromatogr A 2012;1258:117–27.

FAO-WHO. Codex alimentarius: Guidelines on performance criteria for methods of analysis for the determination of pesticide residues in food and feed. rome: food and agriculture organization of the united nations; 2017.

Wittenberg JB, Canas BJ, Zhou W, Wang PG, Rua D, Krynitsky AJ. Determination of methylisothiazolinone and methylchloroisothiazolinone in cosmetic products by ultra high-performance liquid chromatography with tandem mass spectrometry. J Sep Sci 2015;38:2983–8.

Tomas M, Agonia AS, Borges L, de Oliveira AP, de Oliveira RP. Isothiazolinones quantification in shampoo matrices: a matter of method optimization or stability driven by interactions? Cosmetics 2020;7:1-13.

Published

07-03-2021

How to Cite

PRAPURANDINA, N., HARMITA, H., & SURYADI, H. (2021). DEVELOPMENT AND VALIDATION OF AN ANALYTICAL METHOD FOR THE DETERMINATION OF METHYLISOTHIAZOLINONE AND METHYLCHLOROISOTHIAZOLINONE IN COSMETIC PRODUCTS USING MATRIX SOLID-PHASE DISPERSION COUPLED TO GAS CHROMATOGRAPHY-MASS SPECTROMETRY. International Journal of Applied Pharmaceutics, 13(2), 140–147. https://doi.org/10.22159/ijap.2021v13i2.40058

Issue

Section

Original Article(s)

Most read articles by the same author(s)

1 2 3 > >> 

Similar Articles

<< < 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.