PROFILE OF ANTIBACTERIAL ACTIVITY OF FRACTIONS FROM METHANOL EXTRACTS OF GARCINIA LATISSIMA MIQ. FRUIT RIND
DOI:
https://doi.org/10.22159/ajpcr.2017.v10s5.23099Keywords:
Garcinia latissima Miq, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Zone of inhibition, Minimum inhibitory concentration, Bio-autographAbstract
Objectives: A previous study showed that methanol extracts of Garcinia latissima Miq. demonstrated antibacterial activity against Bacillus subtilis, Staphylococcus aureus, and Pseudomonas aeruginosa. The aim of this study was to obtain active antibacterial fractions from methanol extracts of G. latissima Miq.
Methods: Fractionation of extracts was performed on G60 silica gel column chromatography using n-hexane eluent, ethyl acetate, and methanol. Antibacterial tests were done using the paper disc method to determine the zone of inhibition, the microdilution method to determine the minimum inhibitory concentration (MIC), and a bioautographic test.
Results: Fractions A-E, and F had zones of inhibition against B. subtilis. Fractions A-E had zones of inhibition against S. aureus. Fractions C-E had zones of inhibition against P. aeruginosa. The bio-autograph test showed zones of inhibition on several bio-autographic spots, indicating that active compounds were obtained from the fractionation of methanol extract from G. latissima Miq. fruit rind. Fraction D's MIC against B. subtilis, S. aureus, and P. aeruginosa was 312.5 ppm. The MIC of Fraction C against B. subtilis and of Fraction R against P. aeruginosa also was 312.5 ppm.
Conclusion: Fraction D was the most active fraction against the three test bacteria.
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References
Srinivasan D, Nathan S, Suresh T, Lakshmana Perumalsamy P. Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine. J Ethnopharmacol 2001;74(3):217-20.
Jawetz E, Melnick JL, Adelberg EA, Carroll KC. Jawetz, Melnick & Adelberg’s Medical Microbiology. 27th ed. New York: Mc Graw Hill Education; 2016.
Ambarwati NS, Elya1 B, Malik A, Hanafi M. Phytochemical and antimicrobial studies on garcinia latissima miq. fruits extract. Asian J Pharm Clin Res 2017;7(10):230-2.
Ahuja S. Chromatography and Separation Science, Separation Science and Technology. Vol. 4. California: Elsevier Science; 2003.
Reid RG, Sarker SD. Isolationof natural products by low-pressure column chromatography. In: Sarker SD, Nahar L, editors. Natural Product Isolation Methods and Protocols. New York: Humana Press; 2012. p. 155-87. Available from: http://www.books.google.com/books?id=Ku2wPAAACAAJ.
Sarker SD, Latif Z, Gray AI. Natural Products Isolation. 2nd ed. New Jersey: Humana Press; 2006.
Kochuthressia KP, Britto SJ, Raphael RJ. In vitro antimicrobial evaluation of Kaempferia galanga L. Rhizome extract. Am J Biotechnol Mol Sci 2012;2(1):1-5. Available from: http://www.scihub.org/AJBMS/ PDF/2012/1/AJBMS-2-1-1-5.pdf.
Dewanjee S, Gangopadhyay M, Bhattacharya N, Khanra R, Dua TK. Bioautography and its scope in the field of natural product chemistry. J Pharm Anal 2015;5(2):75-84. Available from: http://www.dx.doi. org/10.1016/j.jpha.2014.06.002.
Messi BB, Ho R, Meli Lannang A, Cressend D, Perron K, Nkengfack AE, et al. Isolation and biological activity of compounds from Garcinia preussii. Pharm Biol 2014;52(6):706-11. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24824323.
Zubair M, Rızwan K, Rasool N, Afshan N, Shahid M, Ahmed VU, et al. Antimicrobial potential of various extract and fractions of leaves of Solanum nigrum. Int J Phytomed 2011;3(1):63-7.
Fernandes MR, Souza CR, Oliveira VA, Dias LT, Carvalho RR. Antioxidant and antimicrobial activities of Psidium guajava L. spray dried extracts. Ind Crops Prod 2014;60:39-44. Available from: http:// www.linkinghub.elsevier.com/retrieve/pii/S0926669014003288.
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