DEVELOPMENT AND VALIDATION OF AN ANALYTICAL METHOD FOR THE DETERMINATION OF METHYLISOTHIAZOLINONE AND METHYLCHLOROISOTHIAZOLINONE IN COSMETIC PRODUCTS USING MATRIX SOLID-PHASE DISPERSION COUPLED TO GAS CHROMATOGRAPHY-MASS SPECTROMETRY

NURINA PRAPURANDINA; HARMITA HARMITA; HERMAN SURYADI

doi:10.22159/ijap.2021v13i2.40058

Authors

NURINA PRAPURANDINA Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia
HARMITA HARMITA Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia
HERMAN SURYADI Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia

DOI:

https://doi.org/10.22159/ijap.2021v13i2.40058

Keywords:

Methylisothiazolinone, Methylchloroisothiazolinone, Matrix solid-phase dispersion, Gas chromatography-mass spectrometry, Validation

Abstract

Objective: This study was to develop the first simultaneous method for quantification of MI and MCI by using matrix solid-phase dispersion (MSPD) as an extraction technique followed by gas chromatography-tandem mass spectrometry (GC-MS) in cosmetic products to support that law enforcement.

Methods: The MI and MCI were extracted from the cosmetic sample by using matrix solid-phase dispersion technique with alumina as solid sorbent and ethyl acetate as eluent. After being isolated, MI and MCI from the samples were analyzed using GC-MS equipped with DB-5MS capillary column.

Results: The validated method for both leave-on and rinse-off cosmetic showed that MI and MCI recoveries were between 97.87-103.15 %, relative standard deviation (RSD) values were lower than 11%, and limit of quantitation (LOQ) values for the leave-on product were 0.96 µg/ml and 1.95 µg/ml and for rinse-off products were 0.56 µg/ml and 1.49 µg/ml for MI and MCI, respectively.

Conclusion: This purposed analytical method for determining MI and MCI in cosmetic products using MSPD-GC-MS complies with the validation acceptance criteria.

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