DEVELOPMENT AND OPTIMIZATION OF THE WOUND HEALING ELECTROSPUN POLYURETHANE/COLLAGEN/PHYTOCERAMIDES NANOFIBERS USING THE BOX-BEHNKEN EXPERIMENTAL DESIGN) QUALITY BY DESIGN)

TASSNEIM M. EWEDAH; MOHAMED EL-NABARAWI; MAHMOUD H. TEAIMA; SAMMAR FATHY ELHABAL; KAMEL R. SHOUEIR; ABDALLAH M. HAMDY; AHMED ABDALLA

doi:10.22159/ijap.2024v16i5.51510

Authors

TASSNEIM M. EWEDAH Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Egyptian Russian University, Badr City, PO, Cairo-11829, Egypt. https://orcid.org/0000-0002-7565-301X
MOHAMED EL-NABARAWI Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, PO, Cairo-11562, Egyp.
MAHMOUD H. TEAIMA Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, PO, Cairo-11562, Egyp.
SAMMAR FATHY ELHABAL Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Modern University for Technology and Information (MTI), Mokattam, Cairo-11571, Egypt. https://orcid.org/0000-0002-7970-2288
KAMEL R. SHOUEIR ICPEES, Institut de Chimie et Procédé pour l’Energie, l’Environnement et la Santé, CNRS, UMR 7515, Université de Strasbourg, 25 Rue Becquerel-67087 Strasbourg Cedex 2, France.Institute of Nanoscience and Nanotechnology, Kafrelsheikh University, Kafrelsheikh-33516. https://orcid.org/0009-0005-0950-9334
ABDALLAH M. HAMDY Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Egyptian Russian University, Badr City, PO, Cairo-11829, Egypt https://orcid.org/0000-0002-8218-7512
AHMED ABDALLA Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Egyptian Russian University, Badr City, PO, Cairo-11829, Egypt. https://orcid.org/0000-0002-9437-2440

DOI:

https://doi.org/10.22159/ijap.2024v16i5.51510

Keywords:

Nanofiber, Wound healing, Box behnken design, Optimization, Quality by design

Abstract

Objective: This study aimed to develop and optimize polyurethane/collagen/phytoceramides nanofibers, a wound-healing drug delivery approach, using the electrospun technique. The objective was to enhance the effectiveness of nanofibers by optimizing the preparation process.

Methods: The box-behnken design was established to optimize the electrospinning instrument performance and, consequently, the nanofiber effectiveness. Response variables were diameter, zeta potential, and diffusion coefficient, while the experimental key factors were applied voltage, flow injection rate, and rotary collector speed of the electrospinning instrument. The optimized nanofibers were examined to ensure the validity of the optimization process.

Results: The study built prediction models for each response and employed a desirability function to suggest an optimum working level of each factor that guarantees minimum diameter, maximum zeta potential, and maximum diffusion coefficient. The desirability function suggested experimental conditions of 12.9 KV for the applied voltage, 1.3 ml/h for the injection flow rate, and a speed of 920 rpm for the rotary collector speed. The optimized formula proved satisfactory physicochemical properties regarding the nanofiber's infrared spectrum and wettability characteristics. The biomedical effectiveness of the optimized nanofibers showed increased anti-inflammatory potency up to 82.8±2.6% and a high wound closure rate of about 79%. Also, the stability study showed a nonsignificant change in response over the studied points.

Conclusion: The optimized nanofiber formula achieved the desired diameter, zeta potential, and diffusion coefficient. The results proved the Box-Behnken design approach's efficacy in enhancing the nanofiber formula's effectiveness and stability.

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