ALPHA-GLUCOSIDASE INHIBITING ACTIVITY FROM THE MOST ACTIVE FRACTION OF CALOPHYLLUM TOMENTOSUM, WIGHT LEAVES
DOI:
https://doi.org/10.22159/ajpcr.2017.v10s5.23101Keywords:
Diabetes mellitus, Calophyllum tomentosum Wight, Fractionation, Nil, Enzyme inhibitory assayAbstract
Objective: The purpose of this study was to obtain the inhibitory concentration, 50% value from the most active fraction of methanolic extract of leaf Calophyllum tomentosum, Wight, and to identify the group of compounds contained in the most active fraction.
Methods: The methanol extract was fractionated using the silica gel static phase, the n-hexane motion phase, ethyl acetate, and methanol enhanced by its polarity.
Results: There were nine selected fractions tested in inhibitory activity using an enzyme activity inhibitory assay.
Conclusions: The results showed the inhibition by the most active fraction is 51.288 μg/mL; this fraction contains of flavonoid, saponin, and terpenoid.
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